Detection of Dientamoeba fragilis in fresh stool specimens using PCR

International Journal for Parasitology

Volumn 35, Issue 1, 2005, Pages 57-62

  Stark, D ^a   Beebe, N ^b   Marriott, D ^a   Ellis, J ^c   Harkness, J ^a  

^a ST VINCENT S HOSPITAL   (Australia)

^b UNIVERSITY OF TECHNOLOGY SYDNEY   (Australia)

^c UNIVERSITY OF TECHNOLOGY SYDNEY   (Australia)

Author keywords

Diagnosis; Dientamoeba fragilis; Faeces; PCR; Ribosomal DNA

Indexed keywords

DETECTION METHOD; FECES; PARASITIC DISEASE; POLYMERASE CHAIN REACTION;

ARTICLE; DIENTAMOEBA; DNA EXTRACTION; FECES; FECES ANALYSIS; GENE AMPLIFICATION; HUMAN; MICROORGANISM DETECTION; PARASITE IDENTIFICATION; PARASITOLOGY; POLYMERASE CHAIN REACTION; PROTOZOAL INFECTION; RNA GENE; SENSITIVITY AND SPECIFICITY; ANIMAL; DIARRHEA; DIFFERENTIAL DIAGNOSIS; EVALUATION; GENE; GENETICS; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR GENETICS; SARCOMASTIGOPHORA INFECTION;

DIENTAMOEBA; DIENTAMOEBA FRAGILIS; PROTOZOA;

PROTOZOAL DNA;

ANIMALS; DIAGNOSIS, DIFFERENTIAL; DIARRHEA; DIENTAMOEBA; DIENTAMOEBIASIS; DNA, PROTOZOAN; FECES; GENES, PROTOZOAN; GENES, RRNA; HUMANS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 20444503549     PISSN: 00207519     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ijpara.2004.09.003     Document Type: Article

References (27)

