Beneficial effect of antioxidants in purified neurons derived from rat cortical culture

Brain Research

Volumn 1131, Issue 1, 2007, Pages 11-16

  Sagara, Jun ichi ^a   Fujiwara, Kyoko ^b   Sakakura, Yuki ^a,b   Sato, Hideyo ^c   Bannai, Shiro ^b   Makino, Nobuo ^a  

^a IBARAKI PREFECTURAL UNIVERSITY OF HEALTH SCIENCES   (Japan)

^b UNIVERSITY OF TSUKUBA   (Japan)

^c YAMAGATA UNIVERSITY   (Japan)

Author keywords

Antioxidant; Cell culture; Glutathione; Purified neuron; Serotonin

Indexed keywords

ALPHA TOCOPHEROL; ANTIOXIDANT; ASCORBIC ACID; BUTYLATED HYDROXYANISOLE; GLUTATHIONE; SEROTONIN; URIC ACID;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIOXIDANT ACTIVITY; ARTICLE; BRAIN CORTEX; CELL SURVIVAL; CONTROLLED STUDY; DNA PURIFICATION; EMBRYO; FETUS; FETUS (ANATOMY); GLUTATHIONE METABOLISM; NERVE CELL; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; RAT; SEROTONIN RELEASE;

ALPHA-TOCOPHEROL; ANIMALS; ANTIOXIDANTS; BUTYLATED HYDROXYANISOLE; CELL COMMUNICATION; CELL SURVIVAL; CELLS, CULTURED; CEREBRAL CORTEX; GLUTATHIONE; NEUROGLIA; NEURONS; OXIDATIVE STRESS; RATS; SEROTONIN;

RATTUS;

EID: 33845988785     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.brainres.2006.10.092     Document Type: Article

References (21)

1. Awasthi Y.C., Beutler E., and Srivastava S.K. Purification and properties of human erythrocyte glutathione peroxidase. J. Biol. Chem. 250 (1975) 5144-5149
2. Bannai S., and Tateishi N. Role of membrane transport in metabolism and function of glutathione in mammals. J. Membr. Biol. 89 (1986) 1-8
3. Bannai S., Tsukeda H., and Okumura H. Effect of antioxidants on cultured human diploid fibroblasts exposed to cystine-free medium. Biochem. Biophys. Res. Commun. 74 (1977) 1582-1588
4. Dalla Libera A., Scutari G., Boscolo R., Rigobello M.P., and Bindoli A. Antioxidant properties of clozapine and related neuroleptics. Free Radic. Res. 29 (1998) 151-157
5. Damier P., Hirsch E.C., Zhang P., Agid Y., and Javoy-Agid F. Glutathione peroxidase, glial cells and Parkinson's disease. Neuroscience 52 (1993) 1-6
6. Diaz-Hernandez J.I., Almeida A., Delgado-Esteban M., Fernandez E., and Bolanos J.P. Knockdown of glutamate-cysteine ligase by small hairpin RNA reveals that both catalytic and modulatory subunits are essential for the survival of primary neurons. J. Biol. Chem. 280 (2005) 38992-39001
7. Halliwell B. Role of free radicals in the neurodegenerative disease: therapeutic implications for antioxidant treatment. Drugs Aging 18 (2001) 685-716
8. Hertz L., Juurlink B.H.J., and Szuchet S. Cell cultures. In: Lajtha A. (Ed). Handbook of Neurochemistry. 2nd edition vol. 8 (1985), Plenum Press, New York 603-661
9. Kang J.Y., Kang H.J., Chung Y.K., Gwag B.J., and Noh J.S. 5-Hydroxytryptamine attenuates free radical injury in primary mouse cortical cultures. NeuroReport 12 (2001) 963-966
10. Khan J.Y., and Black S.M. Developmental changes in murine brain antioxidant enzymes. Pediatr. Res. 54 (2003) 77-82
11. Makino N., Mochizuki Y., Bannai S., and Sugita Y. Kinetic studies on the removal of extracellular hydrogen peroxide by cultured fibroblasts. J. Biol. Chem. 269 (1994) 1020-1025
12. Marshall K.A., Reiter R.J., Poeggeler B., Aruoma O.I., and Halliwell B. Evaluation of the antioxidant activity of melatonin in vitro. Free Radic. Biol. Med. 21 (1996) 307-315
13. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65 (1983) 55-63
14. Ratan R.R., Murphy T.H., and Baraban J.M. Oxidative stress induces apoptosis in embryonic cortical neurons. J. Neurochem. 62 (1994) 376-379
15. Reiter R.J., Tan D.X., Mayo J.C., Sainz R.M., Leon J., and Czarnocki Z. Melatonin as an antioxidant: biochemical mechanisms and pathophysiological implications in humans. Acta Biochim. Pol. 50 (2003) 1129-1146
16. Sagara J., Miura K., and Bannai S. Cystine uptake and glutathione level in fetal brain cells in primary culture and in suspension. J. Neurochem. 61 (1993) 1667-1671
17. Sagara J., Miura K., and Bannai S. Maintenance of neuronal glutathione by glial cells. J. Neurochem. 61 (1993) 1672-1676
18. Slivka A., Spina M.B., and Cohen G. Reduced and oxidized glutathione in human and monkey brain. Neurosci. Lett. 74 (1987) 112-118
19. Slivka A., Mytilineou C., and Cohen G. Histochemical evaluation of glutathione in brain. Brain Res. 409 (1987) 275-284
20. Takizawa S., Matsushima K., Shinohara Y., Ogawa S., Komatsu N., Utsunomiya H., and Watanabe K. Immunohistochemical localization of glutathione peroxidase in infarcted human brain. J. Neurol. Sci. 122 (1994) 66-73
21. Tietze F. Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues. Anal. Biochem. 27 (1969) 502-522