Cloning, expression, and purification of HLA-A2-BSP and β-2m in Escherichia coli

Protein Expression and Purification

Volumn 35, Issue 2, 2004, Pages 210-217

  Piao, Wen Hua ^a,b   Song, Xiao Guo ^c   Liu, Mao Chang ^a   He, Yu ^a   Zhang, Heng Hui ^a   Xu, Wen Xie ^b   Li, Zai Liu ^b   Zhang, He Qiu ^c   Ling, Shi Gan ^c   Wang, Gui Qiang ^a  

^a PEKING UNIVERSITY FIRST HOSPITAL   (China)

^b YANBIAN UNIVERSITY   (China)

^c BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

Author keywords

Expression; HLA A0201 BSP and 2m genes; pBV220 vector; Purification

Indexed keywords

ESCHERICHIA COLI; PROKARYOTA;

EID: 2642546656     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.01.002     Document Type: Article

References (27)

1. K.C. Parker, B.M. Carreno, L. Sestak, U. Utz, W.E. Biddison, J.E. Coligan, Peptide binding to HLA-A2 and HLA-B27 isolated from Escherichia coli. Reconstitution of HLA-A2 and HLA-B27 heavy chain/beta 2-microglobulin complexes requires specific peptides, J. Biol. Chem. 267 (1992) 5451-5459.
2. Y. Sato, H. Sahara, T. Tsukahara, M. Kondo, Y. Hirohashi, Y. Nabeta, S. Kawguchi, H. Ikeda, T. Toreigoe, S. Ichiiya, Y. Tamura, T. Wada, T. Yamashita, M. Goto, H. Takasuc, N. Sato, Improved generation of HLA class I/peptide tetramers, J. Immunol. Methods, 271 (2002) 177-184.
3. G.S. Ogg, A.J. McMichael, Quantitation of antigen-specific CD8+ T-cell responses, Immunol. Lett. 66 (1999) 77-80.
4. N. Anikeeva, T. Lebedeva, M. Sumaroka, S.A. Kalms, Y. Sykulev, Soluble HIV-specific T cell receptor: expression, purification and analysis of the specificity, J. Immunol. Methods 277 (2003) 75-86.
5. V. Cerundolo, Use of major histocompatibility complex class I tetramers to, monitor tumor-specific cytotoxic T lymphocyte response in melanoma patients, Cancer Chemother. Pharmacol. 46 (2000) S83-S85.
6. S.J. Youde, P.R. Dunbar, E.M. Evans, A.N. Fiander, L.K. Borysiewicz, V. Cerundolo, S. Man, Use of fluorogenic histocompatibility leukocyte antigen-A 0201/HPV 16 E7 peptide complexes to isolate rare human cytotoxic T-lymphocyte recognizing endogenous human papillomavirus antigens, Cancer Res. 60 (2000) 365-371.
7. J. Sambrook, E.F. Fritsch, T. Maniatis, Molecular Cloning: A Laboratory Manual [M], second ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
8. G.G.B. Klaus, Lymphocytes [M]. Information Printing td., Oxford, 1987, 163-167.
9. C. Taswell, Limiting dilution assays for the determination of immunocompetent cell frequencies I. Data analysis, J. Immunol. 126 (1981) 1614-1619.
10. K. Kuzushima, Y. Hoshino, K. Fujii, N. Yokoyama, M. Fujita, T. Kiyono, H. Kimura, T. Morishima, Y. Morishima, T. Tsurumi, Rapid determination of Epstein-Barr virus-specific CD8(+) T-cell frequencies by flow cytometry, Blood 94 (1999) 3094-3100.
11. L.C. Tan, N. Gudgeon, N.E. Annels, P. Hansasuta, C.A. O'Callaghan, S. Rowland-Jones, A.J. McMichael, A.B. Rickinson, M.F. Callan, A re-evaluation of the frequency of CD8+ T cells specific for EBV in healthy virus carriers, J. Immunol. 62 (1999) 1827-1835.
12. C. Scheibenbogen, K.H. Lee, S. Mayer, S. Stevanovic, U. Moebius, W. Herr, H.G. Rammensee, U. Keilholz, A sensitive ELISPOT assay for detection of CD8+ T lymphocytes specific for HLA class I-binding peptide epitopes derived from influenza proteins in the blood of healthy donors and melanoma patients, Clin. Cancer Res. 3 (2) (1997) 221-226.
