Protein-DNA interaction-based detection of small molecules by employing Renilla luciferase fusion protein: Quantitative and generic measurement of tetracyclines with a Renilla luciferase-tagged Tet repressor protein

Analytical Biochemistry

Volumn 358, Issue 2, 2006, Pages 301-303

  Pellinen, Teijo ^a   Rytkonen, Kalle ^a   Ristiniemi, Noora ^a   Karp, Matti ^a,b   Lamminmaki, Urpo ^a  

^a UNIVERSITY OF TURKU   (Finland)

^b TAMPERE UNIVERSITY OF TECHNOLOGY   (Finland)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CHEMISTRY;

FUSION PROTEINS; PROTEIN-DNA INTERACTIONS; RENILLA LUCIFERASE; SMALL MOLECULES;

PROTEINS;

DNA; LUCIFERASE; PROTEIN; RENILLA LUCIFERIN 2 MONOOXYGENASE; TETRACYCLINE DERIVATIVE;

ALLOSTERISM; ARTICLE; BACTERIAL PHENOMENA AND FUNCTIONS; DNA BINDING; ESCHERICHIA COLI; GENE FUSION; GENETIC TRANSCRIPTION; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN INTERACTION; REPRESSOR GENE; SIGNAL TRANSDUCTION;

RENILLA LUCIFERASE;

EID: 33750028541     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2006.08.008     Document Type: Article

References (11)

1. Pancrazio J.J., Whelan J.P., Borkholder D.A., Ma W., and Stenger D.A. Development and application of cell-based biosensors. Ann. Biomed. Eng. 27 (1999) 697-711
2. Lederer T., Kintrup M., Takahashi M., Sum P.E., Ellestad G.A., and Hillen W. Tetracycline analogs affecting binding to Tn10-encoded Tet repressor trigger the same mechanism of induction. Biochemistry 35 (1996) 7439-7446
3. Hillen W., and Berens C. Mechanisms underlying expression of Tn10-encoded tetracycline resistance. Annu. Rev. Microbiol. 48 (1994) 345-369
4. Berens C., and Hillen W. Gene regulation by tetracyclines: Constraints of resistance regulation in bacteria shape TetR for application in eukaryotes. Eur. J. Biochem. 270 (2003) 3109-3121
5. Korpela M.T., Kurittu J.S., Karvinen J.T., and Karp M.T. A recombinant Escherichia coli sensor strain for the detection of tetracyclines. Anal. Chem. 70 (1998) 4457-4462
6. Pellinen T., Huovinen T., and Karp M. A cell-free biosensor for the detection of transcriptional inducers using firefly luciferase as a reporter. Anal. Biochem. 330 (2004) 52-57
7. Weber C.C., Link N., Fux C., Zisch A.H., Weber W., and Fussenegger M. Broad-spectrum protein biosensors for class-specific detection of antibiotics. Biotechnol. Bioeng. 8 (2005) 9-17
8. Takahashi M., Altschmied L., and Hillen W. Kinetic and equilibrium characterization of the Tet repressor-tetracycline complex by fluorescence measurements: Evidence for divalent metal ion requirement and energy transfer. J. Mol. Biol. 187 (1986) 341-348
9. Promega, Renilla luciferase assay system, in: Technical Manual No. 055, Promega, Madison, WI, 2005, p. 2.
10. Välimaa L., Pettersson K., Vehniäinen M., Karp M., and Lövgren T. A high-capacity streptavidin-coated microtitration plate. Bioconj. Chem. 14 (2003) 103-111
11. Pellinen T., Bylund G., Virta M., Niemi A., and Karp M. Detection of traces of tetracyclines from fish with a bioluminescent sensor strain incorporating bacterial luciferase reporter genes. J. Agric. Food Chem. 50 (2002) 4812-4815