Cloning and functional expression of an acyl-ACP thioesterase FatB type from Diploknema (Madhuca) butyracea seeds in Escherichia coli

Plant Physiology and Biochemistry

Volumn 44, Issue 11-12, 2006, Pages 645-655

  Jha, J K ^a   Maiti, M K ^a   Bhattacharjee, A ^a   Basu, A ^a   Sen, P C ^b   Sen, S K ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY   (India)

^b BOSE INSTITUTE   (India)

Author keywords

Acyl acyl carrier protein (ACP) thioesterase; Diploknema (Madhuca) butyracea; FatB cDNA; Fatty acid profile analysis; Heterologous expression in E. coli

Indexed keywords

BACTERIA (MICROORGANISMS); BRASSICA; BRASSICA JUNCEA; DIPLOKNEMA BUTYRACEA; ESCHERICHIA COLI; MADHUCA;

RECOMBINANT PROTEIN; THIOL ESTER HYDROLASE; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENETICS; GENOME; MADHUCA; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PLANT SEED;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION; GENOME, PLANT; MADHUCA; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; RECOMBINANT PROTEINS; SEEDS; THIOLESTER HYDROLASES;

EID: 33845475438     PISSN: 09819428     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plaphy.2006.09.017     Document Type: Article

References (34)

