Intrinsic activity of inulinase From Kluyveromyces marxianus ATCC 16045 and carbon and nitrogen balances

Food Technology and Biotechnology

Volumn 44, Issue 4, 2006, Pages 479-483

  Silva Santisteban, Bernardo Onagar Yépez ^a   Converti, Attilio ^b   Filho, Francisco Maugeri ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

^b UNIVERSITY OF GENOA   (Italy)

Author keywords

Carbon source; Inulinase production; Kluyveromyces marxianus; Nitrogen source

Indexed keywords

CARBON SOURCE; CULTIVATION CONDITIONS; FAST PROTEIN LIQUID CHROMATOGRAPHY; INTRINSIC ACTIVITIES; INULINASE; KLUYVEROMYCES MARXIANUS; NITROGEN SOURCES; SDS-PAGE ELECTROPHORESIS;

CARBON; CULTIVATION; ELECTROPHORESIS; LIQUID CHROMATOGRAPHY;

NITROGEN;

KLUYVEROMYCES; KLUYVEROMYCES MARXIANUS;

EID: 33845451681     PISSN: 13309862     EISSN: 13309862     Source Type: Journal    
DOI: None     Document Type: Article

References (24)

1. A. Converti, J.M. Domínguez, Influence of temperature and pH on xylitol production from xylose by Debaryomyces hansenii, Biotechnol Bioeng. 75 (2001) 39-45.
2. A. Converti, P. Perego, A. Sordi, P. Torre, Effect of starting xylose concentration on the microaerobic metabolism of Debaryomyces hansenii: The use of carbon material balances, Appl. Biochem. Biotechnol. 101 (2002) 15-29.
3. B. Rivas, P. Torre, J.M. Domínguez, P. Perego, A. Converti, J.C. Parajó, Carbon material and bioenergetic balances of xylitol production from corncobs by Debaryomyces hansenii, Biotechnol. Progr. 19 (2003) 706-713.
4. F.C. Sampaio, P. Torre, F.M.L. Passos, P. Perego, F.J.V. Passos, A. Converti, Xylose metabolism in Debaryomyces hansenii UFV-170. Effect of the specific oxygen uptake rate, Biotechnol. Progr. 20 (2004) 1641-1650.
5. W. Carvalho, J.C. Santos, L. Canilha, S.S. Silva, P. Perego, A. Converti, Xylitol production from sugarcane bagasse hydrolysate, Biochem. Eng. J. 25 (2005) 25-31.
6. J.C. Santos, A. Converti, W. Carvalho, S.I. Mussatto, S.S. Silva, Influence of aeration rate and carrier concentration on xylitol production from sugarcane bagasse hydrolyzate in immobilized-cell fluidized bed reactor, Process Biochem. 40 (2005) 113-118.
7. D. De Faveri, P. Torre, F. Molinari, P. Perego, A. Converti, Carbon material balances and bioenergetics of 2,3-butanediol bio-oxidation by Acetobacter hansenii, Enzyme Microb. Technol. 33 (2003) 708-719.
8. A. Converti, P. Perego, Use of carbon and energy balances in the study of the anaerobic metabolism of Enterobacter aerogenes at variable starting glucose concentrations, Appl. Microbiol Biotechnol. 59 (2002) 303-309.
9. A. Converti, P. Perego, M. Del Borghi, Effect of specific oxygen uptake rate on Enterobacter aerogenes energetics: Carbon and reduction degree balances in batch cultivations, Biotechnol. Bioeng. 82 (2003) 370-377.
10. J.W.D. GrootWassink, S.E. Fleming, Non-specific β-fructofuranosidase (inulinase) from Kluyveromyces fragilis: Batch and continuous fermentation, simple recovery method and some industrial properties, Enzyme Microb. Technol. 2 (1980) 45-53.
11. M. Manzoni, V. Cavazzoni, Extracellular inulinase from four yeasts, Lebensm. Wiss. Technol. - Food Sci. Technol. 21 (1988) 271-274.
12. R. Azhari, A.M. Szlak, E. Ilan, S. Sideman, N. Lotan, Purification and characterization of endo- and exo-inulinase, Biotechnol. Appl. Biochem. 11 (1989) 105-117.
13. A. Pandey, C.R. Soccol, P. Selvakumar, V.T. Soccol, N. Krieger, J. D. Fontana, Recent developments in microbial inulinases. Its production, properties, and industrial applications, Appl. Biochem. Biotechnol. 81 (1999) 35-52.
14. R.J. Rouwenhorst, M. Hensing, J. Verbakel, A.W. Scheffers, P. van Dijken, Structure and properties of the extracellular inulinase of Kluyveromyces marxianus CBS 6556, Appl. Environ. Microbiol. 56 (1990) 3337-3345.
15. O.H. Lowry, N.J. Rosenbrough, A.L. Farr, R.J. Randall, Protein measurement with the Folin phenol reagent, J. Biol. Chem. 193 (1951) 265-275.
16. M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72 (1976) 248-254.
17. R.J. Rouwenhorst, J.F. Jzn, W.A. Scheffers, J.P. van Dijken, Determination of protein concentration by total organic carbon analysis, J. Biochem. Biophys. Methods, 22 (1991) 119-128.
18. S.J. Kalil, R. Suzan, F. Maugeri, M.I. Rodrigues, Optimization of inulinase production by Kluyveromyces marxianus using factorial design, Appl. Biochem. Biotechnol. 94 (2001) 257-264.
19. C. Verduyn, E. Postma, W.A. Scheffers, J.P. van Dijken, Effect of benzoic acid on metabolic fluxes in yeasts: A continuous-culture study on the regulation of respiration and alcoholic fermentation, Yeast, 8 (1992) 501-517.
20. D. Vranesick, Z. Kurtanjek, A.M.P. Santos, F. Maugeri, Optimisation of inulinase production by Kluyveromyces bulgaricus, Food Technol. Biotechnol. 40 (2002) 67-73.
21. A. Santos: Synthesis of Oligosaccharides by Inulinase of Kluyveromyces bulgaricus, MSc Thesis, Faculty of Food Engineering, University of Campinas, Campinas SP, Brazil (1998).
22. G.L. Miller, Use of dinitrosalicylic acid reagent for determination of reducing sugar, Anal. Chem. 31 (1959) 426-428.
23. O. Laloux, J.P. Cassart, J. Delcour, J.V.E. Beeumen, J. Vandenhaute, Cloning and sequencing of the inulinase gene of Kluyveromyces marxianus var. marxianus ATCC 12424, FEBS Lett. 289 (1991) 64-68.
24. C.L. Flores, C. Rodriguez, T. Petit, C. Gancedo, Carbohydrate and energy-yielding metabolism in non-conventional yeasts, FEMS Microbiol. Rev. 24 (2005) 507-529.