메뉴 건너뛰기




Volumn 5, Issue 1, 2007, Pages 69-83

SAGE of the developing wheat caryopsis

Author keywords

Abundant transcripts; Expression profiling; Hexaploid wheat; SAGE; Seed development

Indexed keywords

PROTEIN KINASE; VEGETABLE PROTEIN;

EID: 33845729926     PISSN: 14677644     EISSN: 14677652     Source Type: Journal    
DOI: 10.1111/j.1467-7652.2006.00218.x     Document Type: Article
Times cited : (46)

References (63)
  • 1
    • 0028388161 scopus 로고
    • Transcripts encoding an oleosin and a dormancy-related protein are present in both the aleurone layer and the embryo of developing barley (Hordeum vulgare L.) seeds
    • Alan, R., Opsahl-Ferstad, H., Linnestad, C. and Olsen, O. (1994) Transcripts encoding an oleosin and a dormancy-related protein are present in both the aleurone layer and the embryo of developing barley (Hordeum vulgare L.) seeds. Plant J. 5, 385-396.
    • (1994) Plant J. , vol.5 , pp. 385-396
    • Alan, R.1    Opsahl-Ferstad, H.2    Linnestad, C.3    Olsen, O.4
  • 2
    • 0028041636 scopus 로고
    • A novel embryo-specific barley cDNA clone encodes a protein with homologies to bacterial glucose and ribitol dehydrogenase
    • Alexander, R., Alamillio, J., Salamini, F. and Bartels, D. (1994) A novel embryo-specific barley cDNA clone encodes a protein with homologies to bacterial glucose and ribitol dehydrogenase. Planta. 192, 519-525.
    • (1994) Planta , vol.192 , pp. 519-525
    • Alexander, R.1    Alamillio, J.2    Salamini, F.3    Bartels, D.4
  • 3
    • 51249171246 scopus 로고
    • Nuclear DNA content of some important plant species
    • Arumuganathan, K. and Earle, E. (1991) Nuclear DNA content of some important plant species. Plant Mol. Biol. Rep. 9, 208218.
    • (1991) Plant Mol. Biol. Rep. , vol.9 , pp. 208218
    • Arumuganathan, K.1    Earle, E.2
  • 4
    • 0036634210 scopus 로고    scopus 로고
    • From elicitins to lipid-transfer proteins: A new insight in cell signalling involved in plant defence mechanisms
    • Blein, J., Coutos-Thevenot, P., Marion, D. and Ponchet, M. (2002) From elicitins to lipid-transfer proteins: a new insight in cell signalling involved in plant defence mechanisms. Trends Plant Sci. 7, 293-296.
    • (2002) Trends Plant Sci. , vol.7 , pp. 293-296
    • Blein, J.1    Coutos-Thevenot, P.2    Marion, D.3    Ponchet, M.4
  • 5
    • 23644437867 scopus 로고    scopus 로고
    • Wheat non-specific lipid transfer protein genes display a complex pattern of expression in developing seeds
    • Boutrot, F., Guirao, A., Alary, R., Joudrier, P. and Gautier, M.-F. (2005) Wheat non-specific lipid transfer protein genes display a complex pattern of expression in developing seeds. Biochim. Biophys. Acta. 1730, 114-125.
    • (2005) Biochim. Biophys. Acta , vol.1730 , pp. 114-125
    • Boutrot, F.1    Guirao, A.2    Alary, R.3    Joudrier, P.4    Gautier, M.-F.5
  • 6
    • 0036545474 scopus 로고    scopus 로고
    • A multigene family of glycosyltransferases in a model plant, Arabidopsis thaliana
    • Bowles, D. (2001) A multigene family of glycosyltransferases in a model plant, Arabidopsis thaliana. Biochem. Soc. Trans. 30, 301-306.
    • (2001) Biochem. Soc. Trans. , vol.30 , pp. 301-306
    • Bowles, D.1
  • 8
    • 0028541137 scopus 로고
    • cDNA cloning and nucleotide sequence of alpha-1 and alpha-2 thionins from hexaploid wheat endosperm
    • Castagnaro, A.C.M., Carbonero, P. and Garcia-Olmedo, F. (1994) cDNA cloning and nucleotide sequence of alpha-1 and alpha-2 thionins from hexaploid wheat endosperm. Plant Physiol. 106, 1221-1222.
