A novel fluorescence imaging technique combining deconvolution microscopy and spectral analysis for quantitative detection of opportunistic pathogens

Journal of Microbiological Methods

Volumn 67, Issue 3, 2006, Pages 597-602

  Le Puil, M ^a   Biggerstaff, J P ^b   Weidow, B L ^b   Price, J R ^c   Naser, S A ^d   White, D C ^b   Alberte, R S ^a  

^a FLORIDA GULF COAST UNIVERSITY   (United States)

^b UNIVERSITY OF TENNESSEE   (United States)

^c OAK RIDGE NATIONAL LABORATORY   (United States)

^d UNIVERSITY OF CENTRAL FLORIDA   (United States)

Author keywords

Confocal microscopy; Deconvolution; FISH; Pathogens; Spectral imaging

Indexed keywords

ACCURACY; ARTICLE; CLOSTRIDIUM DIFFICILE; CONFOCAL LASER MICROSCOPY; CONTROLLED STUDY; ESCHERICHIA COLI; FLUORESCENCE; FLUORESCENCE IN SITU HYBRIDIZATION; LISTERIA MONOCYTOGENES; MICROORGANISM DETECTION; MYCOBACTERIUM AVIUM; MYCOBACTERIUM AVIUM PARATUBERCULOSIS; MYCOBACTERIUM INTRACELLULARE AVIUM; NONHUMAN; OBSERVER VARIATION; OPPORTUNISTIC INFECTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SPECTROSCOPY;

BACTERIA; CLOSTRIDIUM DIFFICILE; ESCHERICHIA COLI; LISTERIA MONOCYTOGENES; MICROSCOPY, FLUORESCENCE; MYCOBACTERIUM AVIUM COMPLEX; MYCOBACTERIUM PARATUBERCULOSIS; RNA, RIBOSOMAL, 16S; SPECTROMETRY, FLUORESCENCE;

EID: 33750319228     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2006.05.019     Document Type: Article

References (31)

