Interaction of human GTP cyclohydrolase I with its splice variants

Biochemical Journal

Volumn 400, Issue 1, 2006, Pages 75-80

  Pandya, Maya J ^a,b   Golderer, Georg ^a   Werner, Ernst R ^a   Werner Felmayer, Gabriele ^a  

^a INNSBRUCK MEDICAL UNIVERSITY   (Austria)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

Author keywords

Chinese hamster ovary cell (CHO cell); Co expression; Enzyme stability; GTP cyclohydrolase I; Splice variant; Tetrahydrobiopterin

Indexed keywords

AMINO ACIDS; AROMATIC COMPOUNDS; ENZYME IMMOBILIZATION; FILTRATION; GELS; OLIGOMERS;

CHINESE-HAMSTER OVARY CELL (CHO CELL); ENZYME STABILITY; SPLICE VARIANTS; TETRAHYDROBIOPTERIN;

ENZYMES;

GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE I; RECOMBINANT PROTEIN; TETRAHYDROBIOPTERIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME STABILITY; GEL CHROMATOGRAPHY; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; WESTERN BLOTTING;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; BLOTTING, WESTERN; CELL LINE; CHO CELLS; CHROMATOGRAPHY, GEL; CRICETINAE; CRICETULUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GTP CYCLOHYDROLASE; HUMANS; ISOENZYMES; MOLECULAR SEQUENCE DATA; PROTEIN BINDING; RECOMBINANT PROTEINS;

CRICETULUS GRISEUS; MAMMALIA;

EID: 33751085622     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20060765     Document Type: Article

References (31)

