Plasma holotranscobalamin compared with plasma cobalamins for assessment of vitamin B12 absorption; optimisation of a non-radioactive vitamin B12 absorption test (CobaSorb)

Clinica Chimica Acta

Volumn 376, Issue 1-2, 2007, Pages 150-154

  Hvas, Anne Mette ^a   Morkbak, Anne L ^a   Nexo, Ebba ^a  

^a AARHUS UNIVERSITY HOSPITAL   (Denmark)

Author keywords

Absorption test; Cobalamin; Holotranscobalamin; Vitamin B12

Indexed keywords

COBALAMIN; COBALAMIN DERIVATIVE; CYANOCOBALAMIN; HOLOTRANSCOBALAMIN;

ADULT; AGED; ARTICLE; BLOOD SAMPLING; COMPARATIVE STUDY; CONTROLLED STUDY; DIET RESTRICTION; FEMALE; HUMAN; HUMAN EXPERIMENT; MAJOR CLINICAL STUDY; MALE; NORMAL HUMAN; PERFORMANCE; PLASMA; PRIORITY JOURNAL; SCHILLING TEST; VITAMIN METABOLISM;

ADULT; AGED; AGED, 80 AND OVER; BIOLOGICAL MARKERS; BLOOD CHEMICAL ANALYSIS; FEMALE; HUMANS; INTESTINAL ABSORPTION; MALE; MIDDLE AGED; RADIOISOTOPES; TRANSCOBALAMINS; VITAMIN B 12;

EID: 33845337644     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cca.2006.08.009     Document Type: Article

References (18)

1. Moestrup S.K., and Verroust P.J. Megalin- and cubilin-mediated endocytosis of protein-bound vitamins, lipids, and hormones in polarized epithelia. Annu Rev Nutr 21 (2001) 407-428
2. Kristiansen M., Kozyraki R., Jacobsen C., Nexo E., Verroust P.J., and Moestrup S.K. Molecular dissection of the intrinsic factor-vitamin B12 receptor, cubilin, discloses regions important for membrane association and ligand binding. J Biol Chem 274 (1999) 20540-20544
3. He Q., Madsen M., Kilkenney A., et al. Amnionless function is required for cubilin brush-border expression and intrinsic factor-cobalamin (vitamin B12) absorption in vivo. Blood 106 (2005) 1447-1453
4. Chanarin I. The megaloblastic anaemias. Third ed. (1990), Blackwell Scientific Publications, London
5. Seetharam B., and Yammani R.R. Cobalamin transport proteins and their cell-surface receptors. Expert Rev Mol Med 5 (2003) 1-18
6. Schilling R.F. Intrinsic factor studies II. The effect of gastric juice on the urinary excretion of radioactivity after the oral administration of radioactive vitamin B12. J Lab Clin Med 42 (1953) 860-865
7. Bor M.V., Nexo E., and Hvas A.M. Holo-transcobalamin concentration and transcobalamin saturation reflect recent vitamin B12 absorption better than does serum vitamin B12. Clin Chem 50 (2004) 1043-1049
8. Bor M.V., Cetin M., Aytac S., Altay C., and Nexo E. Nonradioactive vitamin B12 absorption test evaluated in controls and in patients with inherited malabsorption of vitamin B12. Clin Chem 51 (2005) 2151-2155
9. Nexo E., Christensen A.L., Petersen T.E., and Fedosov S.N. Measurement of transcobalamin by ELISA. Clin Chem 46 (2000) 1643-1649
10. Nexo E., Christensen A.L., Hvas A.M., Petersen T.E., and Fedosov S.N. Quantitation of holo-transcobalamin, a marker of vitamin B12 deficiency. Clin Chem 48 (2002) 561-562
11. Morkbak A.L., Heimdal R.M., Emmens K., et al. Evaluation of the technical performance of novel holotranscobalamin (holoTC) assays in a multicenter European demonstration project. Clin Chem Lab Med 43 (2005) 1058-1064
12. Nexo E., Nooroya B., Hvas A.M., Christensen A.L., and Waters H. Autoantibodies against intrinsic factor (IF) measured with an ELISA using recombinant human IF as both catching and detecting reagent. Clin Chem Lab Med 43 (2005) 351-356
13. Rasmussen K. Solid-phase sample extraction for rapid determination of methylmalonic acid in serum and urine by a stable-isotope-dilution method. Clin Chem 35 (1989) 260-264
14. Moller J., Ahola L., and Abrahamsson L. Evaluation of the DPC IMMULITE 2000 assay for total homocysteine in plasma. Scand J Clin Lab Invest 62 (2002) 369-373
15. Nexø E. Variation with age of reference values for P-cobalamins. Scand J Haematol 30 (1983) 430-432
16. Rasmussen K., Moller J., Lyngbak M., Pedersen A.M., and Dybkjaer L. Age- and gender-specific reference intervals for total homocysteine and methylmalonic acid in plasma before and after vitamin supplementation. Clin Chem 42 (1996) 630-636
17. Nilsson-Ehle H., Jagenburg R., Landahl S., Lindstedt G., Swolin B., and Westin J. Cyanocobalamin absorption in the elderly: results for healthy subjects and for subjects with low serum cobalamin concentration. Clin Chem 32 (1986) 1368-1371
18. Hvas A.M., Buhl H., Laursen N.B., Hesse B., Berglund L., and Nexo E. The effect of recombinant human intrinsic factor on the uptake of vitamin B12 in patients with evident vitamin B12 deficiency. Haematologica 916 (2006) 805-808