Improved stearate phenotype in transgenic canola expressing a modified acyl-acyl carrier protein thioesterase

Nature Biotechnology

Volumn 17, Issue 6, 1999, Pages 593-597

  Facciotti, Marc T ^b   Bertain, Paul B ^a   Yuan, Ling ^c  

^a Calgene LLC   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c Maxygen   (United States)

Author keywords

Alanine scanning; Protein engineering; Site directed mutagenesis; Stearate; Transgenic plants

Indexed keywords

ACYL CARRIER PROTEIN; STEARIC ACID; THIOL ESTER HYDROLASE;

ARTICLE; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME ISOLATION; FATTY ACID METABOLISM; GENE EXPRESSION; PHENOTYPE; PLANT; PRIORITY JOURNAL; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; BRASSICA; DNA PRIMERS; MOLECULAR SEQUENCE DATA; MUTATION; PHENOTYPE; PLANTS, GENETICALLY MODIFIED; SEQUENCE HOMOLOGY, AMINO ACID; STEARIC ACIDS; THIOLESTER HYDROLASES;

EID: 0033028744     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/9909     Document Type: Article

References (21)

1. Nelson, G. Dietary fat, Trans fatty acids, and risk of coronary heart disease Nutr. Rev. 56, 250-252 (1998).
2. Katan, M.B., Zock, P.L. & Mensink, R.P. Trans fatty acids and their effects on lipoproteins in humans. Annu. Rev. Nutr. 15, 473-493 (1995).
3. Yuan, L. & Knauf, V.C. Modification of plant components. Curr. Opin. Biotechnol. 8, 227-233 (1997).
4. Del Vecchio, A.J. High-laurate canola. International News on Fats, Oils and Related Materials 7, 230-243 (1996).
5. Hawkins, D. & Kridl, J. Characterization of acyl-ACP thioesterases of mangosteen (Garcinia mangostana) seed and high levels of stearate production in transgenic canola. Plant J. 13, 743-752 (1998).
6. Jones, A., Davies, H.M. & Volker, T.A. Palmitoyl-acyl carrier protein (ACP) thioesterase and the evolutionary origin of plant acyl-ACP thioesterases. Plant Cell 7, 359-371 (1995).
7. Volker, T, in Genetic engineering, (ed. Setlow, J.K.) 111-133 (Plenum, New York; 1996).
8. Facciotti, M. & Yuan, L. Molecular dissection of the plant acyl-acyl carrier protein thioesterases. FETT/Lipid 100(4-5), 167-172 (1998).
9. Yuan, L., Voelker, T.A. & Hawkins, D.J. Modification of the substrate specificity of an acyl-acyl carrier protein thioesterase by protein engineering. Proc. Natl. Acad. Sci. USA 92, 10639-10643 (1995).
10. Yuan, L., Nelson, B.A. & Caryl, G. The catalytic cysteine and histidine in the plant acyl-acyl carrier protein thioesterases. J. Biol. Chem. 271, 3417-3419 (1996).
11. Nickerson, D.P., Harford-Cross, C.F., Fulcher, S.R. & Wong, L-L. The catalytic activity of cytochrome P450cam towards styrene oxidation is increased by site-specific mutagenesis. FEBS Lett. 405, 153-156 (1997).
12. Gibbs, C. & Zoller, M. Rational scanning mutagenesis of a protein kinase identifies functional regions involved in catalysis and substrate interactions. J. Biol. Chem. 266, 8923-8931 (1991).
13. Kristensen, C. et al. Alanine scanning mutagenesis of insulin. J. Biol. Chem. 272, 12978-12983 (1997).
14. Cahoon, E.B., Lindqvist, Y., Schneider, G. & Shanklin, J. Redesign of soluble fatty acid desaturases from plants for altered substrate specificity and double bond position. Proc. Natl. Acad. Sci. USA 94, 4872-4877 (1997).
15. Knudson D.S. et al Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. Proc. Natl. Acad. Sci. USA 89, 2624-2628 (1992).
16. McBride, K. E. & Summerfelt. K.R. Improved binary vectors for Agrobacterium-mediated plant transformation. Plant Mol. Biol. 14, 269-276 (1990).
17. Radke, S.E., Turner, J.C. & Facciotti, D. Transformation and regeneration of Brassica rapa using Agrobacterium tumefaciens. Plant Cell Rep. 11, 499-505 (1992).
18. Davies, K.M., Andereon, L., Fan, C. & Hawkins, D.J. Developmental induction, purification, and further characterization of 12:0-ACP thioesterase from immature cotyledons of Umbellularia californica. Arch. Biochem. Biophys. 290, 37-45 (1991).
19. Browse, J., McCourt, P.J. & Somerville, C.R. Fatty acid composition of leaf lipids determined after combined digestion and fatty acid methyl ester formation from fresh tissue. Anal. Biochem. 152, 141-145 (1986).
20. Bernatzky, R. & Tanksley, S.D. Genetics of actin-related sequences in tomato. Theor. Appl. Genet. 72, 314-321 (1986).
21. Maniatis, T., Fritsch, E.F. & Sambrook. J. Molecular cloning: a laboratory manual. (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1982).