Purification and characterization of heparinase that degrades both heparin and heparan sulfate from bacillus circulans

Bioscience, Biotechnology and Biochemistry

Volumn 66, Issue 5, 2002, Pages 1181-1184

  Yoshida, Eiichi ^a   Sakai, Kazuya ^a   Tokuyama, Shinji ^a   Miyazono, Hirofumi ^b   Maruyama, Hiroshi ^b   Morikawa, Kiyoshi ^b   Yoshida, Keiichi ^b   Tahara, Yasutaka ^a  

^a SHIZUOKA UNIVERSITY   (Japan)

^b SEIKAGAKU CORPORATION   (Japan)

Author keywords

Bacillus circulans; Glycosaminoglycan; Heparan sulfate; Heparin; Heparinase

Indexed keywords

HEPARAN SULFATE; HEPARIN; HEPARIN LYASE;

ARTICLE; BACILLUS; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; HYDROLYSIS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR WEIGHT; POLYACRYLAMIDE GEL ELECTROPHORESIS;

BACILLUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HEPARIN; HEPARIN LYASE; HEPARITIN SULFATE; HYDROLYSIS; KINETICS; MOLECULAR WEIGHT; SUBSTRATE SPECIFICITY;

BACILLUS CIRCULANS; BACTERIA (MICROORGANISMS); FLAVOBACTERIUM; PEDOBACTER HEPARINUS;

EID: 0036560772     PISSN: 09168451     EISSN: 13476947     Source Type: Journal    
DOI: 10.1271/bbb.66.1181     Document Type: Article

References (17)

1. Ernst, S., Larger, R., Cooney, C. L., and Sasisekharan, R., Enzymatic degradation of glyco-saminoglycan. Crit. Rev. Biochem. Mol. Biol., 30, 387-444 (1995).
2. Lohse, D. L. and Linhardt, R. J., Purification and characterization of heparin lyases from Flavobacterium heparinum. J. Biol. Chem., 267, 24347-24355 (1992).
3. Yamada, S. and Sugahara, K., Structure of oligosaccharides isolated from heparan sulfate/heparin and substrate specificities of the degrading enzymes of bacterial origin. Trends Glycosci. Glycotechnol., 10, 95-123 (1998).
4. Yang, V. C., Linhardt, R. J., Bernstein, H., Cooney, C. L., and Langer, R., Purification and characterization of heparinase from Flavobacterium heparinum. J. Biol. Chem., 260, 1849-1857 (1985).
5. Watanabe, M., Tsuda, H., Yamada, S., Shibata, Y., Nakamura, T., and Sugahara, K., Characterization of heparinase from an oral bacterium Prevotella heparinolytica. J. Biochem., 123, 283-288 (1998).
6. Kim, B.-H., Kim, W.-S., Kim, Y. S., Linhardt, R. J., and Kim, D.-H., Purification and characterization of a novel heparinase from Bacteroides stercoris HJ-15. J. Biochem., 128, 323-328 (2000).
7. Desai, U. R., Wang, H.-M., and Linhardt, R. J., Substrate specificity of the heparin lyases from Flavobacterium heparinum. Arch. Biochem. Biophys., 306, 461-468 (1993).
8. Sasisekharan, R., Bulmer, M., Moremen, K. W., Cooney, C. L., and Langer, R., Cloning and expression of heparinase I gene from Flavobacterium heparinum. Pro. Natl. Acad. Sci. USA, 90, 3660-3664 (1993).
9. Su, H., Blain, F., Musil, R. A., Zimmermann, J. J. F., Gu, K., and Bennett, D. C., Isolation and expression in Escherichia coli of hepB and hepC, genes coding for the glycosaminoglycan-degrading enzymes heparinase II and heparinase III, respectively, from Flavobacterium heparinum. Appl. Environ. Microbiol., 62, 2723-2734 (1996).
10. Godavarti, R., Davis, M., Venkataraman, G., Cooney, C. L., Langer, R., and Sasisekharann, R., Heparinase III from Flavobacterium heparinum: Cloning and recombinant expression in Escherichia coli. Biochem. Biophys. Res. Comm., 225, 751-758 (1996).
11. Godavarti, R. and Sasisekharan, R., A comparison of the primary sequences and characteristics of heparinases I, II, and III from Flavobacterium hepar-inum. Biochem. Biophys. Res. Comm., 229, 770-777 (1996).
12. Payza, A. N. and Korn, E. D., The degradation of heparin by bacterial enzymes. I. Adaptation and lyophilized cells. J. Biol. Chem., 223, 853-858 (1956).
13. Linhardt, R. J., Fitzgerald, G. L., Cooney, C. L., and Langer, R., Mode of action of heparin lyase on heparin. Biochim. Biophys. Acta, 702, 197-203 (1982).
14. Lowry, O. H., Rosebrough, N. J., and Randall, A. L., Protein measurement with the folin phenol reagent. J. Biol. Chem., 193, 265-275 (1951).
15. Moffat, C. F., McLean, M. W., Long, W. F., and Williamson, F. B., Heparinase II from Flavobacterium heparinum: HPLC analysis of the saccharides generated from chemically modified heparins. Eur. J. Biocchem., 202, 531-541 (1991).
16. Ampofo, S. A., Wang, H. W., and Linhardt, R. J., Disaccharide compositional analysis of heparin and heparan sulfate using capillary zone electrophoresis. Anal. Biochem., 199, 249-255 (1991).
17. Laemmli, U. K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685 (1970).