13. T.M. Clay, A.C. Hobeika, P.J. Mosca, H.K. Lyerly, M.A. Morse, Assays for monitoring cellular immune responses to active immunotherapy of cancer, Clin. Cancer Res. 7 (2001) 1127-1135.
14. C. Czerkinsky, G. Andersson, H.P. Ekre, L.A. Nilsson, L. Klareskog, O. Ouchterlony, Reverse ELISPOT assay for clonal analysis of cytokine production. I. Enumeration of gamma-interferon-secreting cells, J. Immunol. Methods 110 (1) (1988) 29-36.
15. E. Jager, Y. Nagata, S. Gnjatic, H. Wada, E. Stockert, J. Karbach, P.R. Dunbar, S.Y. Lee, A. Jungbluth, D. Jager, M. Arand, G. Ritter, V. Cerundolo, B. Dupont, Y.T. Chen, L.J. Old, A. Knuth, Monitoring CD8 T cell responses to NY-ESO-1: correlation of humoral and cellular immune responses, Proc. Natl. Acad. Sci. USA 97 (2000) 4760-4765.
16. C. Scheibenbogen, P. Romero, L. Rivoltini, W. Herr, A. Schmittel, J.C. Cerottini, T. Woelfel, A.M. Eggermont, U. Keilholz, Quantitation of antigen-reactive T cells in peripheral blood by IFNgamma-ELISPOT assay and chromium-release assay: a four-centre comparative trial, J. Immunol. Methods 244 (2000) 81-89.
17. T. Jung, Detecion of intracellular cytokines by flow cytometry, J. Immunol. Methods 519 (1993) 197-207.
18. V.C. Maino, L.J. Picker, Identification of functional subsets by flow cytometry: intracellular detection of cytokine expression, Cytometry 34 (1998) 207-215.
19. V. Appay, S.L. Rowland-Jones, The assessment of antigen-specific CD8+ T cells through the combination of MHC class I tetramer and intracellular staining, J. Immunol. Methods 268 (2002) 9-19.
20. P. Romero, P.R. Dunbar, D. Valmori, M. Pittet, G.S. Ogg, D. Rimoldi, J.L. Chen, D. Lienard, J.C. Cerottini, V. Cerundolo, Ex vivo staining of metastatic lymph nodes by class I major histocompatibility complex tetramers reveals high numbers of antigen-experienced tumor-specific cytolytic T lymphocytes, J. Exp. Med. 188 (1998) 1641-1650.
21. P.P. Lee, C. Yee, P.A. Savage, L. Fong, D. Brockstedt, J.S. Weber, D. Johnson, S. Swetter, J. Thompson, P.D. Greenberg, M. Roederer, M.M. Davis, Characterization of circulating T cells specific for tumor-associated antigens in melanoma patients, Nat. Med. Nat. Med. 5 (1999) 677-685.
22. D. Valmori, V. Dutoit, D. Lienard, F. Lejeune, D. Speiser, D. Rimoldi, V. Cerundolo, P.Y. Dietrich, J.C. Cerottini, P. Romero, Tetramer-guided analysis of TCR beta-chain usage reveals a large repertoire of melan-A-specific CD8+ T cells in melanoma patients, J. Immunol. 165 (2000) 533-538.
23. B. Maier, H.J. Buhring, M. Simon, K. Eichmann, I. Melchers, Limiting dilution analysis of proliferating and helper T cells in the in vivo immune response to KLH: derepression of helper T cells at moderately increased frequencies, J. Mol. Cell Immunol. 2 (1986) 293-305.
24. J.A. Owen, M. Allouche, P.C. Doherty, Limiting dilution analysis of the specificity of influenza-immune cytotoxic T cells, Cell Immunol. 67 (1982) 49-59.
25. K. Murali-Krishna, J.D. Altman, M. Suresh, D.J. Sourdive, A.J. Zajac, J.D. Miller, J. Slansky, R. Ahmed, Counting antigen-specific CD8 T cells: a reevaluation of bystander activation during viral infection, Immunity 8 (1998) 177-187.
26. J.D. Altman, P.A. Moss, P.J. Goulder, D.H. Barouch, M.G. McHeyzer-Williams, J.I. Bell, A.J. McMichael, M.M. Davis, Phenotypic analysis of antigen-specific T lymphocytes, Science 274 (5284) (1996) 94-96, Erratum in: Science 1998 June 19; 280 (5371): 1821.
27. Z.-q. Zhang, L.-h. Yao, Y.-d. Hou, Construction and application of a high level expression vector containing PRPL promoter, Chin. J. Virol. 6 (1990) 111-116.