    • (1994) Plant Physiol. , vol.106 , pp. 1221-1222
    • Castagnaro, A.C.M.1    Carbonero, P.2    Garcia-Olmedo, F.3
  • 10
    • 27744463090 scopus 로고    scopus 로고
    • Systematic spatial analysis of gene expression during wheat caryopsis development
    • Drea, S., Leader, D., Arnold, B., Shaw, P., Dolan, L. and Doonan, J. (2005) Systematic spatial analysis of gene expression during wheat caryopsis development. Plant Cell, 17, 2172-2185.
    • (2005) Plant Cell , vol.17 , pp. 2172-2185
    • Drea, S.1    Leader, D.2    Arnold, B.3    Shaw, P.4    Dolan, L.5    Doonan, J.6
  • 11
    • 0036043201 scopus 로고    scopus 로고
    • Comparative genomics in the grass family: Molecular characterization of grass genome structure and evolution
    • Feuillet, C. and Keller, B. (2002) Comparative genomics in the grass family: molecular characterization of grass genome structure and evolution. Ann. Bot. 89, 3-10.
    • (2002) Ann. Bot. , vol.89 , pp. 3-10
    • Feuillet, C.1    Keller, B.2
  • 13
    • 33845799744 scopus 로고    scopus 로고
    • Patterns of cell division revealed by transcriptional regulation of genes during the cell cycle in plants
    • Forbert, P., Coen, E., Murphy, G. and Doonan, J. (1999) Patterns of cell division revealed by transcriptional regulation of genes during the cell cycle in plants. EMBO J. 1, 271-285.
    • (1999) EMBO J. , vol.1 , pp. 271-285
    • Forbert, P.1    Coen, E.2    Murphy, G.3    Doonan, J.4
  • 15
    • 0037023349 scopus 로고    scopus 로고
    • A draft sequence of the rice genome (Oryza sativa L. ssp. japonica)
    • Goff, S., Ricke, D., Lan, T. and et al. (2002) A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science, 296, 92-100.
    • (2002) Science , vol.296 , pp. 92-100
    • Goff, S.1    Ricke, D.2    Lan, T.3
  • 16
    • 1642506317 scopus 로고    scopus 로고
    • Robust-LongSage (RL-SAGE): A substantially improved LongSAGE method for gene discovery and transcriptome analysis
    • Gowda, M., Jantasuriyarat, C., Dean, R. and Wang, G. (2004) Robust-LongSage (RL-SAGE): a substantially improved LongSAGE method for gene discovery and transcriptome analysis. Plant Physiol. 134, 890-897.
    • (2004) Plant Physiol. , vol.134 , pp. 890-897
    • Gowda, M.1    Jantasuriyarat, C.2    Dean, R.3    Wang, G.4
  • 17
    • 33845774854 scopus 로고    scopus 로고
    • A wheat mRNA encodes a putative amino acid selective channel protein and is upregulated by ABA, cold and dehydration
    • Holppa, L. and Walker-Simmons, M. (2000) A wheat mRNA encodes a putative amino acid selective channel protein and is upregulated by ABA, cold and dehydration. American Society of Plant Biologists.
    • (2000) American Society of Plant Biologists
    • Holppa, L.1    Walker-Simmons, M.2
  • 18
    • 33845728577 scopus 로고    scopus 로고
    • Isolation of beta-purothionin cdna (accession, af004018) from hexaploid wheat
    • Hughes, P., Llewellyn, D. and Whitecross, M. (1997) Isolation of beta-purothionin cdna (accession, af004018) from hexaploid wheat. Plant Physiol. 114, 1568.
    • (1997) Plant Physiol. , vol.114 , pp. 1568
    • Hughes, P.1    Llewellyn, D.2    Whitecross, M.3
  • 19
    • 0029240342 scopus 로고
    • Molecular characterization of proteins in protein-body membranes that disappear most rapidly during transformation of protein bodies into vacuoles
    • Inonue, K., Motozak, I.A., Takeuchi, Y., Nishimura, M. and Hara-Nishimura, I. (1995) Molecular characterization of proteins in protein-body membranes that disappear most rapidly during transformation of protein bodies into vacuoles. Plant J. 7, 235-243.
