Establishment and validation of Caco-2 cell lines for intestinal epithelial permeability

Chinese Pharmaceutical Journal

Volumn 41, Issue 18, 2006, Pages 1431-1434

  Sun, Min Jie ^a   Sheng, Xing ^a   Hu, Yi Qiao ^a  

^a NANJING UNIVERSITY   (China)

Author keywords

Caco 2 cell; Permeability; Transepithelial electrical resistance(TEER); Transport

Indexed keywords

ALKALINE PHOSPHATASE; FLUORESCEIN SODIUM;

ARTICLE; CELL CULTURE; CELL DENSITY; CELL GROWTH; CELL MEMBRANE PERMEABILITY; CELL STRAIN CACO 2; CONTROLLED STUDY; HUMAN; HUMAN CELL; INTESTINE EPITHELIUM CELL; MICROVILLUS; MONOLAYER CULTURE; MORPHOLOGY;

EID: 33750956011     PISSN: 10012494     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (8)

1. YEE S. In vitro permeability across Caco-2 cells(colonic) can predict in vivo (small intestinal) absorption[J]. Pharm Res, 1997, 14(6): 763-766.
2. ALLEN R H, ROBERT A C, PHILLIP S B. Caco-2 cell monolayers as a model for drug transport across the intestinal mucosa[J]. Pharm Res, 1990, 7(9):902-910.
3. GAN L L, DHIREN R T. Application of the Caco-2 models in the design and development of orally active drugs: elucidation of biochemical and physical barriers posed by the intestinal epithelium[J]. Adv Drug Deliv Rev, 1997, 23(1):77-98.
4. PATRICK A, RAF M. HPLC with programmed wavelength fluorescence detection for the simultaneous determination of marker compounds of integrity and P-gp functionality in the Caco-2 intestinal absorption model[J]. J Pharm Biomed Anal, 2004, 34(5): 971-978.
5. JULIE J, MICHEL D, RICHARD B L. Inhibition of the multidrug resistance P-glycoprotein activity by green tea polyphenols[J]. Biochim Biophys Acta, 2002, 1542(1-3):149.
6. CAI W Q. Modern Useful Manual of Cell and Molecular Biology Techniques [M]. 1st ed. Beijing: People's Military Medical Publisher, 2003:222-229.
7. PRAVEEN V B, KARISHMA P. Utility of 96 well Caco-2 cell system for increased throughput of P-gp screening in drug discovery[J]. Eur J Pharm & Biopharm, 2004, 58(1): 99-105.
8. HIDALGO I J, RAUB T J, BORCHARDT R T. Characterizaton of the human colon adenocarcinoma cell lines(Caco-2) as a model system for intestinal epithelial permeability[J]. Gastroenterology, 1989, 96(3): 736-749.