Rhodopsin-transducin coupling: Role of the Gα C-terminus in nucleotide exchange catalysis

Vision Research

Volumn 46, Issue 27, 2006, Pages 4582-4593

  Herrmann, Rolf ^a   Heck, Martin ^a   Henklein, Peter ^a   Kleuss, Christiane ^a   Wray, Victor ^b   Hofmann, Klaus Peter ^a   Ernst, Oliver P ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

Author keywords

C terminal mutations; Nucleotide exchange catalysis; Rhodopsin; Signal transduction; Transducin

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT; GUANINE NUCLEOTIDE BINDING PROTEIN BETA SUBUNIT; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; RHODOPSIN; TRANSDUCIN;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMAL TISSUE; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CATALYST; CONTROLLED STUDY; MOLECULAR DYNAMICS; MOLECULAR INTERACTION; NONHUMAN; NUCLEOTIDE EXCHANGE CATALYSIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN INTERACTION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION;

AMINO ACID SUBSTITUTION; ANIMALS; CATTLE; GTP-BINDING PROTEIN ALPHA SUBUNITS; HYDROPHOBICITY; MAGNETIC RESONANCE SPECTROSCOPY; PHOTOTRANSDUCTION; PROTEIN BINDING; PROTEIN CONFORMATION; RHODOPSIN; ROD OUTER SEGMENTS; TERMINAL REPEAT SEQUENCES; TRANSDUCIN;

EID: 33750615086     PISSN: 00426989     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.visres.2006.07.027     Document Type: Article

References (38)

