Apoptotic and proliferating hepatocytes differ in prothymosin α expression and cell localization

Molecular and Cellular Biochemistry

Volumn 291, Issue 1-2, 2006, Pages 83-91

  Barbini, Luciana ^a   Gonzalez, Rosario ^a   Dominguez, Fernando ^b   Vega, Felix ^a  

^a Facultad de Veterinaria   (Spain)

^b UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

Author keywords

Apoptosis; Hepatocytes; Liver; Proliferation; Prothymosin

Indexed keywords

PROTHYMOSIN ALPHA;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CYCLE PHASE; CELL CYCLE PROGRESSION; CELL NUCLEUS; CELL PROLIFERATION; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOPLASM; LIVER CELL; LIVER CELL CULTURE; LIVER DISEASE; MALE; NONHUMAN; PATHOGENESIS; PLEIOTROPY; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; RAT;

ANIMALS; APOPTOSIS; CELL PROLIFERATION; CELLS, CULTURED; CYCLOHEXIMIDE; DNA FRAGMENTATION; GENE EXPRESSION REGULATION; HEPATOCYTE GROWTH FACTOR; HEPATOCYTES; MALE; PROTEIN PRECURSORS; PROTEIN TRANSPORT; RATS; RATS, SPRAGUE-DAWLEY; RNA, MESSENGER; THYMOSIN; TRANSFORMING GROWTH FACTOR BETA1;

EID: 33750911217     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-006-9200-0     Document Type: Article

References (34)

