Airway epithelial (nasal) cell monolayers used to study Pseudomonas aeruginosa invasion are hyperpolarized and not representative of the human airway epithelium [1]

Infection and Immunity

Volumn 74, Issue 12, 2006, Pages 7043-

  Pier, Gerald B ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RHAMNOLIPID; TRANSMEMBRANE CONDUCTANCE REGULATOR;

AIRWAY RESISTANCE; BACTERIAL VIRULENCE; CELL INVASION; CELL POLARITY; CYSTIC FIBROSIS; ENDOCYTOSIS; HUMAN; HYPERPOLARIZATION; KNOCKOUT MOUSE; LETTER; LUNG FIBROSIS; MONKEY; MONOLAYER CULTURE; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; RESPIRATORY EPITHELIUM; TRANSEPITHELIAL RESISTANCE;

ANIMALS; ARTIFACTS; CELL POLARITY; CELLS, CULTURED; HUMANS; MODELS, BIOLOGICAL; NASAL MUCOSA; PSEUDOMONAS AERUGINOSA;

EID: 33845512285     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.01233-06     Document Type: Letter

References (5)

1. Barker, P. M., R. C. Boucher, and J. R. Yankaskas. 1995. Bioelectric properties of cultured monolayers from epithelium of distal human fetal lung. Am. J. Physiol. 268:L270-L277.
2. Boucher, R. C., M. J. Stutts, M. R. Knowles, L. Cantley, and J. T. Gatzy. 1986. Na+ transport in cystic fibrosis respiratory epithelia. Abnormal basal rate and response to adenylate cyclase activation. J. Clin. Investig. 78:1245-1252.
3. Coyne, C. B., T. M. Gambling, R. C. Boucher, J. L. Carson, and L. G. Johnson. 2003. Role of claudin interactions in airway tight junctional permeability. Am. J. Physiol. Lung Cell Mol. Physiol. 285:L1166-1178.
4. Schroeder, T. H., N. Reiniger, G. Meluleni, M. Grout, F. T. Coleman, and G. B. Pier. 2001. Transgenic cystic fibrosis mice exhibit reduced early clearance of Pseudomonas aeruginosa from the respiratory tract. J. Immunol. 166:7410-7418.
5. Zulianello, L., C. Canard, T. Kohler, D. Caille, J. S. Lacroix, and P. Meda. 2006. Rhamnolipids are virulence factors that promote early infiltration of primary human airway epithelia by Pseudomonas aeruginosa. Infect. Immun. 74:3134-3147.