Influence of thrombin concentration on the mechanical and morphological properties of cell-seeded fibrin hydrogels

Acta Biomaterialia

Volumn 3, Issue 1, 2007, Pages 59-67

  Rowe, Shaneen L ^a   Lee, SungYun ^a   Stegemann, Jan P ^a  

^a RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

Fibrin; Smooth muscle cells; Thrombin; Tissue engineering

Indexed keywords

BIOMECHANICS; CELL ENGINEERING; CELL PROLIFERATION; COMPACTION; GELS; HYDROGELS; MUSCLE; SCANNING ELECTRON MICROSCOPY; TISSUE ENGINEERING;

CATALYSE; CELL DELIVERY; FIBRIN; GEL COMPACTION; MECHANICAL; MICROFIBRIL; MORPHOLOGICAL PROPERTIES; SMOOTH MUSCLE CELLS; TISSUE ENGINEERING APPLICATIONS; TISSUES ENGINEERINGS;

ENZYMES;

ACTIN; COLLAGEN TYPE 1; FIBRIN; THROMBIN;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL COUNT; CELL CULTURE; CELL FUNCTION; CELL PROLIFERATION; CONCENTRATION RESPONSE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; CYTOSKELETON; HYDROGEL; MORPHOLOGY; MUSCLE CELL; NONHUMAN; PARTICLE SIZE; POLYMERIZATION; PRIORITY JOURNAL; RAT; SCANNING ELECTRON MICROSCOPY; STRUCTURE ACTIVITY RELATION; TENSILE STRENGTH; THREE DIMENSIONAL IMAGING; TISSUE ENGINEERING; VASCULAR SMOOTH MUSCLE;

EID: 33751324100     PISSN: 17427061     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.actbio.2006.08.006     Document Type: Article

References (28)

