Trehalose synthase of Mycobacterium smegmatis: Purification, cloning, expression, and properties of the enzyme

European Journal of Biochemistry

Volumn 271, Issue 21, 2004, Pages 4259-4269

  Pan, Yuan T ^a   Edavana, Vineetha Koroth ^a   Jourdian, William J ^b   Edmondson, Rick ^c   Carroll, J David ^a   Pastuszak, Irena ^a   Elbein, Alan D ^a  

^a UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

^b UNIVERSITY OF MICHIGAN HEALTH SYSTEM   (United States)

^c NATIONAL CENTER FOR TOXICOLOGICAL RESEARCH   (United States)

Author keywords

Maltose; Mycobacteria; Sugar interconversions; Trehalose biosynthesis; Trehalose metabolism

Indexed keywords

CARBOHYDRATE; DISACCHARIDE; GLUCOSE; MALTOSE; SYNTHETASE; TREHALOSE; TREHALOSE SYNTHASE; UNCLASSIFIED DRUG;

ARTICLE; BIOSYNTHESIS; CATALYSIS; CONCENTRATION (PARAMETERS); CYTOSOL; ENZYME PURIFICATION; ENZYME SUBUNIT; ESCHERICHIA COLI; GENE IDENTIFICATION; GENE SEQUENCE; MOLECULAR CLONING; MOLECULAR WEIGHT; MYCOBACTERIUM SMEGMATIS; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION;

AMINO ACID SEQUENCE; AMMONIUM SULFATE; BINDING SITES; CARBOHYDRATES; CATALYSIS; CELLULOSE; CHROMATOGRAPHY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, ION EXCHANGE; CLONING, MOLECULAR; CYTOSOL; DATABASES; DISACCHARIDES; ENZYME INHIBITORS; ESCHERICHIA COLI; GLUCOSE; GLUCOSYLTRANSFERASES; HYDROGEN-ION CONCENTRATION; KINETICS; MALTOSE; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM SMEGMATIS; OPEN READING FRAMES; PEPTIDES; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SODIUM DODECYL SULFATE; SUBSTRATE SPECIFICITY; TIME FACTORS; TREHALOSE;

ACTINOBACTERIA (CLASS); BACTERIA (MICROORGANISMS); CORYNEBACTERINEAE; ESCHERICHIA COLI; MYCOBACTERIUM; MYCOBACTERIUM SMEGMATIS; UNCULTURED ACTINOMYCETE;

EID: 8644245771     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.2004.04365.x     Document Type: Article

References (30)

