Antigenic variation of TprK V regions abrogates specific antibody binding in syphilis

Infection and Immunity

Volumn 74, Issue 11, 2006, Pages 6244-6251

  LaFond, Rebecca E ^a   Molini, Barbara J ^a   Van Voorhis, Wesley C ^a   Lukehart, Sheila A ^a,b  

^a UNIVERSITY OF WASHINGTON   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ANTIGEN; BACTERIUM ANTIBODY; SYNTHETIC PEPTIDE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIBODY RESPONSE; ANTIBODY SPECIFICITY; ANTIGEN BINDING; ANTIGENIC VARIATION; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; CLONE; CROSS REACTION; GENE EXPRESSION; GENE SEQUENCE; GENETIC VARIABILITY; IMMUNE RESPONSE; IMMUNOASSAY; NONHUMAN; PRIORITY JOURNAL; RABBIT; SPIROCHETE; SUBSPECIES; SYPHILIS; TECHNIQUE; TPRK GENE; TREPONEMA PALLIDUM;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODIES, BACTERIAL; ANTIBODY SPECIFICITY; ANTIGENIC VARIATION; BACTERIAL OUTER MEMBRANE PROTEINS; BINDING SITES, ANTIBODY; CHRONIC DISEASE; CLONE CELLS; MOLECULAR SEQUENCE DATA; RABBITS; SYPHILIS; TREPONEMA PALLIDUM;

EID: 33750468688     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.00827-06     Document Type: Article

References (19)

1. Baker-Zander, S. A., and S. A. Lukehart. 1992. Macrophage-mediated killing of opsonized Treponema pallidum. J. Infect. Dis. 165:69-74.
2. Baker-Zander, S. A., J. M. Shaffer, and S. A. Lukehart. 1993. Characterization of the serum requirement for macrophage-mediated killing of Treponema pallidum ssp. pallidum: relationship to the development of opsonizing antibodies. FEMS Immunol. Med. Microbiol. 6:273-279.
3. Centurion-Lara, A., C. Castro, L. Barrett, C. Cameron, M. Mostowfi, W. C. Van Voorhis, and S. A. Lukehart. 1999. Treponema pallidum major sheath protein homologue Tpr K is a target of opsonic antibody and the protective immune response. J. Exp. Med. 189:647-656.
4. Centurion-Lara, A., C. Godornes, C. Castro, W. C. Van Voorhis, and S. A. Lukehart. 2000. The tprK gene is heterogeneous among Treponema pallidum strains and has multiple alleles. Infect. Immun. 68:824-831.
5. Centurion-Lara, A., R. E. LaFond, K. Hevner, C. Godornes, B. J. Molini, W. C. Van Voorhis, and S. A. Lukehart. 2004. Gene conversion: a mechanism for generation of heterogeneity in the tprK gene of Treponema pallidum during infection. Mol. Microbiol. 52:1579-1596.
6. Centurion-Lara, A., E. S. Sun, L. K. Barrett, C. Castro, S. A. Lukehart, and W. C. Van Voorhis. 2000. Multiple alleles of Treponema pallidum repeat gene D in Treponema pallidum isolates. J. Bacteriol. 182:2332-2335.
7. Fan, J., and R. S. Stephens. 1997. Antigen conformation dependence of Chlamydia trachomatis infectivity neutralization. J. Infect. Dis. 176:713-721.
8. LaFond, R. E., A. Centurion-Lara, C. Godornes, A. M. Rompalo, W. C. Van Voorhis, and S. A. Lukehart. 2003. Sequence diversity of Treponema pallidum subsp. pallidum tprK in human syphilis lesions and rabbit-propagated isolates. J. Bacteriol. 185:6262-6268.
9. LaFond, R. E., A. Centurion-Lara, C. Godornes, W. C. Van Voorhis, and S. A. Lukehart. 2006. TprK sequence diversity accumulates during rabbit infection with Treponema pallidum subsp. pallidum Nichols strain. Infect. Immun. 74:1896-1906.
10. Lukehart, S. A., S. A. Baker-Zander, R. M. Lloyd, and S. Sell. 1980. Characterization of lymphocyte responsiveness in early experimental syphilis. II. Nature of cellular infiltration and Treponema pallidum distribution in testicular lesions. J. Immunol. 124:461-467.
11. Lukehart, S. A., and J. N. Miller. 1978. Demonstration of the in vitro phagocytosis of Treponema pallidum by rabbit peritoneal macrophages. J. Immunol. 121:2014-2024.
12. Lukehart, S. A., J. M. Shaffer, and S. A. Baker-Zander. 1992. A subpopulation of Treponema pallidum is resistant to phagocytosis: possible mechanism of persistence. J. Infect. Dis. 166:1449-1453.
13. Morgan, C. A., S. A. Lukehart, and W. C. Van Voorhis. 2003. Protection against syphilis correlates with specificity of antibodies to the variable regions of Treponema pallidum repeat protein K. Infect. Immun. 71:5605-5612.
14. Morgan, C. A., B. J. Molini, S. A. Lukehart, and W. C. Van Voorhis. 2002. Segregation of B and T cell epitopes of Treponema pallidum repeat protein K to variable and conserved regions during experimental syphilis infection. J. Immunol. 169:952-957.
15. Stamm, L. V., and H. L. Bergen. 2000. The sequence-variable, single-copy tprK gene of Treponema pallidum Nichols strain UNC and Street strain 14 encodes heterogeneous TprK proteins. Infect. Immun. 68:6482-6486.
16. Suarez-Alvarez, B., M. Garcia-Suarez Mdel, F. J. Mendez, and J. R. de los Toyos. 2003. Characterisation of mouse monoclonal antibodies for pneumolysin: fine epitope mapping and V gene usage. Immunol. Lett. 88:227-239.
17. Sun, E. S., B. J. Molini, L. K. Barrett, A. Centurion-Lara, S. A. Lukehart, and W. C. Van Voorhis. 2004. Subfamily I Treponema pallidum repeat protein family: sequence variation and immunity. Microbes Infect. 6:725-737.
18. Van Voorhis, W. C., L. K. Barrett, D. M. Koelle, J. M. Nasio, F. A. Plummer, and S. A. Lukehart. 1996. Primary and secondary syphilis lesions contain mRNA for Th1 cytokines. J. Infect. Dis. 173:491-495.
19. Wolf, K., E. Fischer, D. Mead, G. Zhong, R. Peeling, B. Whitmire, and H. D. Caldwell. 2001. Chlamydia pneumoniae major outer membrane protein is a surface-exposed antigen that elicits antibodies primarily directed against conformation-dependent determinants. Infect. Immun. 69:3082-3091.