A technique for section thickness evaluation for microphotometry and image analysis of sectioned nuclei

Analytical Cellular Pathology

Volumn 17, Issue 2, 1998, Pages 125-130

  Cabrini, R L ^b   Folco, A ^b   Orrea, S ^b   Savino, M T ^b   Schwint, A M ^b   Itoiz, M E ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b NONE

Author keywords

DNA cytometry; Image analysis; Section thickness

Indexed keywords

ARTICLE; CELL NUCLEUS; DNA DETERMINATION; IMAGE ANALYSIS; OPTICAL DENSITY; PHOTOMETRY; PRIORITY JOURNAL; TISSUE SECTION;

EID: 0032430703     PISSN: 09218912     EISSN: None     Source Type: Journal    
DOI: 10.1155/1998/607373     Document Type: Article

References (13)

1. R.T. Allison and J.F. Vincent, Measuring the forces acting during microtomy by the use of load cells, J. Micros. 159 (1990), 203-210.
2. A. Anthony, G.J. Colurso, T.M.A. Bocan and J.A. Doebler, Interferometric analysis of intrasection and intersection thickness variability associated with cryostat microtomy, Histochemical J. 16 (1984), 61-60.
3. M. Bins and F. Takens, A method to estimate the DNA content of whole nuclei from measurements made on thin tissue sections, Cytometry 6 (1985), 234-237.
4. K.A. Chaubel, Z. Ledin and J. Pilny, A new approach to the determination of thickness of the biological specimens by two wavelength method using interference microscopy, Acta Histochem. (1976), 131-143.
5. G.D. Davies, Estimation of number and diameter of isodiametric spherical particles in microtome sections, J. Micros. 98 (1972), 79-83.
6. Y.S. Fu and T.L. Hall, DNA plody measurements in tissue sections, Anal. Quant. Cytol. 7 (1985), 90-96.
7. A. Gschwendtner, A. Kreczy, K. Prein, C. Schonthaler and T. Mairinger, DNA cytometry: diploid standard and section thickness, Am. J. Clin. Pathol. 102 (1994), 558-559.
8. A. Gschwendtner and T. Mairinger, How thick is your section? The influence of section tickness on DNA-cytometry on histological sections, Analytical Cell Pathol. 9 (1995), 29-37.
9. T. Mairinger, A. Gschwendtner, G. Mikuz and V. Kempf, Tissue section image analysis. Comparison of different software releases, Am. J. Clin. Pathol. 101 (1994), 673.
10. A.D. Pearse and R. Marks, Further studies on section thickness measurement, Histochemical J. 8 (1976), 383-386.
11. J.P. Rigaut and A. Persoz, The "corpuscle" stereological problem. Re-evaluation using slab fragment volumes and application to the correction of DNA histograms from sections of spherical nuclei, J. Micros. 156 (1989), 371-382.
12. Z. Sapi, J.B. Hendricks, P.G. Pharis and E.J. Wilkinson, Tissue section image anlysis of breast neoplasms, Anat. Pathol. 99 (1993), 714-720.
13. H. Schimmelpenning, K. Hamper and U.G. Falkmer, Methodologic aspects of DNA assessment by means of image cytometry in tumours of the salivary glands. A comparison between the results obtained using sections and cytospin preparations from the same paraffin embedded specimens, Anal. Quant. Cytol. 11 (1989), 379-383.