Development of a real time PCR for the detection of Taylorella equigenitalis directly from genital swabs and discrimination from Taylorella asinigenitalis

Veterinary Microbiology

Volumn 118, Issue 3-4, 2006, Pages 247-254

  Wakeley, P R ^a   Errington, J ^a   Hannon, S ^a   Roest, H I J ^b   Carson, T ^a   Hunt, B ^a   Sawyer, J ^a   Heath, P ^a  

^a VETERINARY LABORATORIES AGENCY   (United Kingdom)

^b WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Contagious equine metritis; Direct PCR amplification; Taylorella asinigenitalis; Taylorella equigenitalis

Indexed keywords

BACTERIAL DNA; DNA 16S; DNA FRAGMENT;

AMPLICON; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; CONTAGIOUS EQUINE METRITIS; CROSS HYBRIDIZATION; DNA EXTRACTION; FEMALE; GENE AMPLIFICATION; MALE; MEDICAL INSTRUMENTATION; METRITIS; NETHERLANDS; NONHUMAN; NUCLEOTIDE SEQUENCE; REAL TIME POLYMERASE CHAIN REACTION; TAYLORELLA; TAYLORELLA ASINIGENITALIS; TAYLORELLA EQUIGENITALIS;

ANIMALS; BASE SEQUENCE; COLONY COUNT, MICROBIAL; DIAGNOSIS, DIFFERENTIAL; DNA, BACTERIAL; DNA, RIBOSOMAL; ENDOMETRITIS; FEMALE; GENE AMPLIFICATION; GRAM-NEGATIVE BACTERIAL INFECTIONS; HORSE DISEASES; HORSES; PHYLOGENY; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; SPECIES SPECIFICITY; TAYLORELLA; TAYLORELLA EQUIGENITALIS;

BACTERIA (MICROORGANISMS); EQUIDAE; TAYLORELLA; TAYLORELLA ASINIGENITALIS; TAYLORELLA EQUIGENITALIS;

EID: 33750992184     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2006.08.007     Document Type: Article

References (21)

1. Anonymous. Third case of CEM confirmed. Vet. Rec. 152 (2003) 311
2. Anzai T., Eguchi M., Sekizaki T., Kamada M., Yamamoto K., and Okuda T. Development of a PCR test for rapid diagnosis of contagious equine metritis. J. Vet. Med. Sci. 61 (1999) 1287-1292
3. Arata A.B., Cooke C.L., Jang S.S., and Hirsh D.C. Multiplex polymerase chain reaction for distinguishing Taylorella equigenitalis from Taylorella equigenitalis-like organisms. J. Vet. Diagn. Invest. 13 (2001) 263-264
4. Atherton J.G. Evaluation of selective supplements used in media for the isolation of the causative organism of contagious equine metritis. Vet. Rec. 113 (1983) 299-300
5. Bleumink-Pluym N.M., van Dijk L., van Vliet A.H., van der Giessen J.W., and van der Zeijst B.A. Phylogenetic position of Taylorella equigenitalis determined by analysis of amplified 16S ribosomal DNA sequences. Int. J. Syst. Bacteriol. 43 (1993) 618-621
6. Bleumink-Pluym N.M., Werdler M.E., Houwers D.J., Parlevliet J.M., Colenbrander B., and van der Zeijst B.A. Development and evaluation of PCR test for detection of Taylorella equigenitalis. J. Clin. Microbiol. 32 (1994) 893-896
7. Crowhurst R.C., Simpson D.J., Greenwood R.E., and Ellis D.R. Contagious equine metritis. Vet. Rec. 104 (1979) 465
8. Fernie D.S., Batty I., Walker P.D., Platt H., Mackintosh M.E., and Simpson D.J. Observations on vaccine and post-infection immunity in contagious equine metritis. Res. Vet. Sci. 28 (1980) 362-367
9. Jackson G., Carson T., Heath P., and Cooke G. CEMO in a UK stallion. Vet. Rec. 151 (2002) 582
10. Jang S.S., Donahue J.M., Arata A.B., Goris J., Hansen L.M., Earley D.L., Vandamme P.A., Timoney P.J., and Hirsh D.C. Taylorella asinigenitalis sp. nov., a bacterium isolated from the genital tract of male donkeys (Equus asinus). Int. J. Syst. Evol. Microbiol. 51 (2001) 971-976
11. Mitsuhashi M. Technical report. Part 2. Basic requirements for designing optimal PCR primers. J. Clin. Lab. Anal. 10 (1996) 285-293
12. Moore J.E., Millar B.C., and Buckley T.C. Potential misidentification of Taylorella asinigenitalis as Taylorella equigenitalis: implications for the epidemiology of CEM. J. Equine Sci. 20 (2000) 479
13. Moore J.E., Millar B.C., Xu J., and Buckley T.C. Possible misidentification of Bacteroides sp., probably B. ureolyticus as Taylorella equigenitalis: implications for the laboratory diagnosis of CEM. Vet. Res. 32 (2001) 611-616
14. Powell D.G. Contagious equine metritis. Adv. Vet. Sci. Comp. Med. 25 (1981) 161-184
15. Premanandh J., George L.V., Wernery U., and Sasse J. Evaluation of a newly developed real-time PCR for the detection of Taylorella equigenitalis and discrimination from T. asinigenitalis. Vet. Microbiol. 95 (2003) 229-237
16. Rideout M.I., Burns S.J., and Simpson R.B. Influence of bacterial products on the motility of stallion spermatozoa. J. Reprod. Fertil. Suppl. 32 (1982) 35-40
17. Rupf S., Merte K., and Eschrich K. Quantification of bacteria in oral samples by competitive polymerase chain reaction. J. Dent. Res. 78 (1999) 850-856
18. Schluter H., Kuller H., Friedreich U., Selbitz H., Marwitz T., Beyer C., and Ullrich E. Epizootiology and treatment of contagious equine metritis (CEM), with particular reference to the treatment of infected stallions. Prakt. Tierarzt. 72 (1991) 503-511
19. Sugimoto C., Isayama Y., Sakazaki R., and Kuramochi S. Transfer of Haemophilus equigenitalis Taylor et al., 1978 to the genus Taylorella gen. nov. as Taylorella equigenitalis comb.nov. Curr. Microbiol. 9 (1983) 155-162
20. Swerczek T.W. Inhibition of the CEM organism by the normal flora of the reproductive tract. Vet. Rec. 103 (1978) 125
21. Timoney P.J., Shin S.J., and Jacobson R.H. Improved selective medium for isolation of the contagious equine metritis organism. Vet. Rec. 111 (1982) 107-108