Combination analysis of a whole lymph node by one-step nucleic acid amplification and histology for intraoperative detection of micrometastasis

Pathobiology

Volumn 73, Issue 4, 2006, Pages 183-191

  Taniyama, Kiyomi ^a   Motoshita, Junichi ^a   Sakane, Junichi ^a   Makita, Kyoko ^a   Akai, Yasumasa ^b   Daito, Motonari ^b   Otomo, Yasuhiro ^b   Ono, Hiroshi ^a   Mizunoe, Tomoya ^a   Takeuchi, Yukiyasu ^a   Tominaga, Harumi ^a   Koseki, Masato ^a  

^a NATIONAL HOSPITAL ORGANIZATION   (Japan)

^b SYSMEX CORPORATION   (Japan)

Author keywords

Histology; Lymph node metastasis; mRNA expression; Reverse transcription polymerase chain reaction

Indexed keywords

CYTOKERATIN 19; MESSENGER RNA;

ADULT; AGED; ANALYTIC METHOD; ARTICLE; CANCER CELL CULTURE; CELL COUNT; COLORECTAL CARCINOMA; CONTROLLED STUDY; FEMALE; GENE NUMBER; HISTOLOGY; HUMAN; HUMAN CELL; HUMAN TISSUE; LYMPH NODE; LYMPH NODE METASTASIS; MAJOR CLINICAL STUDY; MALE; METASTASIS; MICROMETASTASIS; NUCLEIC ACID AMPLIFICATION; ONE STEP NUCLEIC ACID AMPLIFICATION; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TECHNIQUE;

ADENOCARCINOMA; ADULT; AGED; AGED, 80 AND OVER; COLON; COLORECTAL NEOPLASMS; FEMALE; FROZEN SECTIONS; HUMANS; INTRAOPERATIVE PERIOD; KERATIN-19; LYMPH NODE EXCISION; LYMPH NODES; LYMPHATIC METASTASIS; MALE; MIDDLE AGED; NUCLEIC ACID AMPLIFICATION TECHNIQUES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA, NEOPLASM; TUMOR MARKERS, BIOLOGICAL;

EID: 33751322986     PISSN: 10152008     EISSN: None     Source Type: Journal    
DOI: 10.1159/000096019     Document Type: Article

References (16)

1. Taniyama K, Sasaki N, Maruyama H, et al: Carcinoma cells at deeply infiltrated sites: role in development of metastases in patients with advanced colorectal adenocarcinomas; in Tahara E (ed): Molecular Pathology of Gastroenterological Cancer: Application to Clinical Practice. Tokyo, Springer, 1997, pp 107-127.
2. Levin B, Raijman I: Malignant tumors of the colon and rectum; in Haubrich WS, Schaffner F, Berk JE (eds): Gastroenterology, ed 5. Philadelphia, Saunders, 1995, vol 2, pp 1744-1772.
3. Futamura M, Takagi Y, Koumura H, et al: Spread of colorectal cancer micrometastases in regional lymph nodes by reverse transcriptase-polymerase chain reactions for carcinoembryonic antigen and cytokeratin 20. J Surg Oncol 1998;68:34-40.
4. Liefers GJ, Cleton-Jansen AM, van de Velde CJ, et al: Micrometastases and survival in stage II colorectal cancer. N Engl J Med 1998;339:223-228.
5. Cutait R, Alves VA, Lopes LC, et al: Restaging of colorectal cancer based on the identification of lymph node micrometastases through immunoperoxidase staining of CEA and cytokeratins. Dis Colon Rectum 1991;34:917-920.
6. Miyake Y, Fujiwara Y, Ohue M, et al: Quantification of micrometastases in lymph nodes of colorectal cancer using real-time fluorescence polymerase chain reaction. Int J Oncol 2000;16:289-293.
7. Ho SB, Hyslop A, Albrecht R, et al: Quantification of colorectal cancer micrometastases in lymph nodes by nested and real-time reverse transcriptase-PCR analysis for carcinoembryonic antigen. Clin Cancer Res 2004;10:5777-5784.
8. Ikeda N, Miyoshi Y, Motomura K, Inaji H, Koyama H, Noguchi S: Prognostic significance of occult bone marrow micrometastases of breast cancer detected by quantitative polymerase chain reaction for cytokeratin 19 mRNA. Jpn J Cancer Res 2000;91:918-924.
9. Doglioni C, Dell'Orto P, Zanetti G, Iuzzolino P, Coggi G, Viale G: Cytokeratin-immunoreactive cells of human lymph nodes and spleen in normal and pathological conditions: an immunocytochemical study. Virchows Arch A Pathol Anat Histopathol 1990;416:479-490.
10. Notomi T, Okayama H, Masubuchi H, et al: Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 2000;28:E63.
11. Mori Y, Nagamine K, Tomita N, Notomi T: Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 2001;289:150-154.
12. Greene FL, Page DL, Fleming ID, et al (eds): AJCC Cancer Staging Handbook, ed 6. New York, Springer, 2002.
13. Japanese Research Society for Cancer of Colon and Rectum: General Rules for Clinical and Pathological Studies on Cancer of the Colon, Rectum and Anus, ed 6 (in Japanese). Tokyo, Kanehara, 1998.
14. Leong ASY, Cooper K, Leong FJWM: Manual of Diagnostic Antibodies for Immunohistology, ed 2. London, Greenwich Medical Media, 2003.
15. Matsuda J, Kitagawa Y, Fujii H, et al: Significance of metastasis detected by molecular techniques in sentinel nodes of patients with gastrointestinal cancer. Ann Surg Oncol 2004;11(suppl 3):250S-254S.
16. Zippelius A, Pantel K: RT-PCR-based detection of occult disseminated tumor cells in peripheral blood and bone marrow of patients with solid tumors: an overview. Ann NY Acad Sci 2000;906:110-123.