1. Adak G.K., Long S.M., and O'Brien S.J. Trends in indigenous foodborne disease and deaths, England and Wales: 1992 to 2000. Gut 51 (2002) 832-841
2. Amann R.I., Krumholz L., and Stahl D.A. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172 (1990) 762-770
3. Amann R.I., Binder B.J., Olson R.J., Chrisholm S.W., et al. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56 (1990) 1919-1925
4. Barbut F., Corthier G., Charpak Y., Cerf M., Monteil H., Fosse T., Trevoux A., De Barbeyrac B., Boussougant Y., Tigaud S., Tytgat F., Sedallian A., Duborgel S., Collignon A., Le Guern M.E., Bernasconi P., and Petit J.C. Prevalence and pathogenicity of Clostridium difficile in hospitalized patients: a French multicenter study. Arch. Intern. Med. 156 (1996) 1449-1454
5. Biggerstaff J., Amirkhosravi A., and Francis J.L. Three-dimensional visualization and quantitation of fibrin in solid tumors by confocal laser scanning microscopy. Cytometry 29 (1997) 122-127
6. Carrington W.A., Lynch R.M., Moore E.D., Isenberg G., Fogarty K.E., and Fay F.S. Superresolution three-dimensional images of fluorescence in cells with minimal light exposure. Science 268 (1995) 1483-1487
7. Chang Y.C., Le Puil M., Biggerstaff J., Randall A.A., Shulte A., and Taylor J.S. Direct estimation of biofilm density on different pipe material coupons using a specific DNA-probe. Mol. Cell. Probes 17 (2003) 237-243
8. Cohn P.D., Cox M., and Berger P.S. Health and aesthetic aspects of water quality. Water quality and treatment: a handbook of community water supplies, 2-7 and 2-8, American Water Works Association. 5th ed. (1999), McGraw Hill, Inc., New York
9. Collins M.T. Mycobacterium paratuberculosis: a potential food-borne pathogen?. J. Dairy Sci. 80 (1997) 3445-3448
10. Collins D.M., Cavaignac S., and de Lisle G.W. Use of four DNA insertion sequences to characterize strains of the Mycobacterium avium complex isolated from animals. Mol. Cell. Probes. 11 (1997) 373-380
11. Covert T.C., Rodgers M.R., Reyes A.L., and Stelma Jr. G.N. Occurrence of nontuberculous mycobacteria in environmental samples. Appl. Environ. Microbiol. 65 (1999) 2492-2496
12. Du Moulin G.C., and Stottmeier K.D. Waterbrone mycobacteria: an increasing threat to health. ASM News 52 (1986) 525-529
13. Ericsson H., Eklöw A., Danielsson-Tham M.-L., Loncarevic S., Persson I., Unnerstad H., and Tham W. An outbreak of listeriosis suspected to have been caused by rainbow trout. J. Clin. Microbiol. 35 (1997) 2904-2907
14. Food Safety and Inspection Service, U.S. Department of Agriculture., 2002. May 2000. Revised action plan for control of Listeria monocytogenes for the prevention of foodborne listeriosis. Available at: http://www.fsis.usda.gov/OA/topics/lm_action.htm. Accessed 20 Mar. 2006.
15. Franke-Whittle I.H., Klammer S.H., and Insam H. Design and application of an oligonucleotide microarray for the investigation of compost microbial communities. J. Microbiol. Methods 62 (2005) 37-56
16. Furrer B., Candrian U., Hoefelein C., and Luethy J. Detection and identification of Listeria monocytogenes in cooked sausage products and in milk by in vitro amplification of haemolysin gene fragments. J. Appl. Bacteriol. 70 (1991) 372-379
17. Hiraoka Y., Shimi T., and Haraguchi T. Multispectral imaging fluorescence microscopy for living cells. Cell Struct. Funct. 27 (2002) 367-374
18. Huijsdens X.W., Linskens R.K., Mak M., Meuwissen S.G.M., Vandenbroucke-Grauls C.M.J.E., and Savelkoul P.H.M. Quantification of bacteria adherent to gastrointestinal mucosa by real-time PCR. J. Clin. Microbiol. 40 (2002) 4423-4427
19. Kalmbach S., Manz W., and Szewzyk U. Development of a new method to determine the metabolic potential of bacteria in drinking water biofilms: probe active counts (PAC). In: Flemming H.-C., Szewzyk U., and Griebe T. (Eds). Biofilms: investigative methods and applications (2000), Technomic Publishing, Lancaster 107-121
20. Loo V.G., Poirier L., Miller M.A., Oughton M., Libman M.D., Michaud S., Bourgault A.-M., Nguyen T., Frenette C., Kelly M., Vibien A., Brassard P., Fenn S., Dewar K., Hudson T.J., Horn R., René P., Monczak Y., and Dascal A. A predominantly clonal multi-institutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality. N. Engl. J. Med. 353 (2005) 2442-2449
21. Maierhofer C., Gangnus R., Diebold J., and Speicher M.R. Multicolor deconvolution microscopy of thick biological specimens. Am. J. Pathol. 162 (2003) 373-379
22. Manz W., Szewzyk U., Ericsson P., Amann R., Schleifer K.H., and Stenström T.-A. In situ identification of bacteria in drinking water and adjoining biofilms by hybridization with 16S and 23S r RNA-directed fluorescent oligonucleotides probes. Appl. Environ. Microbiol. 59 (1993) 2293-2298
23. Manz W., Gernot A., Schumann-Kindel G., Szewzyk U., and Reitner J. Widefield deconvolution epifluorescence microscopy combined with fluorescence in situ hybridization reveals the spatial arrangement of bacteria in sponge tissue. J. Microbiol. Methods 40 (2000) 125-134
24. McNally J.G., Karpova T., Cooper J., and Conchello J.A. Three-dimensional imaging by deconvolution microscopy. Methods 19 (1999) 373-385
25. Mishina D., Katsel P., Brown S.T., Gilberts E.C., and Greenstein R.J. On the etiology of Crohn's disease. Proc. Natl. Acad. Sci. U. S. A. 93 (1996) 9816-9820
26. Mitchell R. Water pollution microbiology (1972), John Wiley & Sons, New York
27. Naser S.A., Ghobrial G., Romero C., and Valentine J.F. Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn's disease. Lancet 364 (2004) 1039-1044
28. Schröck E., du Manoir S., Veldman T., Schoell B., Wienberg J., Ferguson-Smith M.A., Ning Y., Ledbetter D.H., Bar-Am I., Soenksen D., Garini Y., and Ried T. Multicolor spectral karyotyping of human chromosomes. Science 273 (1996) 494-497
29. Sobsey M., and Olsen B. Microbial agents of waterborne disease. In: assessment of microbiology and turbidity standards for drinking water. In: Berger P.S., and Argamon Y. (Eds). EPA570/9-83-001 (1983), U.S. Environmental Protection Agency, Office of Drinking Water, Washington, D.C.
30. Vives-Rego J., Lebaron P., and Nebe-von Caron G. Current and future applications of flow cytometry in aquatic microbiology. FEMS Microbiol. Rev. 24 (2000) 429-448
31. Von Reyn C.F., Maslow J.N., Barber T., Falkinham III J.O., and Arbeit R.D. Persistent colonisation of potable water as a source of Mycobacterium infection in AIDS. Lancet 343 (1994) 1137-1141