1. Acharya S., Saad Y., and Karnik S.S. Transducin-alpha C-terminal peptide binding site consists of C-D and E-F loops of rhodopsin. Journal of Biological Chemistry 272 (1997) 6519-6524
2. Arimoto R., Kisselev O.G., Makara G.M., and Marshall G.R. Rhodopsin-transducin interface: studies with conformationally constrained peptides. Biophysical Journal 81 (2001) 3285-3293
3. Aris L., Gilchrist A., Rens-Domiano S., Meyer C., Schatz P.J., Dratz E.A., et al. Structural requirements for the stabilization of metarhodopsin II by the C terminus of the alpha subunit of transducin. Journal of Biological Chemistry 276 (2001) 2333-2339
4. Bartl F., Ritter E., and Hofmann K.P. FTIR spectroscopy of complexes formed between metarhodopsin II and C-terminal peptides from the G-protein alpha- and gamma-subunits. FEBS Letters 473 (2000) 259-264
5. Brabazon D.M., Abdulaev N.G., Marino J.P., and Ridge K.D. Evidence for structural changes in carboxyl-terminal peptides of transducin alpha-subunit upon binding a soluble mimic of light-activated rhodopsin. Biochemistry 42 (2003) 302-311
6. Cabrera-Vera T.M., Vanhauwe J., Thomas T.O., Medkova M., Preininger A., Mazzoni M.R., et al. Insights into G protein structure, function, and regulation. Endocrine Reviews 24 (2003) 765-781
7. Cai K., Itoh Y., and Khorana H.G. Mapping of contact sites in complex formation between transducin and light-activated rhodopsin by covalent crosslinking: use of a photoactivatable reagent. Proceedings of the National Academy of Sciences of the United States of America 98 (2001) 4877-4882
8. Cai K., Langen R., Hubbell W.L., and Khorana H.G. Structure and function in rhodopsin: topology of the C-terminal polypeptide chain in relation to the cytoplasmic loops. Proceedings of the National Academy of Sciences of the United States of America 94 (1997) 14267-14272
9. Chabre M., and le Maire M. Monomeric G-protein-coupled receptor as a functional unit. Biochemistry 44 (2005) 9395-9403
10. Emeis D., Kühn H., Reichert J., and Hofmann K.P. Complex formation between metarhodopsin II and GTP-binding protein in bovine photoreceptor membranes leads to a shift of the photoproduct equilibrium. FEBS Letters 143 (1982) 29-34
11. Ernst O.P., and Bartl F.J. Active States of Rhodopsin. Chembiochem 3 (2002) 968-974
12. Ernst O.P., Bieri C., Vogel H., and Hofmann K.P. Intrinsic biophysical monitors of transducin activation: fluorescence, UV-visible spectroscopy, light scattering, and evanescent field techniques. Methods in Enzymology 315 (2000) 471-489
13. Ernst O.P., Meyer C.K., Marin E.P., Henklein P., Fu W.Y., Sakmar T.P., et al. Mutation of the fourth cytoplasmic loop of rhodopsin affects binding of transducin and peptides derived from the carboxyl-terminal sequences of transducin alpha and gamma subunits. Journal of Biological Chemistry 275 (2000) 1937-1943
14. Garcia P.D., Onrust R., Bell S.M., Sakmar T.P., and Bourne H.R. Transducin-alpha C-terminal mutations prevent activation by rhodopsin: a new assay using recombinant proteins expressed in cultured cells. EMBO Journal 14 (1995) 4460-4469
15. Hamm H.E., Deretic D., Arendt A., Hargrave P.A., König B., and Hofmann K.P. Site of G protein binding to rhodopsin mapped with synthetic peptides from the alpha subunit. Science 241 (1988) 832-835
16. Heck M., and Hofmann K.P. G-protein-effector coupling: a real-time light-scattering assay for transducin-phosphodiesterase interaction. Biochemistry 32 (1993) 8220-8227
17. Heck M., and Hofmann K.P. Maximal rate and nucleotide dependence of rhodopsin-catalyzed transducin activation: initial rate analysis based on a double displacement mechanism. Journal of Biological Chemistry 276 (2001) 10000-10009
18. Heck M., Pulvermüller A., and Hofmann K.P. Light scattering methods to monitor interactions between rhodopsin-containing membranes and soluble proteins. Methods in Enzymology 315 (2000) 329-347
19. Herrmann R., Heck M., Henklein P., Henklein P., Kleuss C., Hofmann K.P., et al. Sequence of interactions in receptor-G protein coupling. Journal of Biological Chemistry 279 (2004) 24283-24290
20. Herrmann, R., Heck, M., Henklein, P., Hofmann K. P., & Ernst, O. P. (2006). Signal transfer from GPCRs to G proteins: role of the Galpha N-terminal region in rhodopsin-transducin coupling. Journal of Biological Chemistry, in press.
21. Janz J.M., and Farrens D.L. Rhodopsin activation exposes a key hydrophobic binding site for the transducin alpha-subunit C terminus. Journal of Biological Chemistry 279 (2004) 29767-29773
22. Kisselev O.G., and Downs M.A. Rhodopsin controls a conformational switch on the transducin gamma subunit. Structure 11 (2003) 367-373
23. Kisselev O.G., Ermolaeva M.V., and Gautam N. A farnesylated domain in the G protein gamma subunit is a specific determinant of receptor coupling. Journal of Biological Chemistry 269 (1994) 21399-21402
24. Kisselev O.G., Kao J., Ponder J.W., Fann Y.C., Gautam N., and Marshall G.R. Light-activated rhodopsin induces structural binding motif in G protein alpha subunit. Proceedings of the National Academy of Sciences of the United States of America 95 (1998) 4270-4275
25. Kisselev O.G., Meyer C.K., Heck M., Ernst O.P., and Hofmann K.P. Signal transfer from rhodopsin to the G-protein: evidence for a two-site sequential fit mechanism. Proceedings of the National Academy of Sciences of the United States of America 96 (1999) 4898-4903
26. Klein-Seetharaman J., Hwa J., Cai K., Altenbach C., Hubbell W.L., and Khorana H.G. Single-cysteine substitution mutants at amino acid positions 55-75, the sequence connecting the cytoplasmic ends of helices I and II in rhodopsin: reactivity of the sulfhydryl groups and their derivatives identifies a tertiary structure that changes upon light-activation. Biochemistry 38 (1999) 7938-7944
27. Koenig B.W., Kontaxis G., Mitchell D.C., Louis J.M., Litman B.J., and Bax A. Structure and orientation of a G protein fragment in the receptor bound state from residual dipolar couplings. Journal of Molecular Biology 322 (2002) 441-461
28. Lambright D.G., Sondek J., Bohm A., Skiba N.P., Hamm H.E., and Sigler P.B. The 2.0 Å crystal structure of a heterotrimeric G protein. Nature 379 (1996) 311-319
29. Martin E.L., Rens-Domiano S., Schatz P.J., and Hamm H.E. Potent peptide analogues of a G protein receptor-binding region obtained with a combinatorial library. Journal of Biological Chemistry 271 (1996) 361-366
30. Morizumi T., Imai H., and Shichida Y. Two-step mechanism of interaction of rhodopsin intermediates with the c-terminal region of the transducin alpha-subunit. Journal of Biochemistry 134 (2003) 259-267
31. Natochin M., Muradov K.G., McEntaffer R.L., and Artemyev N.O. Rhodopsin recognition by mutant G(s)alpha containing C-terminal residues of transducin. Journal of Biological Chemistry 275 (2000) 2669-2675
32. Okada T., Ernst O.P., Palczewski K., and Hofmann K.P. Activation of rhodopsin: new insights from structural and biochemical studies. Trends in Biochemical Sciences 26 (2001) 318-324
33. Onrust R., Herzmark P., Chi P., Garcia P.D., Lichtarge O., Kingsley C., et al. Receptor and betagamma binding sites in the alpha subunit of the retinal G protein transducin. Science 275 (1997) 381-384
34. Park P.S., Filipek S., Wells J.W., and Palczewski K. Oligomerization of G protein-coupled receptors: past, present, and future. Biochemistry 43 (2004) 15643-15656
35. Parkes J.H., and Liebman P.A. Temperature and pH dependence of the metarhodopsin I-metarhodopsin II kinetics and equilibria in bovine rod disk membrane suspensions. Biochemistry 23 (1984) 5054-5061
36. Terakita A., Yamashita T., Nimbari N., Kojima D., and Shichida Y. Functional interaction between bovine rhodopsin and G protein transducin. Journal of Biological Chemistry 277 (2002) 40-46
37. Wall M.A., Coleman D.E., Lee E., Iniguez-Lluhi J.A., Posner B.A., Gilman A.G., et al. The structure of the G protein heterotrimer Gi alpha 1 beta 1 gamma 2. Cell 83 (1995) 1047-1058
38. Wang X., Kim S.H., Ablonczy Z., Crouch R.K., and Knapp D.R. Probing rhodopsin-transducin interactions by surface modification and mass spectrometry. Biochemistry 43 (2004) 11153-11162