Stored human septal chondrocyte viability analyzed by confocal microscopy

Archives of Otolaryngology - Head and Neck Surgery

Volumn 132, Issue 10, 2006, Pages 1137-1142

  Hicks, David L ^a,c,d   Sage, August B ^b   Schumacher, Barbara L ^b   Jadin, Kyle D ^b   Agustin, Ramses M ^b   Sah, Robert L ^b   Watson, Deborah ^a,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^c VA SAN DIEGO HEALTHCARE SYSTEM   (United States)

^d VETERANS AFFAIRS MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; ARTICLE; CARTILAGE CELL; CELL VIABILITY; CLINICAL ARTICLE; HUMAN; HUMAN CELL; MEDICAL RESEARCH; NOSE CARTILAGE; NOSE SEPTUM; STORAGE; TEMPERATURE; TISSUE ENGINEERING;

ADULT; CELL SURVIVAL; CHONDROCYTES; HUMANS; MICROSCOPY, CONFOCAL; MIDDLE AGED; NASAL SEPTUM; SODIUM CHLORIDE; TEMPERATURE; TISSUE ENGINEERING; TISSUE PRESERVATION;

EID: 33750190613     PISSN: 08864470     EISSN: 08864470     Source Type: Journal    
DOI: 10.1001/archotol.132.10.1137     Document Type: Article

References (22)

1. Benya PD, Shaffer J. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell. 1982;30:215-224.
2. Vacanti CA, Langer R, Schloo B, Vacanti JP. Synthetic polymers seeded with chondrocytes provide a template for new cartilage formation. Plast Reconstr Surg. 1991;88:753-759.
3. Vacanti CA, Paige KT, Kim WS, Sakata J, Upton J, Vacanti JP. Experimental tracheal replacement using tissue-engineered cartilage. J Pediatr Surg. 1994;29:201-204.
4. Lee CJ, Moon KD, Choi H, Woo JI, Min BH, Lee KB. Tissue engineered tracheal prosthesis with acceleratedly cultured homologous chondrocytes as an alternative of tracheal reconstruction. J Cardiovasc Surg (Torino). 2002;43:275-279.
5. van Osch GJ, van der Veen SW, Verwoerd-Verhoef HL. In vitro redifferentiation of culture-expanded rabbit and human auricular chondrocytes for cartilage reconstruction. Plast Reconstr Surg. 2001;107:433-440.
6. Rotter N, Tobias G, Lebl M, et al. Age-related changes in the composition and mechanical properties of human nasal cartilage. Arch Biochem Biophys. 2002;403:132-140.
7. Koch RJ, Gorti GK. Tissue engineering with chondrocytes. Facial Plast Surg. 2002;18:59-68.
8. Guyuron B, Friedman A. The role of preserved autogenous cartilage graft in septorhinoplasty. Ann Plast Surg. 1994;32:255-260.
9. Sancho BV, Molina AR. Use of septal cartilage homografts in rhinoplasty. Aesthetic Plast Surg. 2000;24:357-363.
10. Bujia J, Dremer D, Sudhoff H, Viviente E, Sprekelsen C, Wilmes E. Determination of viability of cryopreserved cartilage grafts. Eur Arch Otorhinolaryngol. 1995;252:30-34.
11. Bujia J, Pitzke P, Wilmes E, Hammer C. Culture and cryopreservation of chondrocytes from human cartilage: relevance for cartilage allografting in otolaryngology. ORL J Otorhinolaryngol Relat Spec. 1992;54:80-84.
12. Cao L, Lee V, Adams ME, et al. Beta-integrin-collagen interaction reduces chondrocyte apoptosis. Matrix Biol. 1999;18:343-355.
13. Lo MY, Kim HT. Chondrocyte apoptosis induced by collagen degradation: inhibition by caspase inhibitors and IGF-1. J Orthop Res. 2004;22:140-144.
14. Takahashi T, Yamamoto H, Ogawa Y, et al. Role of apoptosis inhibition in various chondrocyte culture systems. Int J Mol Med. 2003;11:299-303.
15. Kuhn K, Shikhman AR, Lotz M. Role of nitric oxide, reactive oxygen species, and p38 MAP kinase in the regulation of human chondrocyte apoptosis. J Cell Physiol. 2003;197:379-387.
16. Paddock SW. Confocal laser scanning microscopy. Biotechniques. 1999;27:992-1004.
17. Breuls RGM, Mol A, Petterson R, Oomens CWJ, Baaijens FPT, Bouten CVC. Monitoring local cell viability in engineered tissues: a fast, quantitative, and nondestructive approach. Tissue Eng. 2003;9:269-281.
18. Lopez-Amoros R, Castel S, Comas-Riu J, Vives-Rego J. Assessment of E. coli and Salmonella viability and starvation by confocal laser microscopy and flow cytometry using rhodamine 123, BiBAC4(3), propidium iodide, and CTC. Cytometry. 1997;29:298-305.
19. Takeuchi K, Frank JF. Confocal microscopy and microbial viability detection for food research. J Food Prot. 2001;64:2088-2102.
20. Monette R, Small DL, Mealing F, Morley P. A fluorescence confocal assay to assess neuronal viability in brain slices. Brain Res Brain Res Protoc. 1998;2:99-108.
21. Aigner J, Hutzler P, Bujia J, Kastenbauer E. Distribution and viability of cultured human chondrocytes in a three-dimensional matrix as assessed by confocal laser scan microscopy. In Vitro Cell Dev Biol Anim. 1997;33:407-409.
22. Lasczkowski GE, Aigner T, Gamerdinger U, Weiler G, Bratzke H. Visualization of postmortem chondrocyte damage by vital staining and confocal laser scanning 3D microscopy. J Forensic Sci. 2002;47:663-666.