1. Haritos AA, GIodall GJ, Horecker BL: Prothymosin alpha: isolation and properties of the major immunoreactive form of thymosin alpha 1 in rat thymus. Proc Natl Acad Sci 81: 1008-1011, 1984
2. Roson E, Garcia-Caballero T, Heimer EP, Felix AM, Dominguez F: Cellular distribution of prothymosin a and parathymosin in rat thymus and spleen. J Histochem Cytochem 38: 1889-1894, 1990
3. Roson E, Gallego R, Garcia-Caballero T, Heimer EP, Felix AM, Dominguez F: Prothymosin alpha expression is associated to cell division in rat testis. Histochemistry 94: 597-599, 1990
4. Gallego R, Roson E, Garcia-Caballero T, Fraga M, Forteza J, Dominguez F, Beiras A: Prothymosin alpha expression in lymph nodes and tonsils. An optical and ultrastructural study. Acta Anat 143: 219-222, 1992
5. Zalvide J, Cancio E, Alvarez CV, Regueiro BJ, Dominguez F: Prothymosin alpha mRNA levels are invariant throughout the cell cycle. J Biol Chem 267: 8692-8695, 1992
6. Alvarez CV, Zalvide JB, Cancio E, Regueiro BJ, Vega FV, Dominguez F: Regulation of prothymosin alpha mRNA levels in rat pituitary tumor cells. Neuroendocrinology 57: 1048-1056, 1993
7. Alvarez CV, Zalvide JB, Cancio E, Dieguez C, Regueiro BJ, Vega FV, Dominguez F: Prothymosin alpha mRNA is expressed in competent and proliferating rat thyroid cells (FRTL-5) but is not sufficient to elicit cell progression throughout the cell cycle. J Mol Endocrinol 11: 249-256, 1993
8. Sburlati AR, Manrow RE, Berger SL: Prothymosin alpha antisense oligomers inhibit myeloma cell division. Proc Natl Acad Sci 88: 253-257, 1991
9. Wu CL, Shiau AL, Lin CS: Prothymosin alpha promotes cell proliferation in NIH3T3 cells. Life Sci 61: 2091-2101, 1997
10. Rodriguez P, Vinuela JE, Alvarez-Fernandez L, Buceta M, Vidal A, Dominguez F, Gomez-Marquez J: Overexpression of prothymosin alpha accelerates proliferation and retards differentiation in HL-60 cells. Biochem J 331: 753-761, 1998
11. Evstafieva AG, Belov GA, Kalkum M, Chichkova NV, Bogdanov AA, Agol VI, Vartapetian AB: Prothymosin alpha fragmentation in apoptosis. FEBS Lett 467: 150-154, 2000
12. Enkemann SA, Wang RH, Trumbore MW, Berger SL: Functional discontinuities in prothymosin alpha caused by caspase cleavage in apoptotic cells. J Cell Physiol 182: 256-268, 2000
13. Kitamura T, Watanabe S, Sato NJ: Gastroenterol Hepatol Supl 1: S96-99, 1998
14. Benedetti A, Marcucci L: The significance of apoptosis in the liver. Liver 19: 453-463, 1999
15. Kanzler S, Galle P: Apoptosis in the liver. Semin Cancer Biol 10: 173-184, 2000
16. Rust C, Gores G: Apoptosis in liver disease. Am J Med 108: 567-574, 2000
17. Schuchmann M, Galle P: Apoptosis in liver disease. European J Gastroenterol Hepatol 13: 785-790, 2001
18. Fraga M, Garcia-Caballero T, Dominguez F, Perez-Becerra E, Beiras A, Forteza J: Immunohistochemical location of prothymosin alpha in regenerating human hepatocytes and hepatocellular carcinoma. Virchows Archiv A Pathol Anat Histopathol 423: 449-452, 1993
19. Wu CG, Boers W, Reitsma PR, van Deventer SJ, Chamuleau RA: Overexpression of prothymosin alpha, concomitant with c-myc, during rat hepatic carcinogenesis. Biochem Biophys Res Commun 32: 817-821, 1997
20. Wu CG, Habib NA, Mitry RR, Reitsma PH, van Deventer SJ, Chamuleau RA: Overexpression of hepatic prothymosin alpha, a novel marker for human hepatocellular carcinoma. Br J Cancer 76: 1199-1204, 1997
21. Pazo JA, Rodriguez ME, Vega F, De la Cruz L, Guibert E, Mediavilla MG, Furno G, Rodriguez JV: Primary Culture of Rat Hepatocytes After Cold Storage in the University of Wisconsin Solution: A Tool to Study the Effects of Hypothermic Preservation. Cell Preservation Technology 1: 189-199, 2002
22. Carneiro C, Alvarez CV, Zalvide J, Vidal A, Dominguez F: TGF-β1 actions on FRTL-5 cells provide a model for the physiological regulation of thyroid growth. Oncogene 16: 1455-1465, 1998
23. Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159, 1987
24. Smith MR, al-Katib A, Mohammad R, Silverman A, Szabo P, Khilnani S, Kohler W, Nath R, Mutchnick MG: Prothymosin alpha gene expression correlates with proliferation, not differentiation, of HL-60 cells. Blood 82: 1127-1132, 1993
25. Castro JM, Barcia MG: Localization of prothymosin alpha in the nucleus. Biochem Biophys Res Commun 224: 140-146, 1996
26. Vareli K, Tsolas O, Frangou-Lazaridis M: Regulation of prothymosin alpha during the cell cycle. Eur J Biochem 238: 799-806, 1996
27. Vareli K, Frangou-Lazaridis M, van der Kraan I, Tsolas O, van Driel R: Nuclear distribution of prothymosin α and parathymosin: Evidence that prothymosin is associated with RNA synthesis processing and parathymosin with DNA replication. Exp Cell Res 257: 152-161, 2000
28. Conteas CN, Mutchnick MG, Palmer KC, Weller FE, Luk GD, Naylor PH, Erdos MR, Goldstein AL, Panneerselvam C, Horecker BL: Cellular levels of thymosin immunoreactive peptides are linked to proliferative events: evidence for a nuclear site of action. Proc Natl Acad Sci 87: 3269-3273, 1990
29. Manrow RE, Sburlati AR, Hanover JA, Berger SL: Nuclear targeting of prothymosin alpha. J Biol Chem 266: 3916-3924, 1991
30. Clinton M, Graeve L, El-Dorry H, Rodriguez-Boulan E, Horecker BL: Evidence for nuclear targeting of prothymosin and parathymosin synthesized in situ. Proc Natl Acad Sci 88: 6608-6612, 1991
31. Gomez-Marquez J, Segade F: Prothymosin alpha is a nuclear protein. FEBS Lett 226: 217-219, 1988
32. Vareli K, Frangou-Lazaridis M: Prothymosin α is localized in mitotic spindle during mitosis. Biol Cell 96: 421-428, 2004
33. Evstafieva AG, Belov GA, Rubtsov YP, Kalkum M, Joshep B, Chichkova NV, Sukhacheva EA, Bogdanov AA, Petterson RF, Agol VI, Vartapetian AB: Apoptosis-related fragmentation, translocation, and properties of human prothymosin alpha. Experim Cell Res 284: 211-223, 2003
34. Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC, Rosenberg S, Wang X: Distinctive roles of PHAP proteins and prothymosin-alpha in a death regulatory pathway. Science 29: 223-226, 2003