1. Trentin D., Hall H., Wechsler S., and Hubbell J.A. Peptide-matrix-mediated gene transfer of an oxygen-insensitive hypoxia-inducible factor-1{alpha} variant for local induction of angiogenesis. Proc Natl Acad Sci USA 103 8 (2006) 2506-2511
2. Schmoekel H.G., Weber F.E., Schense J.C., Gratz K.W., Schawalder P., and Hubbell J.A. Bone repair with a form of BMP-2 engineered for incorporation into fibrin cell ingrowth matrices. Biotechnol Bioeng 89 3 (2005) 253-262
3. Grassl E.D., Oegema T.R., and Tranquillo R.T. Fibrin as an alternative biopolymer to type-I collagen for the fabrication of a media equivalent. J Biomed Mater Res 60 (2002) 607-612
4. Cummings C.L., Gawlitta D., Nerem R.M., and Stegemann J.P. Properties of engineered vascular constructs made from collagen, fibrin and collagen-fibrin mixtures. Biomaterials 17 (2004) 3699-3706
5. Swartz D.D., Russell J.A., and Andreadis S.T. Engineering of fibrin-based functional and implantable small-diameter blood vessels. Am J Physiol Heart Circ Physiol 288 (2005) H1451-H1460
6. Grassl E.D., Oegema T.R., and Tranquillo R.T. A fibrin-based arterial media equivalent. J Biomed Mater Res 66A (2003) 550-561
7. Yao L., Swartz D.D., Gugino S.F., Russel J.A., and Andreadis S.T. Fibrin-based tissue-engineering blood vessels: differential effects of biomaterial and culture parameters on mechanical strength and vasoreactivity. Tissue Eng 11 (2005) 991-1003
8. Rowe SL, Stegemann JP. Interpenetrating collagen-fibrin composite matrices with varying protein ratios and contents. Biomacromolecules 2006; 7(11), in press.
9. Neidert M.R., Lee E.S., Oegema T.R., and Tranquillo R.T. Enhanced fibrin remodeling with TGF-β1, insulin and plasmin for improved tissue equivalents. Biomaterials 23 (2002) 3717-3731
10. Ross J.J., and Tranquillo R.T. ECM gene expression correlates with in vitro tissue growth and development in fibrin gel remodeled by neonatal smooth muscle cells. Matrix Biol (2003) 477-490
11. Ferry J.D., and Morrison R.R. Preparation and properties of serum and plasma proteins. VIII. The conversion of human fibrinogen to fibrin under various conditions. J Am Chem Soc 69 (1947) 388-400
12. Wesley W.W., Kramer O., Rosser R.W., Nestler F.H.M., and Ferry J.D. Rheology of fibrin clots. I. Dynamic viscoelastic properties and fluid permeation. Biophys Chem 1 (1974) 152-160
13. Glover C.J., McIntire L.V., Brown III C.H., and Natelson E.A. Rheological properties of fibrin clots. Effects of fibrinogen concentration, Factor XIII deficiency, and Factor XIII inhibition. J Lab Clin Med 86 (1975) 644-656
14. Dhall T.Z., Bryce W.A., and Dhall D.P. Effects of dextran on the molecular structure and tensile behaviour of human fibrin. Thromb Haemost 35 (1976) 737-745
15. Weisel J.W., and Nagaswami C. Computer modeling of fibrin polymerization correlated with electron microscope and turbidity observations: clot structure and assembly are kinetically controlled. Biophys J 63 (1992) 111-128
16. Blombäck B., and Okada M. Fibrin gel structure and clotting time. Thromb Res 25 (1982) 51-70
17. Blombäck B., and Bark N. Fibrinopeptides and fibrin gel structure. Biophys Chem 112 (2004) 147-151
18. Blombäck B., Carlsson K., Hessel B., Liljeborg A., Procyk R., and Ǻslund N. Native fibrin gel networks observed by 3D microscopy, permeation and turbidity. Biochim Biophys Acta 997 (1989) 96-110
19. Kubota K., Kogure H., Masuda Y., Toyama Y., Kita R., Takahashi A., et al. Gelation dynamics and gel structure of fibrinogen. Colloids Surf B: Biointerfaces 38 (2004) 104-109
20. Herbert C.B., Nagaswami C., Bittner G.D., Hubbell J.A., and Weisel J.W. Effects of fibrin micromorphology on neurite growth from dorsal root ganglia cultured in three-dimensional fibrin gels. J Biomed Mater Res 40 (1998) 551-559
21. McNamara C.A., Sarembock I.J., Gimple L.W., Fenton II J.W., Coughlin S.R., and Owens G.K. Thrombin stimulates proliferation of cultured rat aortic smooth muscle cells by a proteolytically activated receptor. J Clin Invest 91 (1993) 94-98
22. Schauwienold D., Plum C., Helbing T., Voigt P., Bobbert T., Hoffmann D., et al. ERK1/2-dependent contractile protein expression in vascular smooth muscle cells. Hypertension 41 (2003) 546-552
23. Cox S., Cole M., and Tawil B. Behavior of human dermal fibroblasts in three-dimensional fibrin clots: dependence on fibrinogen and thrombin concentration. Tissue Eng 10 5/6 (2004) 942-954
24. Krishnan L.K., Lal A.V., Uma Shankar P.R., and Mohanty M. Fibrinolysis inhibitors adversely affect remodeling of tissues sealed with fibrin glue. Biomaterials 24 (2003) 321-327
25. Dikovsky D., Bianco-Peled H., and Seliktar D. The effect of structural alterations of PEG-fibrinogen hydrogel scaffolds on 3-D cellular morphology and cell migration. Biomaterials 19 (2005) 1496-1506
26. Pizzo A.M., Kokini K., Vaughn L.C., Waisner B.Z., and Voytik-Harbin S.L. Extracellular (ECM) microstructural composition regulates local cell-ECM biomechanics and fundamental fibroblast behavior: a multidimensional perspective. J Appl Physiol 98 (2005) 1909-1921
27. Karp J.M., Sarraf F., Shoichet M.S., and Davies J.E. Fibrin-filled scaffolds for bone-tissue engineering: an in vivo study. J Biomed Mater Res 71A (2004) 162-171
28. Barocas V.H., Girton T.S., and Tranquillo R.T. Engineered alignment in media equivalents: magnetic prealignment and mandrel compaction. J Biomech Eng 120 (1998) 660-666