1. + RNA, in. Methods Enzymol. 152 (1987) 254-261
2. Bhattacharjee A., Ghosh S.K., Ghosh D., Ghosh S., Maiti M.K., and Sen S.K. Identification of a heat-stable palmitoyl/oleoyl acyl-acyl carrier protein thioesterase in developing seeds of Madhuca butyracea. Plant Sci. 163 4 (2002) 791-800
3. Bligh E.G., and Dyer W.J. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37 (1959) 911-917
4. Bonaventure G., Salas J.J., Pollard M.R., and Ohlrogge J.B. Disruption of FATB gene in Arabidopsis demonstrates an essential role of saturated fatty acids in plant growth. Plant Cell 15 (2003) 1020-1033
5. Combet C., Blanchet C., Geourjon C., and Deléage G. NPS@: Network Protein Sequence Analysis. Trends Biochem. Sci. 25 3 (2000) 147-150 [291]
6. Davies H.M. Medium chain acyl-ACP hydrolysis activities of developing oilseeds. Phytochemistry 33 (1993) 1353-1356
7. Davies H.M., Anderson L., Fan C., and Hawkins D.J. Developmental induction, purification, and further characterization of C12:0-ACP thioesterase from immature cotyledons of Umbellularia californica. Arch. Biochem. Biophys. 290 (1991) 37-45
8. Dehesh K., Jones A., Knutzon D.S., and Volelker T.A. Production of high levels of 8:0 and 10:0 fatty acids in transgenic canola by over expression of ChFatB2, a thioesterase cDNA from Cuphea hookeriana. Plant J. 9 (1996) 167-172
9. Dörmann P., Spener F., and Ohlrogge J.B. Characterization of two acyl-acyl carrier protein thioesterases from developing Cuphea seeds specific for medium-chain and oleoyl-acyl carrier protein. Planta 189 (1993) 425-432
10. Dörmann P., Voelker T.A., and Ohlrogge J.B. Cloning and expression in Escherichia coli of a novel thioesterase from Arabidopsis thaliana specific for long-chain acyl-acyl carrier proteins. Arch. Biochem. Biophys. 316 (1995) 612-618
11. Dormann P., Voelker T.A., and Ohlrogge J.B. Accumulation of palmitate in Arabidopsis mediated by acyl-acyl carrier protein thioesterase FATB1. Plant Physiol. 123 (2000) 637-643
12. Doyle J.J., and Doyle J.L. Isolation of plant DNA from fresh tissue. BRLFocus 12 (1990) 13-15
13. Facciotti M.T., Bertain P.B., and Yaun L. Improved stearate phenotype in transgenic canola expressing a modified acyl-acyl carrier protein thioesterase. Nat. Biotechnol. 17 (1999) 593-597
14. Hawkins D.J., and Kridl J.C. Characterization of acyl-ACP thioesterase of mangosteen (Garcinia mangostana) seed and high level of stearate production in transgenic canola. Plant J. 13 (1998) 743-752
15. Hills M.J. Improving oil functionality by tuning catalysis of thioesterase. Trends Plant Sci. 4 (1999) 421-422
16. Huynh T.T., Pirtle R.M., and Chapman K.D. Expression of a Gossypium hirsutum cDNA encoding a FatB palmitoyl-acyl carrier protein thioesterase in Escherichia coli. Plant Physiol. Biochem. 40 (2002) 1-9
17. Jones A., Davies H.M., and Voelker T.A. Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases. Plant Cell 7 (1995) 359-371
18. Kimberly M.M., and Shanklin J. A structural model of the plant acyl-acyl carrier protein thioesterase FatB comprises two helix/4-stranded sheet domains, the N-terminal domain containing residues that affect specificity and the C-terminal domain containing catalytic residues. J. Biol. Chem. 280 (2005) 3621-3627
19. Loader N.M., Woolner E.M., Hellyer A., Salbas A.R., and Safford R. Isolation and characterization of two Brassica napus embryo acyl-ACP thioesterase cDNA clones. Plant Mol. Biol. 23 (1993) 769-778
20. Marr A.G., and Ingraham J.L. Effect of temperature on the composition of fatty acids in Escherichia coli. J. Bacteriol. 84 (1962) 1260-1267
21. Overath P., Pauli G., and Schairer H.U. Fatty acid degradation in Escherichia coli: an inducible acyl-CoA synthetase, the mapping of old mutations, and the isolation of regulatory mutants. Eur. J. Biochem. 7 (1969) 559-574
22. Pathak M.K., Bhattacharjee A., Ghosh D., and Ghosh S. Acyl-acyl carrier protein (ACP)-thioesterase from developing seeds of Brassica campestris cv. B-54 (Agrani). Plant Sci. 166 (2004) 191-198
23. Salas J.J., and Ohlrogge J.B. Characterization of substrate specificity of FatA and FatB acyl-ACP thioesterases. Arch. Biochem. Biophys. 403 (2002) 25-34
24. Sambrook J., Fritsch E.F., and Maniatis T. Molecular Cloning: A Laboratory Manual, second ed (1989), Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
25. Sengupta A., and Roy Choudhury S.K. Triglyceride composition of Madhuca butyracea seed fat. J. Am. Oil Chem. Soc. 55 (1978) 621-624
26. Serrano-Vega M.J., Garcés R., and Martinez-Force E. Cloning, characterization and structural model of a FatA-type thioesterase from sunflower seeds (Helianthus annuus L.). Planta 221 (2005) 868-880
27. Serrano-Vega M.J., Venegas-Calerón M., Garcés R., and Martinez-Force E. Cloning and expression of fatty acids biosynthesis key enzymes from sunflower (Helianthus annuus L.) in Escherichia coli. J. Chromatogr. B. 786 (2003) 221-228
28. Verwoerd T.C., Dekker B.M.M., and Hoekema A. A small-scale procedure for the rapid isolation of plant RNAs. Nucleic Acids Res. 17 (1989) 2362
29. Villaverde A., and Carrio M.M. Protein aggregation in recombinant bacteria: biological role of inclusion bodies. Biotechnol. Lett. 25 (2003) 1385-1395
30. Voelker T.A., and Davies H.M. Alteration of the specificity and regulation of fatty acid synthesis of Escherichia coli by expression of a plant medium-chain acyl-acyl carrier protein thioesterase. J. Bacteriol. 176 (1994) 7320-7327
31. Voelker T.A., Jones A., Cranmer A.M., Davies H.M., and Knutzon D.S. Broad-range and binary-range acyl-acyl carrier protein thioesterase suggest an alternative mechanism for medium chain production in seeds. Plant Physiol. 114 (1997) 669-677
32. Woestenenk E.A., Hammarström M., van den Berg S., Härd T., and Berglund H. His tag effect on solubility of human proteins produced in Escherichia coli: a comparison between four expression vectors. J. Struct. Funct. Genomics 5 (2004) 217-229
33. Yuan L., Nelson B.A., and Caryl G. The catalytic cysteine and histidine in the plant acyl-acyl carrier protein thioesterases. J. Biol. Chem. 271 (1996) 3417-3419
34. Yuan L., Voelker T.A., and Hawkins D.J. Modification of substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering. Proc. Natl. Acad. Sci. USA 92 (1995) 10639-10643