HuGE, a novel GFP-actin-expressing mouse line for studying cytoskeletal dynamics

European Journal of Cell Biology

Volumn 86, Issue 1, 2007, Pages 3-12

  Gurniak, Christine B ^a   Witke, Walter ^a  

^a EUROPEAN MOLECULAR BIOLOGY LABORATORY EMBL   (Italy)

Author keywords

Actin dynamics; GFP actin; Lymphocytes; Profilin 1

Indexed keywords

CYTOSKELETON PROTEIN; F ACTIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROFILIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BLOOD CELL; BONE MARROW CELL; CONTROLLED STUDY; EMBRYO; EMBRYO DEVELOPMENT; GENE EXPRESSION; GENE LOCUS; MOLECULAR DYNAMICS; MOUSE; MOUSE STRAIN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN STRUCTURE; SKELETAL MUSCLE;

EID: 33845503108     PISSN: 01719335     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ejcb.2006.08.007     Document Type: Article

References (24)

1. Azuma T., Witke W., Stossel T.P., Hartwig J.H., and Kwiatkowski D.J. Gelsolin is a downstream effector of rac for fibroblast motility. EMBO J. 17 (1998) 1362-1370
2. Bettinger B.T., Gilbert D.M., and Amberg D.C. Actin up in the nucleus. Nat. Rev. Mol. Cell Biol. 5 (2004) 410-415
3. Bohnsack M.T., Stuven T., Kuhn C., Cordes V.C., and Gorlich D. A selective block of nuclear actin export stabilizes the giant nuclei of Xenopus oocytes. Nat. Cell Biol. 8 (2006) 257-263
4. Choidas A., Jungbluth A., Sechi A., Murphy J., Ullrich A., and Marriott G. The suitability and application of a GFP-actin fusion protein for long-term imaging of the organization and dynamics of the cytoskeleton in mammalian cells. Eur. J. Cell Biol. 77 (1998) 81-90
5. Di Nardo A., Gareus R., Kwiatkowski D., and Witke W. Alternative splicing of the mouse profilin II gene generates functionally different profilin isoforms. J. Cell Sci. 113 (2000) 3795-3803
6. Dotti C.G., Sullivan C.A., and Banker G.A. The establishment of polarity by hippocampal neurons in culture. J. Neurosci. 8 (1988) 1454-1468
7. Fischer M., Kaech S., Wagner U., Brinkhaus H., and Matus A. Glutamate receptors regulate actin-based plasticity in dendritic spines. Nat. Neurosci. 3 (2000) 887-894
8. Gerisch G., and Weber I. Cytokinesis without myosin II. Curr. Opin. Cell Biol. 12 (2000) 126-132
9. Giesemann T., Rathke-Hartlieb S., Rothkegel M., Bartsch J.W., Buchmeier S., Jockusch B.M., and Jockusch H. A role for polyproline motifs in the spinal muscular atrophy protein SMN. Profilins bind to and colocalize with smn in nuclear gems. J. Biol. Chem. 274 (1999) 37908-37914
10. Gonsior S.M., Platz S., Buchmeier S., Scheer U., Jockusch B.M., and Hinssen H. Conformational difference between nuclear and cytoplasmic actin as detected by a monoclonal antibody. J. Cell Sci. 112 (1999) 797-809
11. Huisken J., Swoger J., Del Bene F., Wittbrodt J., and Stelzer E.H. Optical sectioning deep inside live embryos by selective plane illumination microscopy. Science 305 (2004) 1007-1009
12. Lenart P., Bacher C.P., Daigle N., Hand A.R., Eils R., Terasaki M., and Ellenberg J. A contractile nuclear actin network drives chromosome congression in oocytes. Nature 436 (2005) 812-818
13. McDonald D., Carrero G., Andrin C., de Vries G., and Hendzel M.J. Nucleoplasmic beta-actin exists in a dynamic equilibrium between low-mobility polymeric species and rapidly diffusing populations. J. Cell Biol. 172 (2006) 541-552
14. Ponte P., Ng S.Y., Engel J., Gunning P., and Kedes L. Evolutionary conservation in the untranslated regions of actin mRNAs: DNA sequence of a human beta-actin cDNA. Nucleic Acids Res. 12 (1984) 1687-1696
15. Racoosin E.L., and Swanson J.A. Macrophage colony-stimulating factor (rM-CSF) stimulates pinocytosis in bone marrow-derived macrophages. J. Exp. Med. 170 (1989) 1635-1648
16. Sanger J.W., Sanger J.M., Kreis T.E., and Jockusch B.M. Reversible translocation of cytoplasmic actin into the nucleus caused by dimethyl sulfoxide. Proc. Natl. Acad. Sci. USA 77 (1980) 5268-5272
17. Schwenk F., Baron U., and Rajewsky K. A cre-transgenic mouse strain for the ubiquitous deletion of loxP-flanked gene segments including deletion in germ cells. Nucleic Acids Res. 23 (1995) 5080-5081
18. Theriot J.A., Mitchison T.J., Tilney L.G., and Portnoy D.A. The rate of actin-based motility of intracellular Listeria monocytogenes equals the rate of actin polymerization. Nature 357 (1992) 257-260
19. Trifaro J.M., Lejen T., Rose S.D., Pene T.D., Barkar N.D., and Seward E.P. Pathways that control cortical F-actin dynamics during secretion. Neurochem. Res. 27 (2002) 1371-1385
20. Vaezi A., Bauer C., Vasioukhin V., and Fuchs E. Actin cable dynamics and Rho/Rock orchestrate a polarized cytoskeletal architecture in the early steps of assembling a stratified epithelium. Dev. Cell 3 (2002) 367-381
21. Westphal M., Jungbluth A., Heidecker M., Muhlbauer B., Heizer C., Schwartz J.M., Marriott G., and Gerisch G. Microfilament dynamics during cell movement and chemotaxis monitored using a GFP-actin fusion protein. Curr. Biol. 7 (1997) 176-183
22. Witke W., Sharpe A.H., Hartwig J.H., Azuma T., Stossel T.P., and Kwiatkowski D.J. Hemostatic, inflammatory, and fibroblast responses are blunted in mice lacking gelsolin. Cell 81 (1995) 41-51
23. Witke W., Sutherland J.D., Sharpe A., Arai M., and Kwiatkowski D.J. Profilin I is essential for cell survival and cell division in early mouse development. Proc. Natl. Acad. Sci. USA 98 (2001) 3832-3836
24. Yarar D., Waterman-Storer C.M., and Schmid S.L. A dynamic actin cytoskeleton functions at multiple stages of clathrin-mediated endocytosis. Mol. Biol. Cell 16 (2005) 964-975