Can you trust your cryostat? Reproducibility of cryostat section thickness

Microscopy Research and Technique

Volumn 69, Issue 10, 2006, Pages 835-838

  De Witt Hamer, Philip C ^a   Bleeker, Fonnet E ^a   Zwinderman, Aeilko H ^a   Van Noorden, Cornelis J F ^a  

^a UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Cryomicrotome; Cryostat; Frozen sections; Lactate dehydrogenase activity; Quantitative microscopy; Reproducibility; Section thickness

Indexed keywords

ABSORBANCE MEASUREMENTS; COEFFICIENTS OF VARIATIONS; CONSTANT SPEED; CRYOMICROTOME; FROZEN SECTIONS; LACTATE DEHYDROGENASE ACTIVITIES; QUANTITATIVE MICROSCOPY; REPRODUCIBILITIES; SECTION THICKNESS; SERIAL SECTION;

CRYOSTATS;

LACTATE DEHYDROGENASE;

ANIMAL TISSUE; APPARATUS; ARTICLE; ENZYME ACTIVITY; FROZEN SECTION; HISTOCHEMISTRY; INTERMETHOD COMPARISON; MEASUREMENT; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; RAT; REPRODUCIBILITY; THICKNESS; TISSUE HOMOGENATE;

EID: 33750020867     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20354     Document Type: Article

References (11)

1. Anthony A, Colurso GJ, Bocan TM, Doebler JA. 1984. Interferometric analysis of intrasection and intersection thickness variability associated with cryostat microtomy. Histochem J 16:61-70.
2. Butcher RG. 1971. The chemical determination of section thickness. Histochemie 28:131-136.
3. Butcher RG. 1972. Precise cytochemical measurement of neotetrazolium formazan by scanning and integrating microdensitometry. Histochemie 32:171-190.
4. Cabrini RL, Folco A, Orrea S, Savino MT, Schwint AM, Itoiz ME. 1998. A technique for section thickness evaluation for microphotometry and image analysis of sectioned nuclei. Anal Cell Pathol 17:125-130.
5. De Witt Hamer PC, Jonker A, Leenstra S, Ruijter JM, Van Noorden CJ. 2005. Quantification of viability in organotypic multicellular spheroids of human malignant glioma using lactate dehydrogenase activity: A rapid and reliable automated assay. J Histochem Cytochem 53:23-34.
6. Gschwendtner A, Mairinger T. 1995. How thick is your section? The influence of section thickness on DNA-cytometry on histological sections. Anal Cell Pathol 9:29-37.
7. Halkjaer-Kristensen J, Ingemann-Hansen T. 1978. Thickness measurements of skeletal muscle sections using the light microscope. Histochem J 10:497-504.
8. Pearse AD, Marks R. 1974. Measurement of section thickness in quantitative microscopy with special reference to enzyme histochemistry. J Clin Pathol 27:615-618.
9. Troyer H, Fisher OT, Rosenquist TH. 1977. Bone alkaline phosphatase kinetics studied by a new method. Histochemistry 50:251-259.
10. Van Noorden CJ, Butcher RG. 1987. Linearity in dehydrogenase reaction rate studies in tissue sections is affected by loss of endogenous substrates during the reaction. J Histochem Cytochem 35:1401-1404.
11. Van Noorden CJ, Butcher RG. 1991. Quantitative enzyme histochemistry. In: Stoward PJ, Pearse AGE, editors. Histochemisty, Vol. 3. London, Churchill Livingstone. pp 355-432.