Ezrin mediates growth and survival in Ewing's sarcoma through the AKT/mTOR, but not the MAPK, signaling pathway

Clinical and Experimental Metastasis

Volumn 23, Issue 3-4, 2006, Pages 227-236

  Krishnan, Kartik ^a   Bruce, Ben ^a   Hewitt, Stephen ^a   Thomas, Dafydd ^b   Khanna, Chand ^a   Helman, Lee J ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

AKT; Ewing's sarcoma; Ezrin; Immunohistochemistry

Indexed keywords

EZRIN; MAMMALIAN TARGET OF RAPAMYCIN; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN KINASE B;

ARTICLE; CELL GROWTH; CELL SURVIVAL; CONTROLLED STUDY; EWING SARCOMA; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; IN VIVO CULTURE; MOUSE; NONHUMAN; OSTEOSARCOMA; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; RHABDOMYOSARCOMA; WESTERN BLOTTING;

ANIMALS; APOPTOSIS; CELL PROLIFERATION; CELL SURVIVAL; CYTOSKELETAL PROTEINS; HUMANS; MICE; MITOGEN-ACTIVATED PROTEIN KINASES; MUTATION; NEOPLASM METASTASIS; PROTO-ONCOGENE PROTEINS C-AKT; SARCOMA, EWING'S; SIGNAL TRANSDUCTION; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 33750573063     PISSN: 02620898     EISSN: None     Source Type: Journal    
DOI: 10.1007/s10585-006-9033-y     Document Type: Article

References (35)

