Development of a PCR Test for Rapid Diagnosis of Contagious Equine Metritis

Journal of Veterinary Medical Science

Volumn 61, Issue 12, 1999, Pages 1287-1292

  Anzai, Toru ^a   Eguchi, Masashi ^b   Sekizaki, Tsutomu ^b   Kamada, Masanobu ^a   Yamamoto, Kohshi ^b   Okuda, Toshio ^c  

^a EQUINE RESEARCH INSTITUTE   (Japan)

^b NATIONAL INSTITUTE OF ANIMAL HEALTH   (Japan)

^c Hidaka Livestock Hyg Service Center   (Japan)

Author keywords

Contagious equine metritis; Equine; Metritis; Polymerase chain reaction; Taylorella equigenitalis

Indexed keywords

ANIMALIA; BACTERIA (MICROORGANISMS); EQUIDAE; OLIGELLA URETHRALIS; TAYLORELLA EQUIGENITALIS;

BACTERIAL DNA; PRIMER DNA;

AGAR GEL ELECTROPHORESIS; ANIMAL; ANIMAL DISEASE; ARTICLE; CHEMISTRY; CLITORIS; DNA SEQUENCE; ENDOMETRITIS; FEMALE; GENE LIBRARY; GENETICS; GRAM NEGATIVE INFECTION; HORSE; HORSE DISEASE; ISOLATION AND PURIFICATION; MALE; METHODOLOGY; MICROBIOLOGY; MOLECULAR GENETICS; NUCLEIC ACID HYBRIDIZATION; NUCLEOTIDE SEQUENCE; PENIS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TAYLORELLA EQUIGENITALIS; UTERINE CERVIX;

ANIMALS; BASE SEQUENCE; CERVIX UTERI; CLITORIS; DNA PRIMERS; DNA, BACTERIAL; ELECTROPHORESIS, AGAR GEL; ENDOMETRITIS; FEMALE; GENE LIBRARY; GRAM-NEGATIVE BACTERIAL INFECTIONS; HORSE DISEASES; HORSES; MALE; MOLECULAR SEQUENCE DATA; NUCLEIC ACID HYBRIDIZATION; PENIS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; TAYLORELLA EQUIGENITALIS;

EID: 0033254179     PISSN: 7653697     EISSN: 1782155X     Source Type: Journal    
DOI: 10.1292/jvms.61.1287     Document Type: Article

References (25)

