Granulosa cell aromatase enzyme activity: Effects of follicular fluid from patients with polycystic ovary syndrome, using aromatase conversion and [ 11C]vorozole-binding assays

Gynecological Endocrinology

Volumn 22, Issue 12, 2006, Pages 685-691

  Kirilovas, Dmitrijus ^a   Chaika, Andrey ^b   Bergström, Mats ^a   Bergström Petterman, Elizabeth ^a   Carlström, Kjell ^c   Nosenko, Jelena ^b   Korniyenko, Svetlana ^b   Yakovets, Anna ^d   Mogilevkina, Iryna ^a,b   Naessen, Tord ^a  

^a UPPSALA UNIVERSITY HOSPITAL   (Sweden)

^b DONETSK MEDICAL UNIVERSITY   (Ukraine)

^c KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

^d Donetsk Regional Center of Mother and Child Care   (Ukraine)

Author keywords

11C vorozole; Aromatase; Follicular fluid; Inhibition; Polycystic ovary syndrome

Indexed keywords

AROMATASE; CARBON 11; CHARCOAL; ETHER; FOLLITROPIN; VORICONAZOLE;

ADULT; ARTICLE; BINDING AFFINITY; BINDING ASSAY; BINDING SITE; CELL CULTURE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME REGULATION; ENZYME SUBSTRATE; FEMALE; FERTILIZATION IN VITRO; GRANULOSA CELL; HUMAN; HUMAN CELL; INCUBATION TIME; OVARY FOLLICLE FLUID; OVARY POLYCYSTIC DISEASE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION;

AROMATASE; DIAGNOSTIC TECHNIQUES, RADIOISOTOPE; ESTROGENS; FEMALE; FOLLICULAR FLUID; GRANULOSA CELLS; HUMANS; POLYCYSTIC OVARY SYNDROME; SUBSTRATE SPECIFICITY; TRIAZOLES;

EID: 33845657388     PISSN: 09513590     EISSN: 14730766     Source Type: Journal    
DOI: 10.1080/09513590601037535     Document Type: Article

References (30)

