Atomic force microscope visualization of lipid bilayer degradation due to action of phospholipase A2 and Humicola lanuginosa lipase

Biochimica et Biophysica Acta - Biomembranes

Volumn 1768, Issue 1, 2007, Pages 90-99

  Balashev, Konstantin ^a,b,d   John DiNardo, N ^b,c   Callisen, Thomas H ^c   Svendsen, Allan ^c   Bjørnholm, Thomas ^d  

^a UNIVERSITY OF SOFIA   (Bulgaria)

^b DREXEL UNIVERSITY   (United States)

^c NOVOZYMES A S   (Denmark)

^d UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Atomic force microscopy; Enzyme kinetics; Humicola lanuginose lipase; Phospholipase A2; Supported bilayers

Indexed keywords

PHOSPHOLIPASE A2; TRIACYLGLYCEROL LIPASE;

ARTICLE; ATOMIC FORCE MICROSCOPY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME STRUCTURE; FUNGUS; HUMICOLA LANUGINOSA; LANGMUIR BLODGETT FILM; LIPID BILAYER; LIPID DEGRADATION; LIPOLYSIS; MICROSCOPE; PRIORITY JOURNAL;

1,2-DIPALMITOYLPHOSPHATIDYLCHOLINE; CROTALID VENOMS; DIGLYCERIDES; FUNGI; HYDROLYSIS; KINETICS; LIPASE; LIPID BILAYERS; LIPOLYSIS; MICROSCOPY, ATOMIC FORCE; MOLECULAR CONFORMATION; PHOSPHOLIPASES A;

HUMICOLA; THERMOMYCES LANUGINOSUS;

EID: 33845465498     PISSN: 00052736     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbamem.2006.09.028     Document Type: Article

References (37)

