Mechanisms of small globular protein-induced plasma membrane permeability and cytotoxicity in U87-MG human malignant glioblastoma cells

Anticancer Research

Volumn 26, Issue 6 A, 2006, Pages 4033-4041

  Onishi, Hirokazu ^a   Jimi, Shiro ^a   Tsugu, Hitoshi ^a   Lee, Sannam ^a   Fukushima, Takeo ^a  

^a FUKUOKA UNIVERSITY   (Japan)

Author keywords

Ca2+ influx; Calcein; DAPI; Glioblastoma; LDH efflux; Permeability; Small globular protein; U87 MG

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; CALCEIN; CALCIUM ION; FLUORESCENT DYE; GLOBULAR PROTEIN; LACTATE DEHYDROGENASE;

ARTICLE; CALCIUM TRANSPORT; CANCER GROWTH; CELL DEATH; CELL MEMBRANE PERMEABILITY; CELL NUCLEUS; CELL ULTRASTRUCTURE; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; DRUG ACTIVITY; ELECTRON MICROSCOPY; ENZYME RELEASE; GLIOBLASTOMA; HUMAN; HUMAN CELL; PRIORITY JOURNAL;

ANTINEOPLASTIC AGENTS; BACTERIOCINS; CALCIUM; CELL LINE, TUMOR; CELL MEMBRANE; CELL MEMBRANE PERMEABILITY; GLIOBLASTOMA; HUMANS; INDOLES; L-LACTATE DEHYDROGENASE; PROTEIN FOLDING; PROTEINS;

EID: 33845956454     PISSN: 02507005     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (19)

1. Parker MW, Pattus F, Tucker AD and Tsernoglou D: Structure of the membrane-pore-forming fragment of colicin A. Nature 337: 93-96, 1989.
2. Lee S, Kiyota T, Kunitake T, Matsumoto E, Yamashita S and Anzai K: De novo design, synthesis, and characterization of a pore-forming small globular protein and its insertion into lipid bilayers. Biochemistry 36: 3782-3791, 1997.
3. Matsumoto E, Kiyota T, Lee S, Sugihara G, Yamashita S and Meno H: Study on the packing geometry, stoichiometry, and membrane interaction of three analogs related to a poreforming small globular protein. Biopolymers 56: 96-108, 2000.
4. Athanassiou H, Synodinou M, Maragoudakis E, Paraskevaidis M, Verigos C, Misailidou D, Antonadou D, Saris G, Beroukas K and Karageorgis P: Randomized Phase II Study of Temozolomide and Radiotherapy Compared With Radiotherapy Alone in Newly Diagnosed Glioblastoma Multiforme. J Clin Oncol 23: 2372-2377, 2005.
5. Liu Y, Peterson DA, Kimura H and Schubert D: Mechanism of cellular 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction. J Neurochem 69: 581-593, 1997.
6. Cory AH, Owen TC, Barltrop JA and Cory JG: Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. Cancer Commun 3: 207-212, 1991.
7. Schnedl W, Mikelsaar AV, Breitenbach M and Dann O: DIPI and DAPI: fluorescence banding with only negligible fading. Hum Genet 36: 167-172, 1977.
8. Poulin N, Harrison A and Palcic B: Quantitative precision of an automated image cytometric system for the measurement of DNA content and distribution in cells labeled with fluorescent nucleic acid stains. Cytometry 16: 227-235, 1994.
9. Wang XM, Terasaki PI, Rankin GW Jr, Chia D, Zhong HP and Hardy S: A new microcellular cytotoxicity test based on calcein AM release. Hum Immunol 37: 264-270, 1993.
10. Levesque A, Paquet A and Page M: Measurement of tumor necrosis factor activity by flow cytometry. Cytometry 20: 181-184, 1995.
11. Korzeniewski C and Callewaert DM: An enzyme-release assay for natural cytotoxicity. J Immunol Methods 64: 313-320, 1983.
12. Decker T and Lohmann-Matthes ML: A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity. J Immunol Methods 115: 61-69, 1988.
13. Orrenius S, McCabe MJ Jr and Nicotera P: Ca(2+)-dependent mechanisms of cytotoxicity and programmed cell death. Toxicol Lett 64-65 Spec No: 357-364, 1992.
14. 2+ indicators with greatly improved fluorescence properties. J Biol Chem 260: 3440-3450, 1985.
15. Tsien RY, Pozzan T and Rink TJ: Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator. J Cell Biol 94: 325-334, 1982.
16. Ohmori N, Niidome T, Kiyota T, Lee S, Sugihara G and Wada A: Importance of hydrophobic region in amphiphilic structures of alpha-helical peptides for their gene transfer-ability into cells. Biochem Biophys Res Commun 245: 259-265, 1998.
17. Ellerby HM, Lee S, Ellerby LM, Chen S, Kiyota T, del Rio G, Sugihara G, Sun Y, Bredesen DE, Arap W and Pasqualini R: An artificially designed pore-forming protein with anti-tumor effects. J Biol Chem 278: 35311-35316, 2003.
18. Jimi S, Uchiyama M, Takaki A, Suzumiya J and Hara S: Mechanisms of cell death induced by cadmium and arsenic. Ann NY Acad Sci 1011: 325-331, 2004.
19. Tsugu H, Onishi H, Fukushima T and Lee S: Anti-tumor activity exhibited by a de novo designed Small Globular Protein (SGP): An in vivo experimental design. Anticancer Res 26: 4043-4046, 2006.