1. McNatty KP, Smith DM, Makris A, Osathanondh R, Ryan KJ. The microenvironment of the human antral follicle: interrelationships among the steroid levels in antral fluid, the population of granulosa cells, and the status of the oocyte in vivo and in vitro. J Clin Endocrinol Metab 1979;49:851-860.
2. Gougeon A. Regulation of ovarian follicular development in primates: facts and hypotheses. Endocr Rev 1996;17:121-155.
3. Hillier SG, Reichert LE Jr, Van Hall EV. Control of preovulatory follicular estrogen biosynthesis in the human ovary. J Clin Endocrinol Metab 1981;52:847-856.
4. Hillier SG. Current concepts of the roles of follicle stimulating hormone and luteinizing hormone in folliculogenesis. Hum Reprod 1994;9:188-191.
5. Weil S, Vendola K, Zhou J, Bondy CA. Androgen and follicle-stimulating hormone interactions in primate ovarian follicle development. J Clin Endocrinol Metab 1999;84:2951-2956.
6. Ledwitz-Rigby F. Follicular fluid stimulation of estrogen secretion by immature porcine granulosa cells. Mol Cell Endocrinol 1983;29:213-222.
7. diZerega GS, Goembelsmann U, Nakamura RM. Identification of protein(s) secreted by the preovulatory ovary which suppresses the follicle response to gonadotropins. J Clin Endocrinol Metab 1982;54:1091-1096.
8. diZerega GS, Campeau JD, Nakamura RN, Ujita EL, Lobo R, Marrs RP. Activity of a human follicular fluid protein(s) in spontaneous and induced ovarian cycles. J Clin Endocrinol Metab 1983;57:838-846.
9. diZerega GS, Marrs RP, Campeau JD, Kling OR. Human granulosa cell secretion of protein(s) which suppress follicular response to gonadotropins. J Clin Endocrinol Metab 1983;56: 147-155.
10. Franks S. Polycystic ovary syndrome: a changing perspective. Clin Endocrinol (Oxf) 1989;31:87-120.
11. Cataldo NA, Giudice LC. Follicular fluid insulin-like growth factor binding protein profiles in polycystic ovary syndrome. J Clin Endocrinol Metab 1992;74:695-697.
12. Guet P, Royere D, Paris A, Lansac J, Driancourt MA. Aromatase activity of human granulosa cells in vitro: effects of gonadotrophins and follicular fluid. Hum Reprod 1999;14: 1182-1189.
13. Agarwal SK, Judd HL, Magoffin DA. A mechanism for the suppression of estrogen production in polycystic ovary syndrome. J Clin Endocrinol Metab 1996;81:3686-3691.
14. Wouters W, Snoeck E, De Coster R. Vorozole, a specific nonsteroidal aromatase inhibitor. Breast Cancer Res Treat 1994; 30:89-94.
15. de Jong PC, van de Ven J, Nortier HW, Maitimu-Smeele I, Donker TH, Thijssen JH, Slee PH, Blankenstein RA. Inhibition of breast cancer tissue aromatase activity and estrogen concentrations by the third-generation aromatase inhibitor vorozole. Cancer Res 1997;57:2109-2111.
16. 11C]vorozole. Nucl Med Biol 1998;25:497-501.
17. 11C]vorozole binding assay. Steroids 1999;64:266-272.
18. Adams J, Polson DW, Franks S. Prevalence of polycystic ovaries in women with anovulation and idiopathic hirsutism. Br Med J (Clin Res Ed) 1986;293:355-359.
19. Ferriman D, Gallwey G. Clinical assessment of body hair growth in women. J Clin Endocrinol Metab 1961;21:1440-1447.
20. Newton CJ, Samuel DL, James VH. Aromatase activity and concentrations of cortisol, progesterone and testosterone in breast and abdominal adipose tissue. J Steroid Biochem 1986; 24:1033-1039.
21. Johnston RF, Pickett SC, Barker DL. Autoradiography using storage phosphor technology. Electrophoresis 1990;11:355-360.
22. 11C-vorozole binding in granulosa cells in vitro. Acta Obstet Gynecol Scand 2002;82:209-215.
23. 11C]vorozole binding in ovarian tissue in rats throughout estrous cycle in association with conversion of androgens to estrogens in vivo and in vitro. Steroids 2003;68:1139-1146.
24. McNatty KP, Baird DT. Relationship between folliclestimulating hormone, androstenedione and oestradiol in human follicular fluid. J Endocrinol 1978;76:527-531.
25. Hillier SG, van den Boogaard AM, Reichert LE Jr, van Hall EV. Intraovarian sex steroid hormone interactions and the regulation of follicular maturation: aromatization of androgens by human granulosa cells in vitro. J Clin Endocrinol Metab 1980;50:640-647.
26. Hillier SG, De Zwart FA. Evidence that granulosa cell aromatase induction/activation by follicle-stimulating hormone is an androgen receptor-regulated process in-vitro. Endocrinology 1981;109:1303-1305.
27. Westergaard L, Christensen IJ, McNatty KP. Steroid levels in ovarian follicular fluid related to follicle size and health status during the normal menstrual cycle in women. Hum Reprod 1986;1:227-232.
28. Uzumcu M, Lin YC. Characterization of the stimulatory actions of thymic factor(s) on basal and gonadotropin-induced steroidogenesis in cultured rat granulosa cells. Mol Cell Endocrinol 1994;105:209-216.
29. Davoren JB, Hsueh AJ. Vasoactive intestinal peptide: a novel stimulator of steroidogenesis by cultured rat granulosa cells. Biol Reprod 1985;33:37-52.
30. Saragueta P, Krimer AR, Charreau EH, Tesone M. Insulin regulation of steroidogenic activity in rat culture luteal cells. J Steroid Biochem 1989;32:393-397.