1. Binnig G., Quate C.F., and Gerber C. Atomic force microscope. Phys. Rev. Lett. 56 (1986) 930-933
2. Prater C.B., Butt H.J., and Hansma P.K. Atomic force microscopy. Nature 345 (1990) 839-840
3. Hansma H.G. Surface biology of DNA by atomic force microscopy. Annu. Rev. Phys. Chem. 52 (2001) 71-92
4. Lin H., Clegg D.O., and Lal R. Imaging real-time proteolysis of single collagen I molecules with an atomic force microscope. Biochemistry-US 38 (1999) 9956-9963
5. Radmacher M., Fritz M., Hansma H.G., and Hansma P.K. Direct observation of enzyme activity with the atomic-force microscope. Science 265 (1994) 1577-1579
6. Grandbois M., Clausen-Schaumann H., and Gaub H.E. Atomic force microscope imaging of phospholipid bilayer degradation by phospholipase A(2). Biophys. J. 74 (1998) 2398-2404
7. Nielsen L.K., Risbo J., Callisen T.H., and Bjørnholm T. Lag-burst kinetics in phospholipase A(2) hydrolysis of DPPC bilayers visualized by atomic force microscopy. BBA-Biomembr. Acta 1420 (1998) 266-271
8. Nielsen L.K., Balashev K., Callisen T.H., and Bjørnholm T. Influence of product phase separation on phospholipase A2 hydrolysis of supported phospholipid bilayers studied by force microscopy. Biophys. J. 83 (2002) 2617-2624
9. Balashev K., Gudmand M., Iversen L., Callisen T.H., Svendsen A., and Bjørnholm T. Humicola lanuginosa lipase hydrolysis of mono-oleoyl-rac-glycerol at the lipid-water interface observed by atomic force microscopy. BBA-Biomembranes 1615 (2003) 93-102
10. Clausen-Schaumann H., Grandbois M., and Gaub H.E. Enzyme-assisted nanoscale lithography in lipid membranes. Adv. Mater. 10 12 (1999) 49
11. Verger R., and deHaas G.H. Interfacial enzyme-kinetics of lipolysis. Annu. Rev. Biophys. Bio. 5 (1976) 77-117
12. Gelb M.H., Jain M.K., Hanel A.M., and Berg O.G. Interfacial enzymology of glycerolipid hydrolases: lessons from secreted phospholipases A2. Annu. Rev. Biochem. 64 (1995) 653-688
13. Waite M. The Phospholipases (1987), Plenum Press, New York
14. Panaiotov I., and Verger R. In: Bazkin A.W.N.W. (Ed). Physical Chemistry of Biological Interfaces (2000), Marcel Dekker, New York 359-400
15. Ransac S., Moreau H., Riviere C., and Verger R. Monolayer techniques for studying phospholipase kinetics. Method Enzymol. 197 (1991) 49-65
16. Chen S., and Abruna H.D. Enzymatic activity of a Phospholipase A2: an electrochemical approach. Langmuir 13 (1997) 5969-5973
17. Jain M.K., and Maliwal B.P. Spectroscopic properties of the states of pig pancreatic phospholipase A2 at interfaces and their possible molecular origin. Biochemistry-US 32 (1993) 11838-11846
18. Grainger D.W., Reichert A., Ringsdorf H., and Salesse C. Hydrolytic action of phospholipase A2 in monolayer phase transition region: direct observation of enzyme domain formation using fluorescence microscopy. BBA-Biomembranes 1023 (1990) 365-379
19. Apitz-Castro R., Jain M.K., and De Haas G.H. Origin of the latency phase during the action of phospholipase A2 on unmodified phosphatidylcholine vesicles. BBA-Biomembranes 688 (1982) 349-356
20. Lawson D.M., Brzozowski A.M., Dodson G.G., Hubbard R.E., Huge-Jensen B., Boel E., and Derewenda Z.S. In: Woolley P., and Petersen S.B. (Eds). Lipases; Their Structure, Biochemistry and Application (1994), Cambridge Univ. Press, Cambridge 77-95
21. Thirstrup K., Verger R., and Carriere F. Evidence for a pancreatic lipase subfamily with new kinetic-properties. Biochemistry-US 33 (1994) 2748-2756
22. Berg O.G., Cajal Y., Butterfoss G.L., Grey R.L., Alsina M.A., Yu B.Z., and Jain M.K. Interfacial activation of triglyceride lipase from thermomyces (Humicola) lanuginosa: kinetic parameters and a basis for control of the lid. Biochemistry-US 37 (1998) 6615-6627
23. Verger R., and deHaas G.H. Enzyme reactions in a membrane model: 1. New technique to study enzyme reactions in monolayers. Chem. Phys. Lipids 10 (1973) 127-136
24. Brockman H.L., Kezdy F.J., and Law J.H. Isobaric titration of reacting monolayers-kinetics of hydrolysis of glycerides by pancreatic lipase-B. J. Lipid Res. 16 (1975) 67-74
25. Momsen W.E., and Brockman H.L. Recovery of monomolecular films in studies of lipolysis. Method Enzymol. 286 (1997) 292-305
26. Gargouri Y., Moreau H., and Verger R. Gastric lipases: biochemical and physiological studies. BBA-Lipid Lipid Met. 1006 3 (1989) 255-271
27. Maloney K.M., and Grainger D.W. Phase separated anionic domains in ternary mixed lipid monolayers at the air-water interface. Chem. Phys. Lipids 65 (1993) 31-42
28. Shao Z., and Yang J. Progress in high resolution atomic force microscopy in biology. Q. Rev. Biophys. 28 (1995) 195-251
29. Shao Z., Mou J., Czajkowsky D.M., Yang J., and Yuan J.Y. Biological atomic force microscopy: what is achieved and what is needed. Adv. Phys. 45 (1996) 1-86
30. Jensen T.R., and Kjaer K. In: Mobius D., and Miller R. (Eds). Novel Methods to Study Interfacial Layers (2001), Elsevier, New York 205-254
31. Ding J., Takamoto D.Y., von Nahmen A., Lipp M.M., Lee K.Y.C., Waring A.J., and Zasadzinski J.A. Effects of lung surfactant proteins, SP-B and SP-C, and palmitic acid on monolayer stability. Biophys. J. 80 (2001) 2262-2272
32. Riechert A., Ringsdorf H., and Wagenknecht A. Spontaneous domain formation of phospholipase A2 at interfaces: fluorescence microscopy of the interaction of phospholipase A2 with mixed monolayers of lecithin, lysolecithin and fatty acid. BBA-Biomembranes 1106 (1992) 178-188
33. Gelb M.H., Min J.H., and Jain M.K. Do membrane bound enzymes access their substrates from the membrane or aqueous phase: interfacial versus noninterfacial enzymes. BBA-Mol. Cell. Biol. L. 1488 (2000) 20-27
34. Yapoudjian S., Ivanova M., Douchet I., Zenatti A., Sentis M., Marine W., Svendsen A., and Verger R. Surface fluorescence resonance energy transfer studies on interfacial adsorption of thermomyces (Humicola) lanuginosa lipase, using monomolecular films of cis-parinaric acid. Biopolymers 65 2 (2002) 121-128
35. Pieroni G., Gargouri Y., Sarda L., and Verger R. Interactions of lipases with lipid monolayers. Facts and fictions. Adv. Colloid Interface 32 (1990) 341-378
36. Brockman H.L. Kinetic behavior of the pancreatic lipase-colipase-lipid system. Biochimie 82 (2000) 987-995
37. Tsujita T., Muderhwa J.M., and Brockman H.L. Lipid-lipid interactions as regulators of carboxylester lipase activity. J. Biol. Chem. 264 (1989) 8612-8618