Viability and acrosome staining of stallion spermatozoa by Chicago sky blue and Giemsa

Biotechnic and Histochemistry

Volumn 81, Issue 4-6, 2006, Pages 109-117

  Kutvolgyi G ^a   Stefler, J ^a   Kovacs A ^a  

^a SZENT ISTVÁN UNIVERSITY   (Hungary)

Author keywords

Acrosome integrity; Densitometry; Light microscopy; Membrane integrity; Spermatozoa; Stallion; Viability

Indexed keywords

NEUTRAL RED; TRYPAN BLUE;

ACROSOME; ANIMAL CELL; ARTICLE; CELL DAMAGE; CELL DIFFERENTIATION; CELL STRUCTURE; CELL TYPE; CELL VIABILITY; CHEMICAL STRUCTURE; CONTROLLED STUDY; CYTOCHEMISTRY; DENSITOMETRY; DIGITAL IMAGING; FREEZE THAWING; GIEMSA STAIN; HISTOGRAM; IMAGE ANALYSIS; INTERMETHOD COMPARISON; MALE; NONHUMAN; RELIABILITY; REPRODUCIBILITY; SEMEN ANALYSIS; SPERMATOZOON; SPERMATOZOON MOTILITY; SPERMATOZOON TAIL; STALLION;

ACROSOME; ANIMALS; AZURE STAINS; CELL SURVIVAL; COLORING AGENTS; DENSITOMETRY; HORSES; MALE; SPERMATOZOA; STAINING AND LABELING; TRYPAN BLUE;

EQUIDAE;

EID: 33751564841     PISSN: 10520295     EISSN: 14737760     Source Type: Journal    
DOI: 10.1080/10520290600931007     Document Type: Article

References (21)

1. Assumpcao, MEOA and Buratini, J, Jr and Haipeck, K and Lima, AS and Mello, MRB and Oliveira, LJ and Oliveira, VP and Tavares, LMT and Visintin, JA. (2000) In vitro capacitation of bovine sperm by heparin and calcium ionophore evaluated with different staining methods Theriogenology, 53, pp. 485.
2. Bland, JM and Altman, DG. (1986) Statistical methods for assessing agreement between two methods of clinical measurement Lancet i, 8476, pp. 307-310.
3. British Standards Institution (1975) Precision of test methods. 1. Guide for the determination and reproducibility for a standard test method (BS 597, Part 1). London, BSI.
4. Cheng, TC and Huang, CC and Huang, LS and Chen, CI and Lee, MS and Liu, JY. (2004) Evaluation of mouse blastocyst implantation rate by morphology grading Chin. J. Physiol., 47, pp. 43-47.
5. Domes, U and Stolla, R. (2001) Staining of stallion spermatozoa with damaged tail membrane after freezing/thawing Anim. Reprod. Sci., 68, pp. 329-330.
6. Garside, DA and Charlton, A and Heath, KJ. (1996) Establishing the timing of implantation in the Harlan Porcellus Dutch and New Zealand white rabbit Reg. Tox. Pharm., 23, pp. 69-73.
7. Kovács, A and Foote, RH. (1992) Viability and acrosome staining of bull, boar and rabbit spermatozoa Biotech. & Histochem., 67, pp. 119-124.
8. Kovács A, Foote RH, Nagy Sz, Boersma A, Leidl W, Stolla R, Domes U (2000) Live/dead and acrosome staining of stallion spermatozoa. 14th International Congress on Animal Reproduction, Stockholm. 2-6July2000. p. 82.
9. Lillie RD (1977) H. J. Conn's Biological Stains, 9th ed., Williams & Wilkins Co., Baltimore. pp. 158-163.
10. Lundkvist, O and Ljungkvist, I. (1977) Morphology of the rat endometrial stroma at the appearance of the pontamine blue reaction during implantation after an experimental delay Cell Tissue Res., 184, pp. 453-466.
11. Molnár, A and Sarlós, P and Fáncsi, G and Rátky, J and Nagy, Sz and Kovács, A. (2001) A sperm tail defect associated with infertility in a goat-a case report Acta Vet. Hung., 49, pp. 341-348.
12. Nagy, Sz and Házas, G and Bali Papp, Á and Iváncsics, J and Szász, F and Szász, F, Jr and Kovács, A and Foote, RH. (1999) Evaluation of sperm tail membrane integrity by light microscopy Theriogenology, 52, pp. 1153-1159.
13. Nagy Sz, Qi X, Han J, Kovács A (2000) Light microscopic investigations on frozen-thawed yak semen-a pilot study. Third Int. Congr. Yak (ICY), Lhasa, P.R. China.
14. Nagy, Sz and Kovács, A and Zubor, T and Zomborszky, Z and Toth, J and Horn, P. (2001) Evaluation of frozen/thawed deer spermatozoa: Short communication Acta Vet. Hung., 49, pp. 223-227.
15. Nagy Sz, Jansen J, Topper EK (2003a) Validation of a light microscopic analysis of bull sperm quality by flow cytometry. Reprod. Dom. Anim. 38. No 4. p 334. Abstract P3.
16. Nagy, Sz and Jansen, J and Topper, EK and Gadella, BM. (2003b) A triple-stain flow cytometric method to assess plasma- and acrosome-membrane integrity of cryopreserved bovine sperm immediately after thawing in the presence of egg-yolk particles Biol. Reprod., 68, pp. 1828-1835.
17. Petrie A, Watson P (1999) Statistics for Veterinary and Animal Science. Blackwell Science Ltd., London. p. 243.
18. Presicce GA, Révay T, Nagy Sz, Dinnyés A, Kovács A (2003) Complex staining of water buffalo (Bubalus bubalis) spermatozoa. Bubalus bubalis II/2003: 55-60.
19. Sarhaddi, F and Iváncsics, J and Gergátz, E. (1995) Effect of handling on viability and acrosome status of ram spermatozoa Acta Agron. Ovárien., 37, pp. 193-198.
20. Somfai, T and Bodó, Sz and Nagy, Sz and Gócza, E and Iváncsics, J and Kovács, A. (2002) Simultaneous evaluation of viability and acrosome integrity of mouse spermatozoa using light microscopy Biotech. & Histochem., 77, pp. 117-120.
21. Vidament, M and Ecot, P and Noue, P and Bourgeois, C and Magistrini, M and Palmer, E. (2000) Centrifugation and addition of glycerol at 22° C instead of 4° C improve post-thaw motility and fertility of stallion spermatozoa Theriogenology, 54, pp. 907-919.