Secretory expression of synthetic human Fas ligand extracellular domain gene in Pichia pastoris: Influences of tag addition and N-glycosylation site deletion, and development of a purification method

Protein Expression and Purification

Volumn 50, Issue 2, 2006, Pages 137-146

  Muraki, Michiro ^a  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

Author keywords

Extracellular domain; Human Fas ligand; N Glycosylation site deletion; Pichia pastoris; Purification; Secretory expression; Tag addition

Indexed keywords

FAS LIGAND; HYBRID PROTEIN;

AMINO ACID SEQUENCE; ARTICLE; GENETICS; GLYCOSYLATION; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PICHIA; PROTEIN TERTIARY STRUCTURE; SECRETION;

AMINO ACID SEQUENCE; CLONING, MOLECULAR; FAS LIGAND PROTEIN; GLYCOSYLATION; HUMANS; MOLECULAR SEQUENCE DATA; PICHIA; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT FUSION PROTEINS;

PICHIA PASTORIS;

EID: 33750813509     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.08.006     Document Type: Article

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