1. Elbein, A.D., Pan, Y.T., Pastuszak, I. & Carroll, J.D. (2003) New insights on trehalose: a multifunctional molecule. Glycobiology 13, 17R-27R.
2. Trevelyan, W.E. & Harrison, J.S. (1956) Studies on yeast metabolism. The trehalose content of baker's yeast during anaerobic fermentation. Biochem. J. 62, 177-182.
3. Nwaka, S. & Holzer, H. (1998) Molecular biology of trehalose and trehalases in the yeast, Saccharomyces cerevesiae. Prog. Nucleic Acid Res. Mol. Biol. 58, 197-237.
4. Elbein, A.D. (1974) The metabolism of α,α-trehalose. Adv. Carbohydrate Chem. Biochem. 30, 227-256.
5. Brennan, P.J. & Nikaido, H. (1995) The envelope of mycobacteria. Annu. Rev. Biochem. 64, 29-63.
6. Shimakata, T. & Minatagawa, Y. (2000) Essential role of trehalose in the synthesis and subsequent metabolism of corynomycolic acid in Corynebacterium matruchotii. Arch. Biochem. Biophys. 380, 331-338.
7. Liu, J. & Nikaido, H. (1999) A mutant of Mycobacterium smegmatis defective in the biosynthesis of mycolic acid accumulates meromycolate. Proc. Natl Acad. Sci. USA 96, 4011-4016.
8. De Virgilio, C., Hottinger, T., Dominiguez, J., Boller, T. & Wiemken, A. (1994) The role of trehalose synthesis for the acquisition of thermotolerance in yeast. Eur. J. Biochem. 219, 179-186.
9. Hounsa, C.-G., Brandt, E.V., Trevelyan, J., Hohmann, S. & Prior, B.A. (1998) Role of trehalose in survival of Saccharomyces cerevesiae under osmotic stress. Microbiology 144, 671-680.
10. Noubhani, A., Bunoust, O., Rigolet, M. & Thevelein, J.M. (2000) Reconstitution of ethanolic fermentation in permeabilized spheroblasts of wild-type and trehalose-6-phosphate synthase mutants of the yeast, Saccharomyces cerevesiae. Eur. J. Biochem. 267, 4566-4576.
11. Muller, J., Wiemken, A. & Aeschbacher, R. (1999) Trehalose metabolism in sugar sensing and plant development. Plant Sci. 147, 37-47.
12. Muller, J., Aeschbacher, R.A., Wingler, A., Boller, T. & Wiemken, A. (2001) Trehalose and trehalase in Arabidopsis. Plant Physiol. 125, 1086-1093.
13. Nwaka, S., Mechler, B., Destruelle, M. & Holzer, H. (1995) Phenotypic features of trehalose mutants in Saccharomyces cerevesiae. FEBS Lett. 360, 286-290.
14. De Smet, K.A.L., Weston, A., Brown, I.N., Young, D.B. & Robertson, B.D. (2000) Three pathways for trehalose biosynthesis in mycobacteria. Microbiology 146, 199-208.
15. Cabib, E. & Leloir, L.F. (1958) The biosynthesis of trehalose-phosphate. J. Biol. Chem. 231, 259-275.
16. Matula, M., Mitchell, M. & Elbein, A.D. (1971) Partial purification of a highly specific trehalose-phosphate phosphatase from Mycobacterium smegmatis. J. Bacteriol. 107, 217-223.
17. Nishimoto, T., Nakano, M., Nakada, T., Chaen, H., Fukuda, S., Sugimoto, T., Kurimoto, M. & Tsujisaka, Y. (1995) Purification and properties of a novel enzyme, trehalose synthase, from Pimelobacter sp. R48. Biosci. Biotechnol. Biochem. 60, 640-644.
18. Maruta, K., Mitsuzumi, H., Nakada, T., Kubota, M., Chaen, H., Fukuda, S., Sugimoto, T. & Kurimoto, M. (1996) Cloning and sequencing of a cluster of genes encoding novel enzymes of trehalose biosynthesis from thermophilic archaebacterium Sulfolobus acidocaldarius. Biochim. Biophys. Acta 1291, 177-181.
19. Cole, S., Brosch, R., Parkhill, J., Garnier, T., Churcher, C., Harris, D., Gordon, S.V., Eiglmeier, K., Gas, S., Barry, C.E., Tekaia, F., Badcock, K., Basham, D., Brown, D., Chillingworth, T., Connor, R., Davies, R., Devlin, K., Feltwell, T., Gentles, S., Hamlin, N., Holyord, S., Hornsby, T., Jagels, K., Krogh, A., McLean, J., Moule, S., Murphy, L., Oliver, K., Osborne, J., Quail, M.A., Rajandream, M.A., Rogers, J., Rutter, S., Seeger, K., Skelton, J., Squares, R., Squares, S., Sulston, I.E., Taylor, K., Whitehead, S. & Barrell, B.G. (1998) Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393, 537-544.
20. Pan, Y.T., Drake, R.R. & Elbein, A.D. (1996) Trehalose-P synthase of mycobacteria: Its substrate specificity is affected by polyanions. Glycobiology 6, 453-461.
21. Nelson, N. (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol. Chem. 153, 375-3280.
22. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
23. Lederer, E. (1976) Cord factor and related trehalose esters. Chem. Phys. Lipids 16, 91-106.
24. Thevelein, J.M. (1984) Regulation of trehalose metabolism in fungi. Microbiol. Rev. 48, 42-59.
25. Iwahashi, H., Obuchi, K., Fujii, S. & Komatsu, Y. (1997) Effect of temperature on the role of Hsp104 and trehalose in barotolerance of Saccharomyces cerevesiae. FEBS Lett. 416, 1-5.
26. Pan, Y.T., Carroll, J.D. & Elbein, A.D. (2002) Trehalose-phosphate synthase of Mycobacterium tuberculosis: Cloning, expression and properties of the recombinant enzyme. Eur. J. Biochem. 269, 6091-6100.
27. Klutts, S., Pastuszak, I., Edavana, V.K., Thampi, P., Pan, Y.T., Abraham, E.C., Carroll, J.D. & Elbein, A.D. (2003) Purification, cloning, expression and properties of mycobacterial trehalose-phosphate phosphatase. J. Biol. Chem. 278, 2093-2100.
28. Pan, Y.T. & Elbein, A.D. (1996) Inhibition of the trehalose-P synthase of mycobacteria by various antibiotics. Arch. Biochem. Biophys. 335, 258-266.
29. Wolf, A., Kramer, R. & Marbach, S. (2003) Three pathways for trehalose metabolism in Corynebacterium glutamicum ATCC 13032, and their significance in response to osmotic stress. Mol. Microbiol. 49, 1119-1134.
30. Tzetkov, M., Klopprogge, C., Zelder, O. & Liebl, W. (2003) Genetic dissection of trehalose biosynthesis in Corynebacterium glutamicum: Inactivation of trehalose production leads to impaired growth and an altered cell wall lipid composition. Microbiology 149, 1659-1673.