1. Thony, B., Auerbach, G. and Blau, N. (2000) Tetrahydrobiopterin biosynthesis, regeneration and functions. Biochem. J. 347, 1-16
2. Auerbach, G., Herrmann, A., Bracher, A., Bader, G., Gutlich, M., Fischer, M., Neukamm, M., Garrido-Franco, M., Richardson, J., Nar, H. et al. (2000) Zinc plays a key role in human and bacterial GTP cyclohydrolase I. Proc. Natl. Acad. Sci. U.S.A. 97, 13567-13572
3. Harada, T., Kagamiyama, H. and Hatakeyama, K. (1993) Feedback regulation mechanisms for the control of GTP cyclohydrolase I activity. Science 260, 1507-1510
4. Milstien, S., Jaffe, H., Kowlessur, D. and Bonner, T. I. (1996) Purification and cloning of the GTP cyclohydrolase I feedback regulatory protein, GFRP. J. Biol. Chem. 271, 19743-19751
5. Maita, N., Okada, K., Hatakeyama, K. and Hakoshima, T. (2002) Crystal structure of the stimulatory complex of GTP cyclohydrolase I and its feedback regulatory protein GFRP. Proc. Natl. Acad. Sci. U.S.A. 99, 1212-1217
6. Maita, N., Hatakeyama, K., Okada, K. and Hakoshima, T. (2004) Structural basis of biopterin-induced inhibition of GTP cyclohydrolase I by GFRP, its feedback regulatory protein. J. Biol. Chem. 279, 51534-51540
7. Hesslinger, C., Kremmer, E., Hultner, L., Ueffing, M. and Ziegler, I. (1998) Phosphorylation of GTP cyclohydrolase I and modulation of its activity in rodent mast cells. GTP cyclohydrolase I hyperphosphorylation is coupled to high affinity IgE receptor signaling and involves protein kinase C. J. Biol. Chem. 273, 21616-21622
8. Lapize, C., Pluss, C., Werner, E. R., Huwiler, A. and Pfeilschifter, J. (1998) Protein kinase C phosphorylates and activates GTP cyclohydrolase I in rat renal mesangial cells. Biochem. Biophys. Res. Commun. 251, 802-805
9. Nichol, C. A., Smith, G. K. and Duch, D. S. (1985) Biosynthesis and metabolism of tetrahydrobiopterin and molybdopterin. Annu. Rev. Biochem. 54, 729-764
10. Werner-Felmayer, G., Golderer, G. and Werner, E. R. (2002) Tetrahydrobiopterin biosynthesis, utilization and pharmacological effects. Curr. Drug Metab. 3, 159-173
11. Werner, E. R., Gorren, A. C., Heller, R., Werner-Felmayer, G. and Mayer, B. (2003) Tetrahydrobiopterin and nitric oxide: mechanistic and pharmacological aspects. Exp. Biol. Med. 228, 1291-1302
12. Thony, B. and Blau, N. (1997) Mutations in the GTP cyclohydrolase I and 6-pyruvoyltetrahydropterin synthase genes. Hum. Mutat. 10, 11-20
13. Hirano, M., Tamaru, Y., Nagai, Y., Ito, H., Imai, T. and Ueno, S. (1995) Exon skipping caused by a base substitution at a splice site in the GTP cyclohydrolase I gene in a Japanese family with hereditary progressive dystonia dopa responsive dystonia. Biochem. Biophys. Res. Commun. 213, 645-651
14. Hirano, M., Tamaru, Y., Ito, H., Matsumoto, S., Imai, T. and Ueno, S. (1996) Mutant GTP cyclohydrolase I mRNA levels contribute to dopa-responsive dystonia onset. Ann. Neurol. 40, 796-798
15. Hirano, M., Yanagihara, T. and Ueno, S. (1998) Dominant negative effect of GTP cyclohydrolase I mutations in dopa-responsive hereditary progressive dystonia. Ann. Neurol. 44, 365-371
16. Ichinose, H., Ohye, T., Takahashi, E., Seki, N., Hori, T., Segawa, M., Nomura, Y., Endo, K., Tanaka, H., Tsuji, S. and Nagatsu, T. (1994) Hereditary progressive dystonia with marked diurnal fluctuation caused by mutations in the GTP cyclohydrolase I gene. Nat. Genet. 8, 236-242
17. Suzuki, T., Ohye, T., Inagaki, H., Nagatsu, T. and Ichinose, H. (1999) Characterization of wild-type and mutants of recombinant human GTP cyclohydrolase I: relationship to etiology of dopa-responsive dystonia. J. Neurochem. 73, 2510-2516
18. Ichinose, H., Ohye, T., Matsuda, Y., Hori, T., Blau, N., Burlina, A., Rouse, B., Matalon, R., Fujita, K. and Nagatsu, T. (1995) Characterization of mouse and human GTP cyclohydrolase I genes. Mutations in patients with GTP cyclohydrolase I deficiency. J. Biol. Chem. 270, 10062-10071
19. Golderer, G., Werner, E. R., Heufler, C., Strohmaier, W., Grobner, P. and Werner-Felmayer, G. (2001) GTP cyclohydrolase I mRNA: novel splice variants in the slime mould Physarum polycephalum and in human monocytes (THP-1) indicate conservation of mRNA processing. Biochem. J. 355, 499-507
20. Togari, A., Ichinose, H., Matsumoto, S., Fujita, K. and Nagatsu, T. (1992) Multiple mRNA forms of human GTP cyclohydrolase I. Biochem. Biophys. Res. Commun. 187, 359-365
21. Hirano, M., Imaiso, Y. and Ueno, S. (1997) Differential splicing of the GTP cyclohydrolase I RNA in dopa-responsive dystonia. Biochem. Biophys. Res. Commun. 234, 316-319
22. Gutlich, M., Jaeger, E., Rucknagel, K. P., Werner, T., Rodl, W., Ziegler, I. and Bacher, A. (1994) Human GTP cyclohydrolase I: only one out of three cDNA isoforms gives rise to the active enzyme. Biochem. J. 302, 215-221
23. Hwu, W. L., Yeh, H. Y., Fang, S. W., Chiang, H. S., Chiou, Y. W. and Lee, Y. M. (2003) Regulation of GTP cyclohydrolase I by alternative splicing in mononuclear cells. Biochem. Biophys. Res. Commun. 306, 937-942
24. Schagger, H. and von Jagow, G. (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 KDa. Anal. Biochem. 166, 368-379
25. Werner, E. R., Wachter, H. and Werner-Felmayer, G. (1997) Determination of tetrahydrobiopterin biosynthetic activities by high-performance liquid chromatography with fluorescence detection. Methods Enzymol. 281, 53-61
26. Bradford, M. M. (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
27. Vie, A., Cigna, M., Toci, R. and Birman, S. (1999) Differential regulation of Drosophila tyrosine hydroxylase isoforms by dopamine binding and cAMP-dependent phosphorylation. J. Biol. Chem. 274, 16788-16795
28. Sura, G. R., Daubner, S. C. and Fitzpatrick, P. F. (2004) Effects of phosphorylation by protein kinase A on binding of catecholamines to the human tyrosine hydroxylase isoforms. J. Neurochem. 90, 970-978
29. McLean, J. R., Krishnakumar, S. and O'Donnell, J. M. (1993) Multiple mRNAs from the Punch locus of Drosophila melanogaster encode isoforms of GTP cyclohydrolase I with distinct N-terminal domains. J. Biol. Chem. 268, 27191-27197
30. Hwu, W. L., Lu, M. Y., Hwa, K. Y., Fan, S. W. and Lee, Y. M. (2004) Molecular chaperones affect GTP cyclohydrolase I mutations in dopa-responsive dystonia. Ann. Neurol. 55, 875-878
31. Roman, C., Cohn, L. and Calame, K. (1991) A dominant negative form of transcription activator mTFE3 created by differential splicing. Science 254, 94-97