Isolation and partial characterization of trehalose 6-phosphate synthase aggregates from Selaginella lepidophylla plants

Biochimie

Volumn 88, Issue 10, 2006, Pages 1505-1510

  Márquez Escalante, Jorge A ^a   Figueroa Soto, Ciria G ^a   Valenzuela Soto, Elisa M ^a  

^a CENTRO DE INVESTIGACIÓN EN ALIMENTACIÓN Y DESARROLLO   (Mexico)

Author keywords

Isoforms; Selaginella lepidophylla; Trehalose; Trehalose 6 phosphate synthase

Indexed keywords

ENZYME ANTIBODY; POLYPEPTIDE; POTASSIUM CHLORIDE; SYNTHETASE; TREHALOSE 6 PHOSPHATE SYNTHETASE; UNCLASSIFIED DRUG; GLUCOSYLTRANSFERASE; TREHALOSE-6-PHOSPHATE SYNTHASE;

AMINO ACID SEQUENCE; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; ELECTROPHORESIS; ELUTION; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME STABILITY; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; SELAGINELLA; SELAGINELLA LEPIDOPHYLLA; SEPARATION TECHNIQUE; TEMPERATURE; WESTERN BLOTTING; CHEMISTRY; CLASSIFICATION; ENZYMOLOGY; GEL CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; SELAGINELLACEAE;

SELAGINELLA LEPIDOPHYLLA;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; ENZYME STABILITY; GLUCOSYLTRANSFERASES; HYDROGEN-ION CONCENTRATION; MOLECULAR SEQUENCE DATA; SELAGINELLACEAE; TEMPERATURE;

EID: 33751068719     PISSN: 03009084     EISSN: 61831638     Source Type: Journal    
DOI: 10.1016/j.biochi.2006.06.004     Document Type: Article

References (38)