    • (1995) Plant J. , vol.7 , pp. 235-243
    • Inonue, K.1    Motozak, I.A.2    Takeuchi, Y.3    Nishimura, M.4    Hara-Nishimura, I.5
  • 20
    • 0035018616 scopus 로고    scopus 로고
    • A plant plasma membrane ATP binding cassette-type transporter is involved in antifungal terpenoid secretion
    • Jasinski, M., Stukkens, Y., Degand, H., Purnelle, B., Marchand-Brynaert, J. and Boutry, M. (2001) A plant plasma membrane ATP binding cassette-type transporter is involved in antifungal terpenoid secretion. Plant Cell, 13, 1095-1107.
    • (2001) Plant Cell , vol.13 , pp. 1095-1107
    • Jasinski, M.1    Stukkens, Y.2    Degand, H.3    Purnelle, B.4    Marchand-Brynaert, J.5    Boutry, M.6
  • 21
    • 0032573156 scopus 로고    scopus 로고
    • δ-Tonoplast intrinsic protein defines unique plant vacuole functions
    • Jauh, G., Fischer, A., Grimes, H., Ryan, C. and Rogers, J. (1998) δ-Tonoplast intrinsic protein defines unique plant vacuole functions. Proc. Natl Acad. Sci. USA, 95, 12 995-12 999.
    • (1998) Proc. Natl Acad. Sci. USA , vol.95 , pp. 12995-12999
    • Jauh, G.1    Fischer, A.2    Grimes, H.3    Ryan, C.4    Rogers, J.5
  • 23
    • 0028371792 scopus 로고
    • Extensin: Repetitive motifs, functional sites, post-translational codes, and phylogeny
    • Kieliszewski, M.J. and Lamport, D.T.A. (1994) Extensin: repetitive motifs, functional sites, post-translational codes, and phylogeny. Plant J. 5, 157-172.
    • (1994) Plant J. , vol.5 , pp. 157-172
    • Kieliszewski, M.J.1    Lamport, D.T.A.2
  • 24
    • 0024645050 scopus 로고
    • Characterization of embryo globulins encoded by the maize Glb genes
    • Kriz, A. (1989) Characterization of embryo globulins encoded by the maize Glb genes. Biochem. Genet. 27, 239-251.
    • (1989) Biochem. Genet. , vol.27 , pp. 239-251
    • Kriz, A.1
  • 27
    • 84971031522 scopus 로고
    • Early stages in the development of wheat endosperm. The changes from free nuclear to cellular endosperm
    • Mares, D., Norstog, K. and Stone, B. (1975) Early stages in the development of wheat endosperm. The changes from free nuclear to cellular endosperm. Aust. J. Bot. 23, 311-326.
    • (1975) Aust. J. Bot. , vol.23 , pp. 311-326
    • Mares, D.1    Norstog, K.2    Stone, B.3
  • 28
    • 0029825193 scopus 로고    scopus 로고
    • A barley (Hordeum vulgare L.) LEA3 protein, HVA1, is abundant in protein storage vacuoles
    • Marttila, S., Tenhola, T. and Mikkonen, A. (1996) A barley (Hordeum vulgare L.) LEA3 protein, HVA1, is abundant in protein storage vacuoles. Planta, 199, 602-611.
    • (1996) Planta , vol.199 , pp. 602-611
    • Marttila, S.1    Tenhola, T.2    Mikkonen, A.3
  • 29
    • 0037165995 scopus 로고    scopus 로고
    • An LRR receptor kinase involved in perception of a peptide plant hormone, phytosulfokine
    • Matsubayashi, Y., Ogawa, M., Morita, A. and Sakagami, Y. (2002) An LRR receptor kinase involved in perception of a peptide plant hormone, phytosulfokine. Science, 296, 1470-1472.
    • (2002) Science , vol.296 , pp. 1470-1472
    • Matsubayashi, Y.1    Ogawa, M.2    Morita, A.3    Sakagami, Y.4
  • 30
    • 0033375845 scopus 로고    scopus 로고
    • Transcript profiling in rice (Oryza sativa L.) seedlings using serial analysis of gene expression (SAGE)
    • Matsumura, H., Nirasawa, S. and Ryohei, T. (1999) Transcript profiling in rice (Oryza sativa L.) seedlings using serial analysis of gene expression (SAGE). Plant J. 20, 719-726.
