Purification by silica gel chromatography using dialysis tubing and characterization of sophorolipids produced from Candida bombicola grown on glucose and arachidonic acid

Biotechnology Letters

Volumn 29, Issue 2, 2007, Pages 267-272

  Shah, Sachin ^a   Prabhune, Asmita ^a  

^a NATIONAL CHEMICAL LABORATORY   (India)

Author keywords

19 HETE; 20 HETE; Arachidonic acid; Electrospray soft ionization mass spectroscopy; Silica gel chromatography; Sophorolipid

Indexed keywords

GEL PERMEATION CHROMATOGRAPHY; GLUCOSE; GROWTH KINETICS; MASS SPECTROMETRY; ORGANIC ACIDS; PURIFICATION; SILICA GEL;

ARACHIDONIC ACID; ELECTROSPRAY SOFT IONIZATION MASS SPECTROSCOPY; SILICA GEL CHROMATOGRAPHY; SOPHOROLIPIDS;

YEAST;

ARACHIDONIC ACID; GLUCAN; GLUCOSE; GLYCOLIPID; SILICON DIOXIDE; SOPHOROSE;

ARTICLE; BIOSYNTHESIS; CANDIDA; CHEMICAL STRUCTURE; CHEMISTRY; ELECTROSPRAY MASS SPECTROMETRY; GEL CHROMATOGRAPHY; INFRARED SPECTROSCOPY; INSTRUMENTATION; LIQUID CHROMATOGRAPHY; MASS FRAGMENTOGRAPHY; METABOLISM; METHODOLOGY; THIN LAYER CHROMATOGRAPHY;

ARACHIDONIC ACID; CANDIDA; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, LIQUID; CHROMATOGRAPHY, THIN LAYER; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; GLUCANS; GLUCOSE; GLYCOLIPIDS; MOLECULAR STRUCTURE; SILICON DIOXIDE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROSCOPY, FOURIER TRANSFORM INFRARED;

CANDIDA; STARMERELLA BOMBICOLA;

EID: 33846125009     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-006-9221-5     Document Type: Article

References (17)

1. Alonso-Galicia M, Falck JR, Reddy KM, Roman R (1999) 20-HETE agonist and antagonist in the renal circulation. Am J Physiol 277:790-796
2. Gorin APJ, Spenser JFT, Tulloch AP (1961) Hydroxy Fatty acid glycosides of sophorose from Torulopsis magnoliae. Can J Chem 39:846-855
3. Hommel RK, Ratledge C (1993) Biosynthetic mechanism of the low molecular weight surfactant and their precursor molecules. In: Kosaric N (ed) Biosurfactants: Production, Properties, Applications. Marcel Dekker Inc., New York, NY, pp 3-63
4. Ito S, Kinta M, Inoune S (1980) Growth of yeast on n-alkanes: inhibition by a lactonic sophorolipid produced by Torulopsis bombicola. Agric Biol Chem 44:2221-2223
5. Lasker JM, Chen WB, Wolf I, Bloswick BP, Wilson PD, Powell PK (2000) Formation of 20-hydroxyeicosatetraenoic acid, a vasoactive and natriuretic eicosanoids, in human kidney. J Biol Chem 275:4118-4126
6. MacLauf J, Rigaud M (1982) Open tubular glass capillary gas chromatography for separating eicosanoids. Meth Enzymol 86:612-631
7. Mantsch HH, Chapman D (1996) Infrared Spectroscopy of Biomolecules. Wiley, New York, NY. Yongmei Hu,
8. Lu-Kwang Ju (2001) Purification of lactonic sophorolipids by crystallization. J Biotechnol 87:263-272
9. Prabhune A, Fox SR, Ratledge C (2002) Transformation of arachidonic acid to 19-hydroxy and 20-hydroxyeicosatetraenoic acid using Candida bombicola. Biotechnol Lett 24:1041-1044
10. Phadtare S, Shah S, Wadgaonkar PP, Prabhune A, Sastry M (2004) Immobilization of Candida bombicola cells on free-standing organic-gold nanoparticle membranes and their use as enzyme sources in biotransformations. Biotechnol Prog 20:11817-1824
11. Scholz C, Mehta S, Bisht K, Guilmanov V, Kaplan D, Niocolosi R, Gross R (1998) Bioactivity of extracellular glycolipids- investigation of potential anti-cancer activity of sophorolipids and sophorolipid-derivatives. Polymer Prep 39:168-169
12. Silverstaein RM, Webster FX (1998) Spectrometric Identification of Organic Compounds, 6th edn. Wiley, New York
13. Stuwer O, Hommel R, Haferburg D, Kleber HP (1987) Production of crystalline surface-acytive glycolipids by a strain of Torulopsis apicola. J Biotechnol 6:259-269
14. Tulloch AP, Spencer JFT, Gorin PAJ (1962) The fermentation of long chain compounds by Torulopsis magnoliae. Can J Chem 10:1326-1338
15. Tulloch AP, Spencer JFT, Gorin PAJ (1968) A new hydroxy fatty acid sophoroside from Candida bogoriensis. Can J Chem 46:345-348
16. Rau U, Manzke C, Wagner F (1996) Influence of substrate supply on the production of sophorose lipid by Candida bombicola ATCC 22214. Biotechnol Lett 18:149-154
17. Yongmei Hu, Lu-Kwang Ju (2001) Purification of lactonic sophorolipids by crystallization. J Biotechnol 87:263-272