Simultaneous evaluation of viability and acrosome integrity of mouse spermatozoa using light microscopy

Biotechnic and Histochemistry

Volumn 77, Issue 3, 2002, Pages 117-120

  Somfai, T ^a   Bodo Sz ^a   Gocza E ^a   Ivancsics J ^a   Kovács, András ^a  

^a SZENT ISTVÁN UNIVERSITY   (Hungary)

Author keywords

Acrosome integrity; Light microscopy; Mouse; Spermatozoa; Viability

Indexed keywords

CONGO RED; FIXATIVE; NEUTRAL RED; TRYPAN BLUE;

ACROSOME; ANIMAL CELL; ARTICLE; CELL MEMBRANE; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; GIEMSA STAIN; MICROSCOPY; MOUSE; NONHUMAN; RABBIT; SPERMATOZOON; SPERMATOZOON ABNORMALITY; STAINING; ANIMAL; C57BL MOUSE; CELL SURVIVAL; CYTOLOGY; MALE; PHYSIOLOGY;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

ACROSOME; ANIMALS; CELL SURVIVAL; MALE; MICE; MICE, INBRED C57BL; SPERMATOZOA; STAINING AND LABELING; TRYPAN BLUE;

EID: 0036020718     PISSN: 10520295     EISSN: None     Source Type: Journal    
DOI: 10.1080/bih.77.3.117.120     Document Type: Article

References (9)

1. Methods for evaluating the acrosomal status of mammalian sperm
2. Detection and kinetics of the normal acrosome reaction of mouse sperm
3. Viability and acrosome staining of bull, boar and rabbit spermatozoa
4. Simple histochemical stain for acrosomes on sperm from several species
5. The role of carbohydrates in the induction of the acrosome reaction in mouse spermatozoa
6. Evaluation of sperm tail membrane integrity by light microscopy
7. Capacitation of bovine sperm by heparin
8. Evaluation of mouse sperm acrosomal status and viability by flow cytometry
9. Maturation of mammalian spermatozoa: Modifications of the acrosome and plasma membrane leading to fertilization