Co-expression of antibody fab heavy and light chain genes from separate evolved compatible replicons in E. coli

Journal of Immunological Methods

Volumn 317, Issue 1-2, 2006, Pages 56-63

  Leonard, Brandon ^a,b   Sharma, Vikram ^a   Smider, Vaughn ^a  

^a IntegriGen Inc   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Antibody engineering; ColE1; Compatible plasmids; Fab expression; Origin of replication

Indexed keywords

IMMUNOGLOBULIN F(AB) FRAGMENT;

ANTIBODY ENGINEERING; ANTIBODY LIBRARY; ARTICLE; ESCHERICHIA COLI; GENE; GENE EXPRESSION; HEAVY CHAIN; LIGHT CHAIN; NONHUMAN; PLASMID; PRIORITY JOURNAL; REPLICON;

ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; EVOLUTION; GENES, IMMUNOGLOBULIN HEAVY CHAIN; GENES, IMMUNOGLOBULIN LIGHT CHAIN; IMMUNOBLOTTING; IMMUNOGLOBULIN FAB FRAGMENTS; PLASMIDS; REPLICON; TRANSFORMATION, BACTERIAL;

EID: 33751310364     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2006.09.006     Document Type: Article

References (24)

1. Boder E.T., and Wittrup K.D. Yeast surface display for screening combinatorial polypeptide libraries. Nat. Biotechnol. 15 (1997) 553
2. Bolivar F., Rodriguez R.L., Greene P.J., Betlach M.C., Heyneker H.L., and Boyer H.W. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene 2 (1977) 95
3. Cane D.E., Walsh C.T., and Khosla C. Harnessing the biosynthetic code: combinations, permutations, and mutations. Science 282 (1998) 63
4. Collet T.A., Roben P., O'Kennedy R., Barbas III C.F., Burton D.R., and Lerner R.A. A binary plasmid system for shuffling combinatorial antibody libraries. Proc. Natl. Acad. Sci. U. S. A. 89 (1992) 10026
5. Daugherty P.S., Olsen M.J., Iverson B.L., and Georgiou G. Development of an optimized expression system for the screening of antibody libraries displayed on the Escherichia coli surface. Protein Eng. 12 (1999) 613
6. Hanes J., and Pluckthun A. In vitro selection and evolution of functional proteins by using ribosome display. Proc. Natl. Acad. Sci. U. S. A. 94 (1997) 4937
7. Hoogenboom H.R. Selecting and screening recombinant antibody libraries. Nat. Biotechnol. 23 (2005) 1105
8. Humphreys D.P., Carrington B., Bowering L.C., Ganesh R., Sehdev M., Smith B.J., King L.M., Reeks D.G., Lawson A., and Popplewell A.G. A plasmid system for optimization of Fab' production in Escherichia coli: importance of balance of heavy chain and light chain synthesis. Protein Expr. Purif. 26 (2002) 309
9. Jespers L.S., Roberts A., Mahler S.M., Winter G., and Hoogenboom H.R. Guiding the selection of human antibodies from phage display repertoires to a single epitope of an antigen. Biotechnology (N Y) 12 (1994) 899
10. Juarez-Gonzalez V.R., Riano-Umbarila L., Quintero-Hernandez V., Olamendi-Portugal T., Ortiz-Leon M., Ortiz E., Possani L.D., and Becerril B. Directed evolution, phage display and combination of evolved mutants: a strategy to recover the neutralization properties of the scFv version of BCF2 a neutralizing monoclonal antibody specific to scorpion toxin Cn2. J. Mol. Biol. 346 (2005) 1287
11. Kim D., Rhee Y., Rhodes D., Sharma V., Sorenson O., Greener A., and Smider V. Directed evolution and identification of control regions of ColE1 plasmid replication origins using only nucleotide deletions. J. Mol. Biol. 351 (2005) 763
12. Kohler G., and Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256 (1975) 495
13. Larrick J.W., Danielsson L., Brenner C.A., Abrahamson M., Fry K.E., and Borrebaeck C.A. Rapid cloning of rearranged immunoglobulin genes from human hybridoma cells using mixed primers and the polymerase chain reaction. Biochem. Biophys. Res. Commun. 160 (1989) 1250
14. Lewis S. The mechanism of V(D)J joining: lessons from molecular, immunological, and comparative analyses. Adv. Immunol. 56 (1994) 27
15. Novick R.P. Plasmid incompatibility. Microbiol. Rev. 51 (1987) 381
16. Ostermeier M., and Benkovic S.J. A two-phagemid system for the creation of non-phage displayed antibody libraries approaching one trillion members. J. Immunol. Methods 237 (2000) 175
17. Rajpal A., Beyaz N., Haber L., Cappuccilli G., Yee H., Bhatt R.R., Takeuchi T., Lerner R.A., and Crea R. A general method for greatly improving the affinity of antibodies by using combinatorial libraries. Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 8466
18. Reichert J.M., Rosensweig C.J., Faden L.B., and Dewitz M.C. Monoclonal antibody successes in the clinic. Nat. Biotechnol. 23 (2005) 1073
19. Smider V., and Chu G. The end-joining reaction in V(D)J recombination. Semin. Immunol. 9 (1997) 189
20. Smith G.P., and Petrenko V.A. Phage display. Chem. Rev. 97 (1997) 391
21. Studier F.W. Protein production by auto-induction in high density shaking cultures. Protein Expr. Purif. 41 (2005) 207
22. Ulrich H.D., Patten P.A., Yang P.L., Romesberg F.E., and Schultz P.G. Expression studies of catalytic antibodies. Proc. Natl. Acad. Sci. U. S. A. 92 (1995) 11907
23. Wilson D.S., Keefe A.D., and Szostak J.W. The use of mRNA display to select high-affinity protein-binding peptides. Proc. Natl. Acad. Sci. U. S. A. 98 (2001) 3750
24. Xie J., and Schultz P.G. Adding amino acids to the genetic repertoire. Curr. Opin. Chem. Biol. 9 (2005) 548