Phosphorylated vasodilator-stimulated phosphoprotein is localized on mitotic spindles of the gastric cancer cell line SGC-7901

World Journal of Gastroenterology

Volumn 12, Issue 46, 2006, Pages 7478-7481

  Tao, Yan ^a   Chen, Yong Chang ^a   Wang, Ying ^a   Zhang, Zhi Jian ^a   Xu, Wen Rong ^a  

^a JIANGSU UNIVERSITY   (China)

Author keywords

Gastric cancer cell; Localization; Mitotic spindle; Phosphorylated vasodilator stimulated phosphoprotein

Indexed keywords

ALPHA TUBULIN; CYCLIC AMP DEPENDENT PROTEIN KINASE; CYCLIC GMP DEPENDENT PROTEIN KINASE; CYTOSKELETON PROTEIN; N [2 (4 BROMOCINNAMYLAMINO)ETHYL] 5 ISOQUINOLINESULFONAMIDE; SERINE; VASODILATOR STIMULATED PHOSPHOPROTEIN;

ANAPHASE; ARTICLE; CANCER CELL CULTURE; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE MICROSCOPY; INTERPHASE; METAPHASE; MITOSIS; MITOSIS SPINDLE; PROPHASE; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; STOMACH CANCER;

EID: 33845908722     PISSN: 10079327     EISSN: None     Source Type: Journal    
DOI: 10.3748/wjg.v12.i46.7478     Document Type: Article

References (26)

1. Heald R, Tournebize R, Habermann A, Karsenti E, Hyman A. Spindle assembly in Xenopus egg extracts: respective roles of centrosomes and microtubule self-organization. J Cell Biol 1997; 138: 615-628
2. Schnackenberg BJ, Khodjakov A, Rieder CL, Palazzo RE. The disassembly and reassembly of functional centrosomes in vitro. Proc Natl Acad Sci USA 1998; 95: 9295-9300
3. Zheng Y, Wong ML, Alberts B, Mitchison T. Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex. Nature 1995; 378: 578-583
4. Aizawa H, Emori Y, Murofushi H, Kawasaki H, Sakai H, Suzuki K. Molecular cloning of a ubiquitously distributed microtubule-associated protein with Mr 190,000. J Biol Chem 1990; 265: 13849-13855
5. 3 Mr microtubule-associated protein (MAP4) contains a domain homologous to the microtubule-binding domains of neuronal MAP2 and tau. J Cell Sci 1991; 98 (Pt 1): 27-36
6. Tokuraku K, Katsuki M, Nakagawa H, Kotani S. A new model for microtubule-associated protein (MAP)-induced microtubule assembly. The Pro-rich region of MAP4 promotes nucleation of microtubule assembly in vitro. Eur J Biochem 1999; 259: 158-166
7. Markert T, Krenn V, Leebmann J, Walter U. High expression of the focal adhesion- and microfilament-associated protein VASP in vascular smooth muscle and endothelial cells of the intact human vessel wall. Basic Res Cardiol 1996; 91: 337-343
8. Draijer R, Vaandrager AB, Nolte C, de Jonge HR, Walter U, van Hinsbergh VW. Expression of cGMP-dependent protein kinase I and phosphorylation of its substrate, vasodilator-stimulated phosphoprotein, in human endothelial cells of different origin. Circ Res 1995; 77: 897-905
9. Haffner C, Jarchau T, Reinhard M, Hoppe J, Lohmann SM, Walter U. Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP. EMBO J 1995; 14: 19-27
10. Reinhard M, Halbrugge M, Scheer U, Wiegand C, Jockusch BM, Walter U. The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and focal contacts. EMBO J 1992; 11: 2063-2070
11. Rottner K, Behrendt B, Small JV, Wehland J. VASP dynamics during lamellipodia protrusion. Nat Cell Biol 1999; 1: 321-322
12. Svitkina TM, Bulanova EA, Chaga OY, Vignjevic DM, Kojima S, Vasiliev JM, Borisy GG. Mechanism of filopodia initiation by reorganization of a dendritic network. J Cell Biol 2003; 160: 409-421
13. Mack GJ, Compton DA. Analysis of mitotic microtubule-associated proteins using mass spectrometry identifies astrin, a spindle-associated protein. Proc Natl Acad Sci USA 2001; 98: 14434-14439
14. Katsuki M, Tokuraku K, Murofushi H, Kotani S. Functional analysis of microtubule-binding domain of bovine MAP4. Cell Struct Funct 1999; 24: 337-344
15. Ferralli J, Doll T, Matus A. Sequence analysis of MAP2 function in living cells. J Cell Sci 1994; 107 (Pt 11): 3115-3125
16. Gustke N, Trinczek B, Biernat J, Mandelkow EM, Mandelkow E. Domains of tau protein and interactions with microtubules. Biochemistry 1994; 33: 9511-9522
17. Aizawa H, Emori Y, Mori A, Murofushi H, Sakai H, Suzuki K. Functional analyses of the domain structure of microtubule-associated protein-4 (MAP-U). J Biol Chem 1991; 266: 9841-9846
18. Mori A, Aizawa H, Saido TC, Kawasaki H, Mizuno K, Murofushi H, Suzuki K, Sakai H. Site-specific phosphorylation by protein kinase C inhibits assembly-promoting activity of microtubule-associated protein 4. Biochemistry 1991; 30: 9341-9346
19. Laurent V, Loisel TP, Harbeck B, Wehman A, Grebe L, Jockusch BM, Wehland J, Gertler FB, Carlier MF. Role of proteins of the Ena/VASP family in actin-based motility of Listeria monocytogenes. J Cell Biol 1999; 144: 1245-1258
20. Glover DM, Leibowitz MH, McLean DA, Parry H. Mutations in aurora prevent centrosome separation leading to the formation of monopolar spindles. Cell 1995; 81: 95-105
21. Blangy A, Lane HA, d'Herin P, Harper M, Kress M, Nigg EA. Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell 1995; 83: 1159-1169
22. Wentworth JK, Pula G, Poole AW. Vasodilator-stimulated phosphoprotein (VASP) is phosphorylated on Ser157 by protein kinase C-dependent and -independent mechanisms in thrombin-stimulated human platelets. Biochem J 2006; 393: 555-564
23. Lawrence DW, Pryzwansky KB. The vasodilator-stimulated phosphoprotein is regulated by cyclic GMP-dependent protein kinase during neutrophil spreading. J Immunol 2001; 166: 5550-5556
24. Smolenski A, Poller W, Walter U, Lohmann SM. Regulation of human endothelial cell focal adhesion sites and migration by cGMP-dependent protein kinase I. J Biol Chem 2000; 275: 25723-25732
25. Wei L, Muller S, Ouyang J, Stoltz JF, Wang X. Changes of vasodilator-stimulated phosphoprotein (VASP) and its phosphorylation in endothelial cells exposed to laminar flow. Clin Hemorheol Microcirc 2003; 28: 113-120
26. Chen L, Daum G, Chitaley K, Coats SA, Bowen-Pope DF, Eigenthaler M, Thumati NR, Walter U, Clowes AW. Vasodilator-stimulated phosphoprotein regulates proliferation and growth inhibition by nitric oxide in vascular smooth muscle cells. Arterioscler Thromb Vasc Biol 2004; 24: 1403-1408