    • (1999) Plant J. , vol.20 , pp. 719-726
    • Matsumura, H.1    Nirasawa, S.2    Ryohei, T.3
  • 31
    • 0026002971 scopus 로고
    • The viviparous-1 developmental gene of maize encodes a novel transcriptional activator
    • McCarty, D., Hattori, T., Carson, C., Vasil, V., Lazar, M. and Vasil, J. (1991) The viviparous-1 developmental gene of maize encodes a novel transcriptional activator. Cell, 66, 895-905.
    • (1991) Cell , vol.66 , pp. 895-905
    • McCarty, D.1    Hattori, T.2    Carson, C.3    Vasil, V.4    Lazar, M.5    Vasil, J.6
  • 33
    • 0027507001 scopus 로고
    • Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens
    • Molina, A., Segura, A. and Garcia-Olmedo, F. (1993) Lipid transfer proteins (nsLTPs) from barley and maize leaves are potent inhibitors of bacterial and fungal plant pathogens. FEBS Lett. 316, 119-122.
    • (1993) FEBS Lett. , vol.316 , pp. 119-122
    • Molina, A.1    Segura, A.2    Garcia-Olmedo, F.3
  • 34
    • 0034472919 scopus 로고    scopus 로고
    • New insights into the mechanisms of lipid-body biogenesis in plants and other organisms
    • Murphy, D., Hernendez-Pinzon, I., Patel, K., Hope, R. and McLauchlan, J. (2000) New insights into the mechanisms of lipid-body biogenesis in plants and other organisms. Biochem. Soc. Trans. 28, 710-711.
    • (2000) Biochem. Soc. Trans. , vol.28 , pp. 710-711
    • Murphy, D.1    Hernendez-Pinzon, I.2    Patel, K.3    Hope, R.4    McLauchlan, J.5
  • 35
    • 14844337487 scopus 로고    scopus 로고
    • Genome-wide profiling of stored mRNA in Arabidopsis thaliana seed germination: Epigenetic and genetic regulation of transcription in seed
    • Nakabayashi, K., Okamoto, M., Koshiba, T., Kamiya, Y. and Nambara, E. (2005) Genome-wide profiling of stored mRNA in Arabidopsis thaliana seed germination: epigenetic and genetic regulation of transcription in seed. Plant J. 41, 697-709.
    • (2005) Plant J. , vol.41 , pp. 697-709
    • Nakabayashi, K.1    Okamoto, M.2    Koshiba, T.3    Kamiya, Y.4    Nambara, E.5
  • 38
    • 0037356771 scopus 로고    scopus 로고
    • Correlated clustering and virtual display of gene expression patterns in the wheat life cycle by large-scale statistical analyses of expressed sequence tags
    • Ogihara, Y., Mochida, K., Nemoto, Y., Murai, K., Yamazaki, Y., Shin, I.T. and Kohara, Y. (2003) Correlated clustering and virtual display of gene expression patterns in the wheat life cycle by large-scale statistical analyses of expressed sequence tags. Plant J. 33, 1001-1011.
    • (2003) Plant J. , vol.33 , pp. 1001-1011
    • Ogihara, Y.1    Mochida, K.2    Nemoto, Y.3    Murai, K.4    Yamazaki, Y.5    Shin, I.T.6    Kohara, Y.7
  • 39
    • 0029170674 scopus 로고
    • The gene for the major cuticular wax-associated protein and three homologous genes from broccoli (Brassica oleracea) and their expression patterns
    • Pyee, J. and Kolattukudy, P. (1995) The gene for the major cuticular wax-associated protein and three homologous genes from broccoli (Brassica oleracea) and their expression patterns. Plant J. 49-59.
    • (1995) Plant J. , pp. 49-59
    • Pyee, J.1    Kolattukudy, P.2
  • 40
    • 0034119621 scopus 로고    scopus 로고
    • Heat shock protein 101 plays a crucial role in thermotolerance in Arabidopsis
    • Queitsch, C. Hong, S. Vierling, E. and Lindquist, S. (2000) Heat shock protein 101 plays a crucial role in thermotolerance in Arabidopsis. Plant Cell, 12, 479-492.