1. Birnboim, H. C. and Doly, J. 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7: 1513-1523.
2. Bleumink-Pluym, N.E.A. ter Laak and van der Zeijst, B.A.M. 1990. Epidemiologic study of Taylorella equigenitalis strains by field inversion gel electrophoresis of genomic restriction endonuclease fragments. J. Clin. Microbiol. 28: 2012-2016.
3. Bleumink-Pluym, N. M. C., Werdler, M. E. B., Houwers, D. J., Parlevliet, J. M., Colenbrander, B. and Van der Zeijst, B. A. M. 1994. Development and evaluation of PCR test for detection Taylorella equigenitalis. J. Clin. Microbiol. 32: 893-896.
4. Bøvre, K. and Hagen, N. 1981. The family Neisseriaceae: rod-shaped species of the genera Moraxella, Acinetobacter, Kingella, and the Branhamella group of cocci, pp. 1506-1529. In: The Prokaryotes ( Starr, M.P., Stolp, H., Truper, H.G., Balow, A. and Schlegel, H.G. eds.), Springer-Verlag, Berlin.
5. Bøvre, K. 1979. Proposal to divide the genus Moraxella Lwoff 1939 emend. Henriksen and Bvre 1968 into two subgenera, subgenus Moraxella (Lwoff 1939) Bovre 1979 and subgenus Branhamella (Catlin 1970) Bovre 1979. Int. J. Syst. Bacteriol. 29: 403-406.
6. Chanter N., Vigano, F., Collon, N. C. and Mumford, J. A. 1998. Use of a PCR assay for Taylorella equigenitalis applied to samples from the United Kingdom. Vet. Rec. 22: 225-227.
7. Crowhurst, R. C. 1977. Genital infection in mares. Vet. Rec. 100: 476.
8. Eguchi, M., Kuniyasu, C. and Kishima, M. 1988. Passive hemagglutination test for detection of antibodies against Taylorella (Haemophilus) equigenitalis in sera of mares. Vet. Microbiol. 18: 155-161.
9. Ezaki, T. and Suzuki, S. 1982. Achromopeptidase for lysis of anaerobic gram-positive cocci. J. Clin. Microbiol. 16: 844-846.
10. Kamada, M., Akiyama, Y., Oda, T. and Fukuzawa, Y. 1981. Contagious equine metritis: Isolation of Haemophilus equigenitalis from horses with endometritis in Japan. Jpn. J. Vet. Sci. 43: 565-568.
11. Kamada, M., Anzai, T., Kanemaru, T., Wada, R. and Kumanomido, T. 1987. Contagious equine metritis: Characterization of small and large colonial variants of Taylorella equigenitalis isolated from a laboratory strain. Bull. Equine Res. Inst. 24: 23-32.
12. Kamada, M., Kumanomido, T., Anzai, T., Kanemaru, T., Senba, H. and Ohishi, H. 1986. Isolation and drug susceptibility of streptomycin sensitive Taylorella equigenitalis from mares with metritis and infertility in Japan. Bull. Equine Res. Inst. 23: 55-61.
13. Miserez, R., Frey, J., Krawinkeler, M. and Nicolet, J. 1996. Identification and diagnosis of Taylorella equigenitalis by a method of DNA-amplification (PCR). Schweiz. Arch. Tierheilk. 138: 115-120.
14. Osaki, M., Sato, Y., Tomura, H., Ito, H. and Sekizaki, T. 1997. Genetic diversity of the genes encoding the outer membrane lipoprotein (omlA) of Actinobacillus pleuropneumoniae. J. Vet. Med. Sci. 59: 213-215.
15. Powell, D. G. 1981. Contagious equine metritis. Adv. Vet. Sci. Comp. 25: 161-184.
16. Rossau, R., Kersters, K., Falsen, E., Jantzen, E., Segers, P. Union, A., Nehls, L. and De Ley, J. 1987. Oligella, a new genus including Oligella urethralis comb. nov. (formerly Moraxella urethralis) and Oligella ureolytica sp. nov. (formerly CDC group IVe): relationship to Taylorella equigenitalis and related taxa. Int. J. Syst. Bacteriol. 37: 198-210.
17. Sahu, S. P., Wool, S. and Breese, S. S. Jr. 1982. Observation on the morphology of contagious equine metritis bacterial colonies isolated from infected pony mares. Am. J. Vet. Res. 43: 796-800.
18. Sambrook, J., Fritsch E.F. and Maniatis, T. 1989. Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
19. Sanger, F., Nicklen, S. and Coulsen, A. R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74: 5463-5467.
20. Sekizaki, T., Ito, H., Asawa, T. and Nonomura, I. 1993. DNA sequence of type 1 fimbirn, Fpu11, gene from a chicken pathogenic Escherichia coli serotype O78. J. Vet. Med. Sci. 55: 395-400.
21. Swaney, L. M. and Sau, S. P. 1978. CEM: Bacteriological methods. Vet. Rec. 102: 43.
22. Swerczek, T. W. 1978. Contagious equine metritis in the USA. Vet. Rec. 102: 512-513.
23. Timoney, P. J., Ward, J. and Kelly, P. 1977. A contagious genital infection of mares. Vet. Rec. 101: 103.
24. Wada, R., Kamada, M., Fukunaga, Y. and Kumanomido, T. 1983. Studies on contagious equine metritis IV. Pathology in horses experimentally infected with Haemophilus equigenitalis. Bull. Equine Res. Inst. 20: 133-143.
25. Watson, E. D. 1997. Swabbing protocols in screening for contagious equine metritis. Vet. Rec. 140: 268-271.