1. West DC, Grier HE, Swallow MM et al (1997) Detection of circulating tumor cells in patients with Ewing's sarcoma and peripheral primitive neuroectodermal tumor. J Clin Oncol 15:583-588
2. Wang CC, Schulz MD (1953) Ewing's sarcoma; a study of fifty cases treated at the Massachusetts General Hospital, 1930-1952 inclusive. N Engl J Med 248:571-576
3. Krishnan K, Khanna C, Helman LJ (2005) The biology of metastases in pediatric sarcomas. Cancer J 11:306-313
4. Khanna C, Wan X, Bose S et al (2004) The membrane-cytoskeleton linker ezrin is necessary for osteosarcoma metastasis. Nat Med 10:182-186
5. Yu Y, Khan J, Khanna C et al (2004) Expression profiling identifies the cytoskeletal organizer ezrin and the developmental homeoprotein Six-1 as key metastatic regulators. Nat Med 10:175-181
6. Hiscox S, Jiang WG (1999) Ezrin regulates cell-cell and cell-matrix adhesion, a possible role with E-cadherin/betacatenin. J Cell Sci 112(Pt 18):3081-3090
7. Luciani F, Molinari A, Lozupone F et al (2002) P-glycoprotein-actin association through ERM family proteins: a role in P-glycoprotein function in human cells of lymphoid origin. Blood 99:641-648
8. Parlato S, Giammarioli AM, Logozzi M et al (2000) CD95 (APO-1/Fas) linkage to the actin cytoskeleton through ezrin in human T lymphocytes: a novel regulatory mechanism of the CD95 apoptotic pathway. EMBO J 19:5123-5134
9. Perez OD, Kinoshita S, Hitoshi Y et al (2002) Activation of the PKB/AKT pathway by ICAM-2. Immunity 16:51-65
10. Gautreau A, Poullet P, Louvard D, Arpin M (1999) Ezrin, a plasma membrane-microfilament linker, signals cell survival through the phosphatidylinositol 3-kinase/Akt pathway. Proc Natl Acad Sci USA 96:7300-7305
11. Nicosia SV, Bai W, Cheng JQ et al (2003) Oncogenic pathways implicated in ovarian epithelial cancer. Hematol Oncol Clin North Am 17:927-943
12. Shi-Wen X, Chen Y, Denton CP et al (2004) Endothelin-1 promotes myofibroblast induction through the ETA receptor via a rac/phosphoinositide 3-kinase/Akt-dependent pathway and is essential for the enhanced contractile phenotype of fibrotic fibroblasts. Mol Biol Cell 15:2707-2719
13. Wick W, Grimmel C, Wild-Bode C et al (2001) Ezrin-dependent promotion of glioma cell clonogenicity, motility, and invasion mediated by BCL-2 and transforming growth factor-beta2. J Neurosci 21:3360-3368
14. Wu KL, Khan S, Lakhe-Reddy S et al (2004) The NHE1 Na+/H+ exchanger recruits ezrin/radixin/moesin proteins to regulate Akt-dependent cell survival. J Biol Chem 279:26280-26286
15. Elliott BE, Meens JA, SenGupta SK et al (2005) The membrane cytoskeletal crosslinker ezrin is required for metastasis of breast carcinoma cells. Breast Cancer Res 7:R365-R373
16. Nakamura F, Amieva MR, Furthmayr H (1995) Phosphorylation of threonine 558 in the carboxyl-terminal actin-binding domain of moesin by thrombin activation of human platelets. J Biol Chem 270:31377-31385
17. Matsui T, Maeda M, Doi Y et al (1998) Rho-kinase phosphorylates COOH-terminal threonines of ezrin/radixin/moesin (ERM) proteins and regulates their head-to-tail association. J Cell Biol 140:647-657
18. Gautreau A, Louvard D, Arpin M (2000) Morphogenic effects of ezrin require a phosphorylation-induced transition from oligomers to monomers at the plasma membrane. J Cell Biol 150:193-203
19. Wan X, Mendoza A, Khanna C, Helman LJ (2005) Rapamycin inhibits ezrin-mediated metastatic behavior in a murine model of osteosarcoma. Cancer Res 65:2406-2411
20. Kontny HU, Lehrnbecher TM, Chanock SJ, Mackall CL (1998) Simultaneous expression of Fas and nonfunctional Fas ligand in Ewing's sarcoma. Cancer Res 58:5842-5849
21. Hewitt SM (2004) Design, construction, and use of tissue microarrays. Methods Mol Biol 264:61-72
22. Hirao M, Sato N, Kondo T et al (1996) Regulation mechanism of ERM (ezrin/radixin/moesin) protein/plasma membrane association: possible involvement of phosphatidylinositol turnover and Rho-dependent signaling pathway. J Cell Biol 135:37-51
23. Simons PC, Pietromonaco SF, Reczek D et al (1998) C-terminal threonine phosphorylation activates ERM proteins to link the cell's cortical lipid bilayer to the cytoskeleton. Biochem Biophys Res Commun 253:561-565
24. Gould KL, Bretscher A, Esch FS, Hunter T (1989) cDNA cloning and sequencing of the protein-tyrosine kinase substrate, ezrin, reveals homology to band 4.1. EMBO J 8:4133-4142
25. Arpin M, Algrain M, Louvard D (1994) Membrane-actin microfilament connections: an increasing diversity of players related to band 4.1. Curr Opin Cell Biol 6:136-141
26. Ilmonen S, Vaheri A, Asko-Seljavaara S, Carpen O (2005) Ezrin in primary cutaneous melanoma. Mod Pathol 18:503-510
27. Weng WH, Ahlen J, Astrom K et al (2005) Prognostic impact of immunohistochemical expression of ezrin in highly malignant soft tissue sarcomas. Clin Cancer Res 11:6198-6204
28. Lugini L, Lozupone F, Matarrese P et al (2003) Potent phagocytic activity discriminates metastatic and primary human malignant melanomas: a key role of ezrin. Lab Invest 83:1555-1567
29. McClatchey AI (2003) Merlin and ERM proteins: unappreciated roles in cancer development? Nat Rev Cancer 3:877-883
30. Benini S, Manara MC, Cerisano V et al (2004) Contribution of MEK/MAPK and PI3-K signaling pathway to the malignant behavior of Ewing's sarcoma cells: therapeutic prospects. Int J Cancer 108:358-366
31. Alessi DR, Andjelkovic M Caudwell B et al (1996) Mechanism of activation of protein kinase B by insulin and IGF-1. EMBO J 15:6541-6551
32. Burgering BM, Coffer PJ (1995) Protein kinase B (c-Akt) in phosphatidylinositol-3-OH kinase signal transduction. Nature 376:599-602
33. Franke TF, Yang SI, Chan TO et al (1995) The protein kinase encoded by the Akt proto-oncogene is a target of the PDGF-activated phosphatidylinositol 3-kinase. Cell 81:727-736
34. Toretsky JA, Thakar M, Eskenazi AE, Frantz CN (1999) Phosphoinositide 3-hydroxide kinase blockade enhances apoptosis in the Ewing's sarcoma family of tumors. Cancer Res 59:5745-5750
35. Bjornsti MA, Houghton PJ (2004) The TOR pathway: a target for cancer therapy. Nat Rev Cancer 4:335-348