    • (2000) Plant Cell , vol.12 , pp. 479-492
    • Queitsch, C.1    Hong, S.2    Vierling, E.3    Lindquist, S.4
  • 41
    • 0141907260 scopus 로고    scopus 로고
    • Differential gene expression for suicide-substrate serine proteinase inhibitors (serpins) in vegetative and grain tissues of barley
    • Roberts, T.H. Marttila, S. Rasmussen, S.K. and Hejgaard, J. (2003) Differential gene expression for suicide-substrate serine proteinase inhibitors (serpins) in vegetative and grain tissues of barley. J. Exp. Bot. 54, 2251-2263.
    • (2003) J. Exp. Bot. , vol.54 , pp. 2251-2263
    • Roberts, T.H.1    Marttila, S.2    Rasmussen, S.K.3    Hejgaard, J.4
  • 42
    • 15444364810 scopus 로고    scopus 로고
    • Maximizing the efficacy of SAGE analysis identifies novel transcripts in Arabidopsis
    • Robinson, S. Cram, D. Lewis, C. and Parkin, I. (2004) Maximizing the efficacy of SAGE analysis identifies novel transcripts in Arabidopsis. Plant Physiol. 136, 3223-3233.
    • (2004) Plant Physiol. , vol.136 , pp. 3223-3233
    • Robinson, S.1    Cram, D.2    Lewis, C.3    Parkin, I.4
  • 43
    • 0034697796 scopus 로고    scopus 로고
    • Plant glycine-rich proteins: A family or just proteins with a common motif?
    • Sachetto-Martins, G. Franco, L. and de Oliveira, D. (2000) Plant glycine-rich proteins: a family or just proteins with a common motif? Biochim. Biophys. Acta. 1492, 1-14.
    • (2000) Biochim. Biophys. Acta. , vol.1492 , pp. 1-14
    • Sachetto-Martins, G.1    Franco, L.2    De Oliveira, D.3
  • 44
    • 0029110051 scopus 로고
    • Aluminum-induced genes (induction by toxic metals, low calcium, and wounding and pattern of expression in root tips)
    • Snowden, K. Richards, K. and Gardner, R. (1995) Aluminum-induced genes (induction by toxic metals, low calcium, and wounding and pattern of expression in root tips). Plant Physiol. 107, 341-348.
    • (1995) Plant Physiol. , vol.107 , pp. 341-348
    • Snowden, K.1    Richards, K.2    Gardner, R.3
  • 46
    • 1342311966 scopus 로고    scopus 로고
    • Transcript profiles and deduced changes of metabolic pathways in maternal and filial tissues of developing barley grains
    • Sreenivasulu, N. Altschmied, L. Radchuk, V. Gubatz, S. Wobus, U. and Weschke, W. (2004) Transcript profiles and deduced changes of metabolic pathways in maternal and filial tissues of developing barley grains. Plant J. 37, 539-553.
    • (2004) Plant J. , vol.37 , pp. 539-553
    • Sreenivasulu, N.1    Altschmied, L.2    Radchuk, V.3    Gubatz, S.4    Wobus, U.5    Weschke, W.6
  • 47
    • 0031801310 scopus 로고    scopus 로고
    • Differential regulation of the barley (Hordeum vulgare) transcripts B22E and B12D in mature aleurone layers
    • Steinum, T. Berner, H. Stacy, R. Salehian, Z. and Aalen, R. (1998) Differential regulation of the barley (Hordeum vulgare) transcripts B22E and B12D in mature aleurone layers. Physiol. Plant, 102, 337-345.
    • (1998) Physiol. Plant , vol.102 , pp. 337-345
    • Steinum, T.1    Berner, H.2    Stacy, R.3    Salehian, Z.4    Aalen, R.5
  • 48
    • 0037123632 scopus 로고    scopus 로고
    • An expanding universe of noncoding RNAs
    • Storz, G. (2002) An expanding universe of noncoding RNAs. Science, 296, 1260-1263.
    • (2002) Science , vol.296 , pp. 1260-1263
    • Storz, G.1
  • 49
    • 0000036537 scopus 로고
    • Isolation and analysis of the expression of two genes for the 81-kilodalton heat-shock proteins from Arabidopsis
    • Takahashi, T. Naito, S. and Yoshibumi, K. (1992) Isolation and analysis of the expression of two genes for the 81-kilodalton heat-shock proteins from Arabidopsis. Plant Physiol. 99, 383390.
