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33750614085
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note
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Rpn denotes the ratio of the positive maximum intensity over the negative maximum intensity and is used as index of triple-helical fold and stability (see also ref. [12]).
-
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-
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37
-
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33750622397
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note
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-1) at 4°C for peptide 2 (1.09) and peptide 3 (0.90 before and 0.82, after irradiation) are indicative of trimeric species; at 27°C the values for peptide 2 (1.34) and for peptide 3 before irradiation (1.15) correspond again to trimeric species, while after irradiation of 3 in addition to the trimeric species (0.61 and 1.07) a second species is formed, which shows a diffusion constant of 1.55 supporting the presence of an unfolded monomeric form.
-
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38
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33750608966
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-
note
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1H NMR spectra at δ = 9.34 and 9.08 ppm (4°C), 9.21 and 8.94 ppm (22°C), and 9.18 and 8.86 ppm (27°C), respectively.
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