Rapid isocratic HPLC quantification of metallothionein-like proteins as biomarkers for cadmium exposure in the earthworm Eisenia andrei

Soil Biology and Biochemistry

Volumn 39, Issue 1, 2007, Pages 194-201

  Ndayibagira, Aristocle ^a   Sunahara, Geoffrey I ^a   Yves Robidoux, Pierre ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

Biomarker; Earthworm; Heavy metals; Metalloprotein; SBD F; UV absorption

Indexed keywords

BIODIVERSITY; CADMIUM; CONTAMINATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PROTEINS; SOILS; ULTRAVIOLET RADIATION;

EARTHWORMS; METALLOPROTEINS; METALLOTHIONEINS (MT); RETENTION TIME; UV ABSORPTION;

BIOMARKERS;

BIOMARKER; CADMIUM; EARTHWORM; LIQUID CHROMATOGRAPHY; METALLOTHIONEIN; SOIL POLLUTION; ULTRAVIOLET RADIATION;

EISENIA ANDREI; ORYCTOLAGUS CUNICULUS;

EID: 33750945706     PISSN: 00380717     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.soilbio.2006.07.008     Document Type: Article

References (37)

1. Berger B., Dallinger R., and Thomaser A. Quantification of metallothionein as a biomarker for Cd exposure in terrestrial gastropods. Environmental Toxicology and Chemistry 14 (1995) 781-791
2. Bracken W.M., and Klaassen C.D. Induction of metallothionein by steroids in rat primary hepatocyte cultures. Toxicology and Applied Pharmacology 87 (1987) 381-388
3. Bradford M.M. A rapid and sensitive method for the quantification of microgram quantities of proteins utilizing the principle of protein-dye binding. Analytical Biochemistry 72 (1976) 248-254
4. Bragigand V., and Berthet B. Some methodological aspects of metallothionein evaluation. Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology 134 (2003) 55-61
5. Coyle P., Philcox J.C., Carey L.C., and Rofe A.M. Metallothionein: the multipurpose protein. Cellular and Molecular Life Sciences 59 (2002) 627-647
6. Dallinger R. Metallothionein research in terrestrial invertebrates: synopsis and perspectives. Comparative Biochemistry and Physiology C: Pharmacology, Toxicology and Endocrinology 113 (1996) 125-133
7. Dallinger R., Berger B., Gruber C., Hunziker P.E., and Stürzenbaum S. Metallothionein in terrestrial invertebrates: structural aspects, biological significance and implications for their use as biomarkers. Cellular and Molecular Biology 46 (2000) 331-346
8. Dallinger R., Chabocovsky M., and Berger B. Isoform-specific quantification of metallothionein in the terrestrial gastropod Helix pomatia I. Molecular, biochemical, and methodological background. Environmental Toxicology and Chemistry 23 (2004) 890-901
9. Furst A., and Nguyen Q. Cadmium-induced metallothionein in earthworms (Lumbricus terrestris). Biological Trace Elements Research 21 (1989) 81-85
10. Galay Burgos M., Winters C., Stürzenbaum S.R., Randerson P.F., Kille P., and Morgan A.J. Cu and Cd on the earthworm Lumbricus rubellus in the laboratory: multivariate statistical analysis of relationships between exposure, biomarkers, and ecologically relevant parameters. Environmental Science & Technology 39 (2005) 1757-1763
11. Ghazi I.E., Menge S., Miersch J., Chafik A., Benhra A., Elamrani M.K., and Krauss G.J. Quantification of metallothionein-like proteins in the mussel Mytilus galloprovincialis using RP-HPLC fluorescence detection. Environmental Science & Technology 37 (2004) 5739-5744
12. Gillis P.L., Diener L.C., Reynoldson T.B., and Dixon D.G. Cadmium-induced production of a metallothioneinlike protein in Tubifex tubifex (Oligocheata) and Chironomus riparius (Diptera): correlation with reproduction and growth. Environmental Toxicology and Chemistry 21 (2002) 1836-1844
13. Gruber C., Stürzenbaum S.R., Gehrig P., Sack R., Hunziker P., Berger B., and Dallinger R. Isolation and characterization of a self-sufficient one-domain protein (Cd)-Metallothionein from Eisenia foetida. European Journal of Biochemistry 267 (2000) 573-582
14. Haase H., and Maret W. A differential assay for the reduced and oxidized states of metallothionein and thionein. Analytical Biochemistry 333 (2004) 19-26
15. ISO: International Organization for Standardization, 1996. Soil quality-effects of pollutants on earthworms (Eisenia fetida). Part 2: determination of effects on reproduction, No.11268-2. ISO, Geneve.