1. Gadd G.M., Chalmers K., and Reed R.H. The role of trehalose in dehydration resistance of Saccharomyces cerevisiae. FEMS Microbiol. 48 (1987) 249-254
2. Wiemken A. Trehalose in yeast, stress protectant rather than reserves carbohydrate. J. Gen. Mol. Microbiol. 58 (1990) 209-217
3. Strom A.R., and Kaasen I. Trehalose metabolism in Escherichia coli: stress protection and stress regulation of gene expression. Mol. Microbiol. 8 (1993) 205-210
4. Goddijn O., and van Dun K. Trehalose metabolism in plants. Trends Plant Sci. 4 (1999) 315-319
5. Elbein A.D., Pan Y.T., Pastuszak I., and Carrol D. New insights on trehalose: a multifunctional molecule. Glycobiology 13 (2003) 17R-27R
6. Adams R.P., Kendall E., and Kartha K.K. Comparison of free sugars in growing and desiccated plants of Selaginella lepidophylla. Biochem. Syst. Ecol. 18 (1990) 107-110
7. Drennan P.M., Smith M.T., Goldsworthy D., and van Staden J. The occurrence of trehalose in the leaves of the desiccation-tolerant angiosperm Myothamnus flabellifolius Welw. J. Plant Physiol. 143 (1993) 493-496
8. Cabib E., and Leloir L.F. The biosynthesis of trehalose phosphate. J. Biol. Chem. 231 (1958) 259-275
9. Leyman B., Van Dijck P., and Thevelein J.M. An unexpected plethora of trehalose biosynthesis genes in Arabidopsis thaliana. Trends Plant Sci. 6 (2001) 510-513
10. Blazquez M.A., Santos E., Flores C.L., Martínez-Zapater J.M., Salinas J., and Gancedo C. Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase. Plant J. 13 (1998) 685-690
11. Eastmond P.J., van Dijken A.J.H., Spielman M., Kerr A., Tissier A.F., Dickinson H.G., Jones J.D.G., Smeekens S.C., and Graham I.A. Trehalose-6-phosphate synthase 1, which catalyses the first step in trehalose synthesis, is essential for Arabidopsis embryo maturation. Plant J. 29 (2002) 225-235
12. van Dijken A., Schluepmann H., and Smeekens S.C.M. Arabidopsis trehalose-6-phosphate synthase 1 is essential for normal vegetative growth and transition to flowering. Plant Physiol. 135 (2004) 969-977
13. Thevelein J.M., and Hohmann S. Trehalose synthase: guard to the gate of glycolysis in yeast?. Trends Biochem. 20 (1995) 3-10
14. Schluepmann H., Pellny T., van Dijken A., Smeekens S., and Paul M. Trehalose 6-phosphate is indispensable for carbohydrate utilization and growth in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 100 (2003) 6849-6854
15. Schluepmann H., van Dijken A., Aghdasi M., Wobbes B., Paul M., and Smeekens S. Trehalose mediated growth inhibition of Arabidopsis seedlings is due to trehalose-6-phosphate accumulation. Plant Physiol. 135 (2004) 879-890
16. Avonce N., Leyman B., Mascorro-Gallardo J.O., Van Dijck P., Thevelein J.M., and Iturriaga G. The Arabidopsis trehalose-6-phosphate synthase AtTPS1 gene is a regulator of glucose, abscisic acid, and stress signaling. Plant Physiol. 136 (2004) 3649-3659
17. Kolbe A., Tiessen A., Schluepmann H., Paul M., Ulrich S., and Geingenberger P. Trehalose 6-phosphate regulates starch synthesis via posttranslational redox activation of ADP-glucose pyrophosphorylase. Proc. Natl. Acad. Sci. USA 102 (2005) 11118-11123
18. Londesborough J., and Vuorio O.E. Purification of trehalose synthase from baker's yeast. Its temperature-dependent activation by fructose-6-phosphate and inhibition by phosphate. Eur. J. Biochem. 216 (1993) 841-848
19. Pan Y.T., Carrol J.D., and Elbein A.D. Trehalose-phosphate synthase of Mycobacterium tuberculosis. Cloning, expression and properties of the recombinant enzyme. Eur. J. Biochem. 269 (2002) 6091-6100
20. Pan Y.T., Korot Edavana V., Jourdian W.J., Edmonson R., Carrol J.D., Pastuszak I., and Elbein A.D. Trehalose synthase of Mycobacterium smegmatis: purification, cloning, expression and properties of the enzyme. Eur. J. Biochem. 271 (2004) 4259-4269
21. Valenzuela-Soto E.M., Márquez-Escalante J.A., Iturriaga G., and Figueroa-Soto C.G. Trehalose 6-phosphate synthase from Selaginella lepidophylla: purification and properties. Biochem. Biophys. Res. Commun. 313 (2004) 314-319
22. Fiol D.F., and Salerno G.L. Trehalose synthesis in Euglena gracilis (Euglenophyceae) occurs through an enzyme complex. J. Phycol. 41 (2005) 812-818
23. Vandercammen A., François J., and Henri-Gery H. Characterization of trehalose-6-phosphate synthase and trehalose-6-phosphate phosphatase of Saccharomyces cerevisiae. Eur. J. Biochem. 182 (1989) 613-620
24. Bell W., Klaassen P., Ohnacker M., Boller T., Herweijer M., Schoppink P., Van der Zee P., and Wiemken A. Characterization of the 56-kDa subunit of yeast trehalose-6-phosphate synthase and cloning of its gene reveal its identity with the product of CIF1, a regulator of carbon catabolite inactivation. Eur. J. Biochem. 209 (1992) 951-959
25. Londesborough J., and Vuorio O.E. Trahlose-6-phosphate synthase/phosphatase complex from baker's yeast: purification of a proteolytically activated form. J. Gen. Microbiol. 137 (1991) 841-848
26. Vuorio O.E., Kalkkinen N., and Londesborough J. Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae. Eur. J. Biochem. 216 (1993) 849-861
27. de Virgilio C., Bürckert N., Bell W., Jenö P., Boller T., and Wiemken A. Disruption of TPS2, the gene encoding the 100-kDa subunit of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae, causes accumulation of trehalose-6-phosphate and loss of trehalose-6-phosphate activity. Eur. J. Biochem. 212 (1993) 315-323
28. Bell W., Sun W., Hohmann S., Wera S., Reinders A., de Virgilio C., Wiemken A., and Thevelein J.M. Composition and functional analysis of the Saccharomyces cerevisiae trehalose synthase complex. J. Biol. Chem. 273 (1998) 33311-33319
29. Giaever H.M., Styrvold O.B., Kaasen I., and Strom A.R. Biochemical and genetic characterization of osmoregulatory trehalose synthesis in Escherichia coli. J. Bacteriol. 170 (1988) 2841-2849
30. Kaasen I., McDougall J., and Strom A.R. Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex. Gene 145 (1994) 9-15
31. Zentella R., Mascorro-Gallardo J.O., Van Diijck P., Folch-Mallol J., Bonini B., Van Vaeck C., Gaxiola R., Covarrubias A.A., Nieto-Sotelo J., Thevelein J.M., and Iturriaga G. Sellaginella lepidophyalla trehalosa-6-phosphate synthase complements growth and stress-tolerance defects in a yeast tps1 mutant. Plant Physiol. 119 (1999) 1473-1482
32. Hottiger T., Schmutz P., and Wiemken A. Heat-induced accumulation and futile cycling of trehalose in Saccharomyces cerevisiae. J. Bacteriol. 169 (1987) 5518-5522
33. Bradford M.M. A rapid and sensitive method for the quantitation of micrograms of protein utilizing the principle of protein-binding. Anal. Biochem. 72 (1976) 248-254
34. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 277 (1976) 680-685
35. Wray W., Boulikas T., Wray V.P., and Hancock R. Silver staining of proteins in polyacrilamide gels. Anal. Biochem. 118 (1981) 197-203
36. Killick K.A. Trehalose 6-phosphate synthase from Dictyostelium discoideum: partial purification and characterization of the enzyme from young sorocarps. Arch. Biochem. Biophys. 196 (1979) 121-133
37. Nishimoto T., Nakada T., Chaen H., Fukuda S., Sugimoto T., Kurimoto M., and Tsujisaka Y. Purification and characterization of a thermostable trehalose synthase from Thermus aquaticus. Biosci. Biotechnol. Biochem. 60 (1996) 835-839
38. Nishimoto T., Nakada T., Chaen H., Fukuda S., Sugimoto T., Kurimoto M., and Tsujisaka Y. Purification and properties of a novel enzyme, trehalose synthase, from Pimelobacter sp. R48. Biosci. Biotechnol. Biochem. 60 (1996) 640-644