The grapevine polygalacturonase-inhibiting protein (VvPGIP1) reduces Botrytis cinerea susceptibility in transgenic tobacco and differentially inhibits fungal polygalacturonases

Transgenic Research

Volumn 15, Issue 6, 2006, Pages 687-702

  Joubert, Dirk A ^a   Slaughter, Ana R ^b   Kemp, Gabré ^c   Becker, John V W ^a   Krooshof, Geja H ^e   Bergmann, Carl ^d   Benen, Jacques ^e   Pretorius, Isak S ^f   Vivier, Melané A ^a  

^a STELLENBOSCH UNIVERSITY   (South Africa)

^b UNIVERSITY OF NEUCHÂTEL   (Switzerland)

^c UNIVERSITY OF THE FREE STATE   (South Africa)

^d UNIVERSITY OF GEORGIA   (United States)

^e WAGENINGEN UNIVERSITY   (Netherlands)

^f AUSTRALIAN WINE RESEARCH INSTITUTE   (Australia)

Author keywords

Aspergillus niger; Botrytis cinerea; Polygalacturonase inhibiting protein; Polygalacturonases; Vitis vinifera

Indexed keywords

PGIP PROTEIN, PLANT; POLYGALACTURONASE; VEGETABLE PROTEIN;

ARTICLE; BOTRYTIS; CHEMISTRY; DISEASE PREDISPOSITION; DRUG ANTAGONISM; DRUG EFFECT; ENZYMOLOGY; GENETICS; GRAPE; IMMUNOLOGY; MICROBIOLOGY; PATHOGENICITY; TOBACCO; TRANSGENIC PLANT;

BOTRYTIS; DISEASE SUSCEPTIBILITY; PLANT PROTEINS; PLANTS, GENETICALLY MODIFIED; POLYGALACTURONASE; TOBACCO; VITIS;

ASPERGILLUS; ASPERGILLUS NIGER; BOTRYOTINIA FUCKELIANA; BOTRYTIS; FUNGI; NICOTIANA TABACUM; VITIS; VITIS SP.; VITIS VINIFERA;

EID: 33751501231     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-006-9019-1     Document Type: Article

References (36)

1. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
2. Carstens M, Vivier MA, Pretorius IS (2003) The Saccharomyces cerevisiae chitinase, encoded by the CTS1-2 gene, confers antifungal activity against Botrytis cinerea to transgenic tobacco. Transgenic Res 12:497-508
3. Davies C, Robinson SP (2000) Differential screening indicates a dramatic change in mRNA profiles during grape berry ripening. Cloning and characterization of cDNAs encoding putative cell wall and stress response proteins. Plant Physiol 122:803-812
4. De Ascensao A (2001) Isolation and characterization of a polygalacturonase-inhibiting protein (PGIP) and its encoding gene from Vitis vinifera L. Ph.D. Thesis, Stellenbosch University, Stellenbosch, RSA
5. De Lorenzo G, Ferrari S (2002) Polygalacturonase-inhibiting proteins in defence against phytopathogenic fungi. Curr Opin Plant Biol 5:295-299
6. De Lorenzo G, Cervone F, Bellincampi D, Caprari C, Clark AJ, Desiderio A, Devoto A, Forrest R, Leckie F, Nuss L (1994) Polygalacturonase, PGIP and oligogalacturonides in cell-cell communication. Biochem Soc Trans 22:394-397
7. De Lorenzo G, D'Ovidio R, Cervone F (2001) The role of polygalacturonase-inhibiting proteins (PGIPs) in defence against pathogenic fungi. Annu Rev Phytopathol 39:313-335
8. Esquerré-Tugayé MT, Boudart G, Dumas B (1999) Cell wall-degrading enzymes, inhibitory proteins and oligosaccharides participate in the molecular dialogue between plants and pathogens. Plant Physiol Biochem 38:157-163
9. Favaron F, D'Ovidio RD, Porceddu E, Alghisi P (1994) Purification and molecular characterization of a soybean polygalacturonase-inhibiting protein. Planta 195:80-87
10. Federici L, Caprari C, Mattei B, Savino C, Di Matteo A, De Lorenzo G, Cervone F, Tsernoglou D (2001) Structural requirements of endopolygalacturonase for the interaction with PGIP (polygalacturonase-inhibiting protein). Proc Natl Acad Sci USA 98:13425
11. Ferrari S, Vairo D, Ausubel FM, Cervone F, De Lorenzo G (2003) Tandemly duplicated Arabidopsis genes that encode polygalacturonase-inhibiting proteins are regulated coordinately by different signal transduction pathways in response to fungal infection. Plant Cell 15:93-106
12. Gallois P, Marinho P (1995) Leaf disc transformation using Agrobacterium tumefaciens-expression of heterologous genes in tobacco. Methods Mol Biol 49:39-48
13. Gomathi V, Gnanamanickam SS (2004) Polygalacturonase-inhibiting proteins in plant defence. Curr Sci 87:1211-1217
14. Hahlbrock K, Scheel D, Logemann E, Nurnberger T, Parniske M, Reinold S, Sacks WR, Schmelzer E (1995) Oligopeptide elicitor-mediated defence gene activation in cultured parsley cells. Proc Natl Acad Sci USA 92:4150-4157
15. Herlache TC, Hotchkiss AT, Burr TJ, Collmer A (1997) Characterization of the Agrobacterium vitis pehA gene and comparison of the encoded polygalacturonase with the homologous enzymes from Erwinia carotovora and Ralstonia solanacearum. Appl Environ Microbiol 63:338
16. Hood EA, Gelvin SB, Melchers LS, Hoekema A (1993) New Agrobacterium helper plasmids for gene transfer to plants. Transgenic Res 2:208-218
17. Idnurm A, Howlett BJ (2001) Pathogenicity genes of phytopathogenic fungi. Mol Plant Pathol 2:241-255
18. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a selective and versatile gene fusion marker in higher plants. EMBO J 13:3901-3907
19. Kars I, Krooshof GH, Wagemakers L, Joosten R, Benen JAE, Van Kan JAL (2005) Necrotizing activity of five Botrytis cinerea endopolygalacturonases produced in Pichia pastoris. Plant J 43:213-225
20. Kemp G, Stanton L, Bergmann CW, Clay RP, Albersheim P, Darvill A (2004) Polygalacturonase-inhibiting proteins can function as activators of polygalacturonase. Mol Plant Microbe Interact 17:888-894
21. King D, Bergmann C, Orlando R, Benen JAE, Kester HCM, Visser J (2002) Use of amide exchange mass spectrometry to study conformational changes within the endopolygalacturonase II-homogalacturonan-polygalacturonase inhibiting protein system. Biochemistry 41:10225-10233
22. Krooshof GH, Joosten R, Kester HCM, Kars I, Van Kan JAL, Benen JAE (2004) Endopolygalacturonases from Botrytis cinerea: biochemical properties and interaction with inhibiting proteins. XIII Botrytis symposium. Antalya, Turkey
23. McGarvey P, Kaper JM (1991) A simple and rapid method for screening transgenic plants. Biotechniques 11:428-432
24. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473-497
25. Pařenicová L, Benen JAE, Kester HCM, Visser J (1998) PgaE encodes a fourth member of the endoploygalacturonases of Aspergillus niger. Eur J Biochem 251:72-80
26. Powell ALT, Van Kan JAL, ten Have A, Visser J, Greve LC, Bennett AB, Labavitch JM (2000) Transgenic expression of pear PGIP in tomato limits fungal colonisation. Mol Plant Microbe Interact 9:942-950
27. Reymond P, Grunberger S, Paul K, Muller M, Farmer EE (1995) Oligogalacturonide defence signals in plants: large fragments interact with the plasma membrane in vitro. Proc Natl Acad Sci USA 92:4145-4149
28. Salzman RA, Tikhonova I, Bordelon BP, Hasegawa PM, Bressan RA (1998) Coordinate accumulation of antifungal proteins and hexoses constitutes a developmentally controlled defence response during fruit ripening in grape. Plant Physiol 117:465-472
29. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY
30. Shieh MT, Brown RL, Whitehead MP, Cary JW, Cotty PJ, Cleveland TE, Dean RA (1997) Molecular genetic evidence for the involvement of a specific polygalacturonase, P2c, in the invasion and spread of Aspergillus flavus in cotton bolls. Appl Environ Microbiol 63:3548-3552
31. Taylor RJ, Secor GA (1988) An improved diffusion assay for quantifying the polygalacturonase content of Erwinia culture filtrates. Phytopathology 78:1101-1103
32. ten Have A, Mulder W, Visser J, Van Kan JAL (1998) The endopolygalacturonase gene Bcpg1 is required for full virulence of Botrytis cinerea. Mol Plant Microbe Interact 11:1009-1016
33. ten Have A, Oude Brueil W, Wubben J, Visser J, van Kan JAL (2001) Botrytis cinerea endopolygalacturonase genes are differentially expressed in various plant tissues. Fungal Genet Biol 33:97-105
34. ten Have A, Tenberge KB, Benen JAE, Tudzynski P, Visser J, van Kan JAL (2002) The contribution of the cell wall degrading enzymes to pathogenesis of fungal plant pathogens. In: Kempken F (ed) The Mycota XI, agricultural applications. Springer-Verlag, Berlin, pp 341-358
35. Wubben JP, Mulder W, ten Have A, van Kan JAL, Visser J (1999) Cloning and partial characterisation of endopolygalacturonase genes from Botrytis cinerea. Appl Environ Microbiol 65:1596-1602
36. York WS, Darvill AG, McNiel M, Stevenson TT, Albersheim P (1985) Isolation and characterization of plant cell walls and plant cell wall components. Methods Enzymol 118:3-40