Effect of CaCl2 as activity stabilizer on purification of heparinase I from Flavobacterium heparinum

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 843, Issue 2, 2006, Pages 209-215

  Ma, Xiaolai ^a   Wang, Zunsheng ^a,b   Li, Suxia ^a   Shen, Qiong ^a   Yuan, Qinsheng ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b SHENYANG NORMAL UNIVERSITY   (China)

Author keywords

Activity stabilizer; CaCl2; Flavobacterial heparinum; Heparinase I; Purification

Indexed keywords

BACTERIA; CELL CULTURE; CHROMATOGRAPHY; PH EFFECTS; PRECIPITATION (CHEMICAL); PURIFICATION;

ACTIVITY STABILIZER; CACL2; FLAVOBACTERIAL HEPARINUM;

ENZYMES;

AMMONIUM SULFATE; BACTERIAL ENZYME; CALCIUM CHLORIDE; HEPARIN LYASE; SEPHADEX; SEPHAROSE;

ARTICLE; ENZYME ACTIVITY; ENZYME PURIFICATION; FLAVOBACTERIUM; FLAVOBACTERIUM HEPARINUM; FREEZE THAWING; GEL FILTRATION CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN PURIFICATION; DRUG EFFECT; ENZYME STABILITY; ENZYMOLOGY; GEL CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METHODOLOGY; TEMPERATURE;

PEDOBACTER HEPARINUS;

CALCIUM CHLORIDE; CHROMATOGRAPHY, GEL; ENZYME STABILITY; FLAVOBACTERIUM; HEPARIN LYASE; HYDROGEN-ION CONCENTRATION; TEMPERATURE;

EID: 33750369353     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2006.06.015     Document Type: Article

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