A new flavin radical signal in the Na+-pumping NADH:quinone oxidoreductase from Vibrio cholerae: An EPR/electron nuclear double resonance investigation of the role of the covalently bound flavins in subunits B and C

Journal of Biological Chemistry

Volumn 281, Issue 48, 2006, Pages 36482-36491

  Barquera, Blanca ^a   Ramirez Silva, Leticia ^b   Morgan, Joel E ^a   Nilges, Mark J ^c  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

^b UNIVERSIDAD NACIONAL AUTÓNOMA DE MÉXICO   (Mexico)

^c Illinois EPR Research Center   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; ENZYME KINETICS; MUTAGENS; PARAMAGNETIC RESONANCE; REDOX REACTIONS;

FLAVINS; NUCLEAR DOUBLE RESONANCE; OXIDOREDUCTASE;

ENZYMES;

ACRIFLAVINE; ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); AMINO ACID DERIVATIVE; FLAVINE MONONUCLEOTIDE; MUTANT PROTEIN; PROTEIN SUBUNIT; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE DEHYDROGENASE (UBIQUINONE); THREONINE;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; ELECTRON NUCLEAR DOUBLE RESONANCE; ELECTRON SPIN RESONANCE; ELUTION; GENE MUTATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; OXIDATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PURIFICATION; VIBRIO CHOLERAE;

BASE SEQUENCE; ELECTRON SPIN RESONANCE SPECTROSCOPY; FLAVINS; FLAVOPROTEINS; FREE RADICALS; LIGANDS; MODELS, STATISTICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; NA(+)-K(+)-EXCHANGING ATPASE; PROTEIN BINDING; QUINONE REDUCTASES; SODIUM; THREONINE; VIBRIO CHOLERAE;

VIBRIO CHOLERAE;

EID: 33845993613     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M605765200     Document Type: Article

References (25)

1. Barquera, B., Hellwig, P., Zhou, W., Morgan, J. E., Hase, C. C., Gosink, K. K., Nilges, M., Bruesehoff, P. J., Roth, A., Lancaster, C. R., and Gennis, R. B. (2002) Biochemistry 41, 3781-3789
2. Bogachev, A., Murtazina, R. A., and Skulachev, V. P. (1997) FEBS Lett. 409, 475-477
3. Bogachev, A., and Verkhovsky, M. (2005) Biochemistry (Mosc.) 70, 143-149
4. Hayashi, M., Nakayama, Y., and Unemoto, T. (2001) Biochim. Biophys. Acta 1505, 37-44
5. Tokuda, H., and Unemoto, T. (1984) J. Biol. Chem. 259, 7785-7790
6. Zhou, W., Bertsova, Y. V., Feng, B., Tsatsos, P., Verkhovskaya, M. L., Gennis, R. B., Bogachev, A. V., and Barquera, B. (1999) Biochemistry 38, 16246-16252
7. Hayashi, M., Nakayama, Y., and Unemoto, T. (1996) FEBS Lett. 381, 174-176
8. Pfenninger-Li, X., Albracht, S. P., van Belzen, R., and Dimroth, P. (1996) Biochemistry 35, 6233-6242
9. Barquera, B., Zhou, W., Morgan, J. E., and Gennis, R. B. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 10322-10324
10. Barquera, B., Nilges, M. J., Morgan, J. E., Ramirez-Silva, L., Zhou, W., and Gennis, R. B. (2004) Biochemistry 43, 12322-12330
11. Hayashi, M., Nakayama, Y., Yasui, M., Maeda, M., Furuishi, K., and Unemoto, T. (2001) FEBS Lett. 488, 5-8
12. Barquera, B., Hase, C. C., and Gennis, R. B. (2001) FEBS Lett. 492, 45-49
13. Turk, K., Puhar, A., Neese, F., Bill, E., Fritz, G., and Steuber, J. (2004) J. Biol. Chem. 279, 21349-21355
14. Nakayama, Y., Yasui, M., Sugahara, K., Hayashi, M., and Unemoto, T. (2000) FEBS Lett. 474, 165-168
15. Barquera, B., Morgan, J. E., Lukoyanov, D., Scholes, C. P., Gennis, R. B., and Nilges, M. J. (2003) J. Am. Chem. Soc. 125, 265-275
16. Bogachev, A., Bertsova, Y. V., Ruuge, E. K., Wikstrom, M., and Verkhovsky, M. I. (2002) Biochim. Biophys. Acta 1556, 113-120
17. Nilges, M. J. (1979) Electron Paramagnetic Resonance Studies of Low Symmetry Nickel(I) and Molybdenum(V) Complexes. Ph.D. thesis, University of Illinois at Urbana-Champaign
18. Wunsch, P., and Zumft, W. G. (2005) J. Bacteriol. 187, 1992-2001
19. Galli, C., MacArthur, R., Abu-Soud, H., Clark, P., Stuehr, D. J., and Brudvig, G. W. (1996) Biochemistry 38, 2804-2810
20. Kay, C. W., Feicht, R., Schulz, K., Sadewater, P., Sancar, A., Bacher, A., Mobius, K., Richter, G., and Weber, S. (1999) Biochemistry 38, 16740-16748
21. Edmonson, D. E. (1978) in Biological Magnetic Resonance (Berliner, L. J., and Reuben, J., eds) pp. 205-235, Plenum Publishing Corp., New York
22. Aliverti, A., Hagen, W. R., and Zanetti, G. (1995) FEBS Lett. 368, 220-224
23. Weber, S., Mobius, K., Richter, G., and Kay, C. W. (2001) J. Am. Chem. Soc. 123, 3790-3798
24. Ludwig, M. L., Pattridge, K. A., Metzger, A. L., Dixon, M. M., Eren, M., Feng, Y., and Swenson, R. P. (1997) Biochemistry 36, 1259-1280
25. Bogachev, A. V., Bertsova, Y. V., Bloch, D. A., and Verkhovsky, M. I. (2006) Biochemistry 45, 3421-3428