The combined use of non-radioactive in situ hybridization and real-time RT-PCR to assess gene expression in cryosections

Brain Research

Volumn 1118, Issue 1, 2006, Pages 232-238

  Haupt, Corinna ^a   Tolner, Else A ^b   Heinemann, Uwe ^b   Witte, Otto W ^a   Frahm, Christiane ^a  

^a FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Computerized image analysis; Connexin 43; Kainic acid; Seizure; Status epilepticus

Indexed keywords

CONNEXIN 43; DIGOXIGENIN; KAINIC ACID; MESSENGER RNA;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ASTROCYTE; AUTOMATION; BRAIN REGION; CONTROLLED STUDY; EPILEPTIC STATE; EPILEPTOGENESIS; FROZEN SECTION; GAP JUNCTION; GENE EXPRESSION; HIPPOCAMPUS; IMAGE ANALYSIS; IN SITU HYBRIDIZATION; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEIZURE;

ANIMALS; CELL COMMUNICATION; CELL COUNT; CONNEXIN 43; CONVULSANTS; DISEASE MODELS, ANIMAL; EPILEPSY; GAP JUNCTIONS; GENE EXPRESSION; HIPPOCAMPUS; IMAGE CYTOMETRY; IN SITU HYBRIDIZATION; KAINIC ACID; MALE; RATS; RATS, SPRAGUE-DAWLEY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 33750301033     PISSN: 00068993     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.brainres.2006.08.037     Document Type: Article

References (20)

1. Aronica E., Gorter J.A., Jansen G.H., Leenstra S., Yankaya B., and Troost D. Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol. (Berl.) 101 (2001) 449-459
2. Beyer E.C., Paul D.L., and Goodenough D.A. Connexin43: a protein from rat heart homologous to a gap junction protein from liver. J. Cell Biol. 105 (1987) 2621-2629
3. Buckmaster P.S., and Dudek F.E. Neuron loss, granule cell axon reorganization, and functional changes in the dentate gyrus of epileptic kainate-treated rats. J. Comp. Neurol. 385 (1997) 385-404
4. Dinocourt C., Petanjek Z., Freund T.F., Ben Ari Y., and Esclapez M. Loss of interneurons innervating pyramidal cell dendrites and axon initial segments in the CA1 region of the hippocampus following pilocarpine-induced seizures. J. Comp. Neurol. 459 (2003) 407-425
5. Esclapez M., and Houser C.R. Up-regulation of GAD65 and GAD67 in remaining hippocampal GABA neurons in a model of temporal lobe epilepsy. J. Comp. Neurol. 412 (1999) 488-505
6. Frahm C., and Draguhn A. GAD and GABA transporter (GAT-1) mRNA expression in the developing rat hippocampus. Brain Res. Dev. Brain Res. 132 (2001) 1-13
7. Frahm C., Haupt C., Weinandy F., Siegel G., Bruehl C., and Witte O.W. Regulation of GABA transporter mRNA and protein after photothrombotic infarct in rat brain. J. Comp. Neurol. 478 (2004) 176-188
8. Frahm C., Haupt C., and Witte O.W. GABA neurons survive focal ischemic injury. Neuroscience 127 (2004) 341-346
9. Hellier J.L., Patrylo P.R., Dou P., Nett M., Rose G.M., and Dudek F.E. Assessment of inhibition and epileptiform activity in the septal dentate gyrus of freely behaving rats during the first week after kainate treatment. J. Neurosci. 19 (1999) 10053-10064
10. Hill C.E., Rummery N., Hickey H., and Sandow S.L. Heterogeneity in the distribution of vascular gap junctions and connexins: implications for function. Clin. Exp. Pharmacol. Physiol. 29 (2002) 620-625
11. Hunsberger J.G., Bennett A.H., Selvanayagam E., Duman R.S., and Newton S.S. Gene profiling the response to kainic acid induced seizures. Brain Res. Mol. Brain Res. 141 (2005) 95-112
12. Khurgel M., and Ivy G.O. Astrocytes in kindling: relevance to epileptogenesis. Epilepsy Res. 26 (1996) 163-175
13. Kobori H., Harrison-Bernard L.M., and Navar L.G. Enhancement of angiotensinogen expression in angiotensin II-dependent hypertension. Hypertension 37 (2001) 1329-1335
14. Margaill I., Royer J., Lerouet D., Ramauge M., Le Goascogne C., Li W.W., Plotkine M., Pierre M., and Courtin F. Induction of type 2 iodothyronine deiodinase in astrocytes after transient focal cerebral ischemia in the rat. J. Cereb. Blood Flow Metab. 25 (2005) 468-476
15. Nash J.E., Johnston T.H., Collingridge G.L., Garner C.C., and Brotchie J.M. Subcellular redistribution of the synapse-associated proteins PSD-95 and SAP97 in animal models of Parkinson's disease and l-DOPA-induced dyskinesia. FASEB J. 19 (2005) 583-585
16. Naus C.C., Bechberger J.F., and Paul D.L. Gap junction gene expression in human seizure disorder. Exp. Neurol. 111 (1991) 198-203
17. Pfaffl M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29 (2001) e45
18. Racine R.J. Modification of seizure activity by electrical stimulation: II. Motor seizure. Electroencephalogr. Clin. Neurophysiol. 32 (1972) 281-294
19. Sohl G., Guldenagel M., Beck H., Teubner B., Traub O., Gutierrez R., Heinemann U., and Willecke K. Expression of connexin genes in hippocampus of kainate-treated and kindled rats under conditions of experimental epilepsy. Brain Res. Mol. Brain Res. 83 (2000) 44-51
20. Tolner E.A., van Vliet E.A., Holtmaat A.J., Aronica E., Witter M.P., da Silva F.H., and Gorter J.A. GAP-43 mRNA and protein expression in the hippocampal and parahippocampal region during the course of epileptogenesis in rats. Eur. J. Neurosci. 17 (2003) 2369-2380