    • (1992) Plant Physiol. , vol.99 , pp. 383390
    • Takahashi, T.1    Naito, S.2    Yoshibumi, K.3
  • 50
    • 0030573995 scopus 로고    scopus 로고
    • The effect of the length of the 3-untranslated region on expression in plants
    • Tanguay, R. and Gallie, D. (1996) The effect of the length of the 3-untranslated region on expression in plants. FEBS Lett. 394, 285-288.
    • (1996) FEBS Lett. , vol.394 , pp. 285-288
    • Tanguay, R.1    Gallie, D.2
  • 51
    • 0029655282 scopus 로고    scopus 로고
    • Translational efficiency is regulated by the length of the 3′ untranslated region
    • Tanguay, R. and Gallie, D. (1996a) Translational efficiency is regulated by the length of the 3′ untranslated region. Mol. Cell Biol. 16, 146-156.
    • (1996) Mol. Cell Biol. , vol.16 , pp. 146-156
    • Tanguay, R.1    Gallie, D.2
  • 52
    • 0034649566 scopus 로고    scopus 로고
    • Analysis of the genome sequence of the flowering plant
    • The Arabidopsis Genome Initiative
    • The Arabidopsis Genome Initiative. (2000) Analysis of the genome sequence of the flowering plant. Nature, 408, 796-815.
    • (2000) Nature , vol.408 , pp. 796-815
  • 56
    • 17444416148 scopus 로고    scopus 로고
    • LongSAGE analysis significantly improves genome annotation: Identifications of novel genes and alternative transcripts in the mouse
    • Wahl, M., Heinzmann, U. and Imai, K. (2005) LongSAGE analysis significantly improves genome annotation: identifications of novel genes and alternative transcripts in the mouse. Bioinformatics, 21, 1393-1400.
    • (2005) Bioinformatics , vol.21 , pp. 1393-1400
    • Wahl, M.1    Heinzmann, U.2    Imai, K.3
  • 57
    • 0032994572 scopus 로고    scopus 로고
    • Inventory of high-abundance mRNAs in skeletal muscle of normal men
    • Welle, S., Bhatt, K. and Thornton, C. (1999) Inventory of high-abundance mRNAs in skeletal muscle of normal men. Genome Res. 9, 506-513.
    • (1999) Genome Res. , vol.9 , pp. 506-513
    • Welle, S.1    Bhatt, K.2    Thornton, C.3
  • 61
    • 0030021536 scopus 로고    scopus 로고
    • Expression of a late embryogenesis abundent protein gene, HVA1, from barley confers tolerance to water deficit and salt in transgenic rice
    • Xu, D., Duan, X., Wang, B., Ho, T. and Wu, R. (1996) Expression of a late embryogenesis abundent protein gene, HVA1, from barley confers tolerance to water deficit and salt in transgenic rice. Plant Physiol. 110, 249-257.
    • (1996) Plant Physiol. , vol.110 , pp. 249-257
    • Xu, D.1    Duan, X.2    Wang, B.3    Ho, T.4    Wu, R.5
  • 62
    • 0035197071 scopus 로고    scopus 로고
    • Diversity of Arabidopsis genes encoding precursors for phytosulfokine, a peptide growth factor
    • Yang, H., Matsubayashi, Y., Nakamura, K. and Sakagami, Y. (2001) Diversity of Arabidopsis genes encoding precursors for phytosulfokine, a peptide growth factor. Plant Physiol. 127, 842-851.
    • (2001) Plant Physiol. , vol.127 , pp. 842-851
    • Yang, H.1    Matsubayashi, Y.2    Nakamura, K.3    Sakagami, Y.4
  • 63
    • 0037023384 scopus 로고    scopus 로고
    • A draft sequnce of the rice genome (Oryza sativa L. ssp. indica)
    • Yu, J. and Wang, J. (2002) A draft sequnce of the rice genome (Oryza sativa L. ssp. indica). Science, 296, 79-92.
    • (2002) Science , vol.296 , pp. 79-92
    • Yu, J.1    Wang, J.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.