1. Blessmann, J., Buss, H., Ton Nu, P.A., Dinh, B.T., Viet Ngo, Q.T., Le Van, A., Abd Alla, M.D., Jackson, F.H.G., Ravdin, J.I., Tannich, E., 2002. Real-time PCR for detection and differentiation of Entamoeba histolytica and Entamoeba dispar in fecal samples. J. Clin. Microbiol. 40, 4413-4417.
2. Brug, S.L., 1936. Observations on D. fragilis. Ann. Trop. Med. Parasit. 30, 441.
3. Butler, W.P., 1996. Dientamoeba fragilis. An unusual intestinal pathogen. Dig. Dis. Sci. 41, 1811-1813.
4. Camp, R.R., Mattern, C.F.T., Honigberg, B.M., 1974. Study of Dientamoeba fragilis Jepps and Dobell. I. Electron microscopy observations of the binucleate stages. II. Taxonomic position and revision of the genus. J. Protozool. 21, 69-82.
5. Clark, C.G., Diamond, L.S., 2002. Methods for cultivation of luminal parasitic protists of clinical importance. Clin. Microbiol. Rev. 15 (3), 329-341.
6. Delgado-Viscogliosi, P., Viscogliosi, E., Gerbod, D., Kulda, J., Sogin, M.L., Edgcomb, V.P., 2000. Molecular phylogeny of Parabasalids based on small subunit rRNA sequences, with emphasis on the Trichomonadinae subfamily. J. Eukar. Microbiol. 47 (1), 70-75.
7. Dobell, C., 1940. Researches on the intestinal protozoa of monkeys and man. X. The life history of Dientamoeba fragilis: observations, experiments and speculations. Parasitology. 32, 411-461.
8. Dwyer, D.M., 1972. Analysis of the antigenic relationship among Trichomonas, Histomonas, Dientamoeba and Entamoeba. II. Gel diffusion methods. J. Protozool. 19, 326-332.
9. Garcia, L.S., 2001. Diagnostic Medical Parasitology. ASM Press, Washington, DC.
10. Girginkardesler, N., Coskun, S., Cuneyt Balcioglu, I., Ertan, P., Ok, U.Z., 2003. Dientamoeba fragilis, a neglected cause of diarrhoea, successfully treated with secnidazole. Clin. Microbiol. Infect. 9 (2), 110-113.
11. Hakansson, E.G., 1936. Dientamoeba fragilis, a cause of illness: report of a case. Am. J. Trop. Med. 16, 175-183.
12. Johnson, J.A., Clarke, G.C., 2000. Cryptic diversity in Dientamoeba fragilis. J. Clin. Microbiol. 12, 4653-4654.
13. Lantz, P.G., Matsson, M., Wadstrom, T., Radstrom, P., 1997. Removal of PCR inhibitors from human fecal samples through the use of an aqueous two-phase system for sample preparation prior to PCR. J. Clin. Microbiol. Methods 28, 159-167.
14. Limor, J.R., Lal, A.A., Xiao, L., 2002. Detection and differentiation of Cryptosporidium parasites that are pathogenic for humans by real-time PCR. J. Clin. Microbiol. 40, 2335-2338.
15. McOrist, A.L., Jackson, M., Bird, A.R., 2002. A comparison of five methods for extraction of bacterial DNA from human faecal samples. J. Microbiol. Method 50 (2), 131-139.
16. Peek, R., Reedeker, F.R., van Gool, T., 2004. Direct amplification and genotyping of Dientamoeba fragilis from human stool specimens. J. Clin. Microbiol. 42, 631-635.
17. Priess, U., Ockert, G., Broemme, S., Otto, A., 1991. On the clinical importance of Dientamoeba fragilis infections in children. J. Hyg. Epidemiol. Microbiol. Immunol. 35, 27-34.
18. Ramos, F., Zurabian, R., Moran, P., Ramiro, M., Gomez, A., Clark, G.C., Melendro, E.I., Garcia, G., Ximenez, C., 1999. The effect of formalin fixation on the polymerase chain reaction characterization of Entamoeba histolytica. Trans. R. Soc. Trop. Med. Hyg. 93, 335-336.
19. Sawangjaroen, N., Luke, R., Prociv, P., 1993. Diagnosis by faecal culture of Dientamoeba fragilis infections in Australian patients with diarrhoea. Trans R Soc Trop Med Hyg. 87, 163-165.
20. Silberman, J.D., Clark, G.C., Sogin, M.L., 1996. Dientamoeba fragilis shares a recent common evolutionary history with the trichomonads. Mol. Biochem. Parasitol. 76, 311-314.
21. Talis, B., 1967. Antigenic relationships among strains of Entamoeba histolytica, Dientamoeba fragilis and Entamoeba invadens. J. Protozool. 14, 44.
22. Troll, H., Marti, H., Weiss, N., 1997. Simple differential detection of Entamoeba histolytica and Entamoeba dispar in fresh stool specimens by sodium acetate-acetic-acid-formalin concentration and PCR. J. Clin. Microbiol. 35, 1701-1705.
23. van Gool, T., Weijts, R., Lommerse, E., Mank, T.G., 2003. The triple faeces test: an effective tool for the detection of intestinal parasites in routine clinical practice. Eur. J. Clin. Microbiol. Infect., Dis. 22, 284-290.
24. Wenrich, D.H., 1944. Studies on Dientamoeba fragilis (protozoa): IV. Further observations, with an outline of present-day knowledge of this species. J. Parasitol. 30, 322-327.
25. Windsor, J.J., Johnson, E.H., 1999. Dientamoeba fragilis: the unflagellated human flagellate. Br. J. Biomed. Sci. 56, 293-306.
26. Windsor, J.J., Macfarlane, L., Hughes-Thapa, G., Jones, S.K., Whiteside, T.M., 2003. Detection of Dientamoeba fragilis by culture. Br. J. Biomed. Sci. 60 (2), 79-83.
27. Yang, J., Scholten, T.H., 1977. Dientamoeba fragilis: a review with notes on its epidemiology, pathogenicity, mode of transmission and diagnosis. Am. J. Trop. Med. Hyg. 26, 16-22.