Construction of bioactive chimeric MHC class I tetramer by expression and purification of human-murine chimeric MHC heavy chain and β2m as a fusion protein in Escherichia coli

Protein Expression and Purification

Volumn 50, Issue 2, 2006, Pages 171-178

  Ren, Ding ^a   Wang, Fang ^a   He, Xiaowen ^a   Jiang, Lei ^a   Li, Dean ^a   Ying, He ^a   Sun, Shuhan ^a  

^a SECOND MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Biological activity; Chimeric MHC class I complexes; Expression; Fusion protein; Human murine chimeric MHC heavy chain and 2m; pET17b vector; Purification

Indexed keywords

BETA 2 MICROGLOBULIN; CD8 ANTIGEN; HLA A ANTIGEN; HLA A*0201 ANTIGEN; HLA A2 ANTIGEN; HLA ANTIGEN CLASS 1; HLA-A*0201 ANTIGEN; HYBRID PROTEIN;

ANIMAL; ARTICLE; BIOTINYLATION; CYTOTOXIC T LYMPHOCYTE; ESCHERICHIA COLI; FEMALE; GENETICS; HUMAN; IMMUNOLOGY; IN VITRO STUDY; ISOLATION AND PURIFICATION; MOLECULAR CLONING; MOUSE; PLASMID; PROTEIN FOLDING; TRANSGENIC MOUSE;

ANIMALS; ANTIGENS, CD8; BETA 2-MICROGLOBULIN; BIOTINYLATION; CLONING, MOLECULAR; ESCHERICHIA COLI; FEMALE; HISTOCOMPATIBILITY ANTIGENS CLASS I; HLA-A ANTIGENS; HLA-A2 ANTIGEN; HUMANS; MICE; MICE, TRANSGENIC; PLASMIDS; PROTEIN FOLDING; RECOMBINANT FUSION PROTEINS; T-LYMPHOCYTES, CYTOTOXIC;

ESCHERICHIA COLI; MURINAE; MUS MUSCULUS;

EID: 33750831014     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.08.001     Document Type: Article

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