16. Kammenga J.E., Dallinger R., Donker M.H., Kohler H.R., Simonsen V., Triebskorn R., and Weeks J.M. Biomarkers in terrestrial invertebrates for ecotoxicological soil risk assessment. Reviews of Environmental Contamination and Toxicology 164 (2000) 93-147
17. Klaassen C.D., Liu J., and Choudhuri S. Metallothionein: an intracellular protein to protect against cadmium toxicity. Annual Reviews in Phamacology and Toxicology 39 (1999) 267-294
18. Klaverkamp J.F., Wautier K., and Baron C.L. A modified mercury saturation assay for measuring metallothionein. Aquatic Toxicology 50 (2000) 13-25
19. Miyairi S., and Naganuma A. Metallothionein determination by isocratic HPLC with fluorescence derivatization. Methods in Molecular Biology 186 (2002) 273-283
20. Miyairi S., Shibata S., and Naganuma A. Determination of metallothionein by high-performance liquid chromatography with fluorescence detection using an isocratic solvent system. Analytical Biochemistry 258 (1998) 168-175
21. Mochizuki Y., Suzuki K.T., Sunaga H., Kobayashi T., and Doi R. Separation and characterization of metallothionein in two species of seals by high-performance liquid chromatography-atomic absorption spectrometry. Comparative Biochemistry and Physiology C 82 (1985) 249-254
22. Morgan A.J., Stürzenbaum S.R., Winters C., Grime G.W., Aziz N.A.A., and Kille P. Differential metallothionein expression in earthworm (Lumbricus rubellus) tissues. Ecotoxicology and Environmental Safety 57 (2004) 11-19
23. Nöstelbacher K., Kirchgessner M., and Stangl G.I. Separation and quantitation of metallothionein isoforms from liver of untreated rats by ion-exchange high-performance liquid chromatography and atomic absorption spectrometry. Journal of Chromatography B 744 (2000) 273-282
24. 2+. Analysis by metal summation, SH determination and spectrofluorimetry. Comparative Biochemistry and Physiology Part C 126 (2000) 113-122
25. Prange A., and Schaumlöffel D. Hyphenated techniques for the characterization and quantification of metallothionein isoforms. Analytical and of Bioanalytical Chemistry 373 (2002) 441-453
26. Scott-Fordsmand J.J., and Weeks J.M. Biomarkers in earthworms. Reviews of Environmental Contamination and Toxicology 165 (2000) 117-159
27. Shaw-Allen P., Elliott M., and Jagoe C.H. A microscaled mercury saturation assay for metallothionein in fish. Environmental Toxicology and Chemistry 22 (2003) 2005-2012
28. Stürzenbaum S.R., Kille P., and Morgan A.J. The identification, cloning and characterization of earthworm metallothionein. FEBS Letters 431 (1998) 437-442
29. Stürzenbaum S.R., Winters C., Galay M., Morgan A.J., and Kille P. Metal ion trafficking in earthworms: identification of a cadmium-specific metallothionein. Journal of Biological Chemistry 276 (2001) 34013-34018
30. Stürzenbaum S.R., Georgiev O., Morgan A.J., and Kille P. Cadmium detoxification in earthworms: from genes to cells. Environmental Science & Technology 38 (2004) 6283-6289
31. Svendsen C., Paton G., and Weeks J.M. Soil biomarkers (invertebrates and microbes) for assessing site toxicity. In: Sunahara G.I., Renoux A.Y., Thellen C., Gaudet C.L., and Pilon A. (Eds). Environmental Analysis of Contaminated Sites (2002), Wiley, Chichester, UK 96-133
32. Suzuki K.T. Detection of metallothioneins by high-performance liquid chromatography-inductively coupled plasma emission spectrometry. Methods in Enzymology 205 (1991) 198-205
33. Tang D., Wen L.-S., and Santschi P.H. Analysis of biogenic thiols in natural water sample by high-performance liquid chromatographic separation and fluorescence detection with ammonium 7-fluorobenzo-2-oxa-1,3-diazole-4-sulfonate (SBD-F). Analytica Chimica Acta 408 (2000) 299-307
34. Van Cleef-Toedt K.A., Kaplan L.A.E., and Crivello J.F. Metallothionein mRNA expression in spawning and non-spawning Fundulus heteroclitus following acute exposure to starvation and waterborne cadmium. Fish Physiology and Biochemistry 22 (2000) 319-327
35. Wessel D., and Flügge U.I. A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids. Analytical Biochemistry 138 (1984) 141-143
36. Wong C., Sridhara S., Bardwell J.C.A., and Jakob U. Heating greatly speeds Coomassie blue staining and destaining. BioTechniques 28 (2000) 426-432
37. Yang Y., Maret W., and Vallee B.L. Differential fluorescence labeling of cysteinyl clusters uncovers high tissue levels of thionein. Proceedings of the National Academy of Sciences of the United States of America 98 (2001) 5556-5559