Production of recombinant proteins by sol-gel immobilized Escherichia coli

Enzyme and Microbial Technology

Volumn 40, Issue 1, 2006, Pages 168-171

  Desimone, M F ^a   De Marzi, M C ^a   Copello, G J ^a   Fernandez M M ^a   Pieckenstain, F L ^b   Malchiodi, E L ^a   Diaz, L E ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

^b UNIVERSITY OF GRANADA   (Spain)

Author keywords

Escherichia coli; Immobilization; Preservation; Silicon oxide matrix; Sol gel

Indexed keywords

GEL FILTRATION; RECOMBINANT PROTEIN; STREPTOCOCCAL SUPERANTIGEN (SSA); T-CELL PROLIFERATION;

BIOASSAY; CELL CULTURE; ENZYME IMMOBILIZATION; ESCHERICHIA COLI; SILICA; SOL-GELS;

PROTEINS;

GLYCEROL; RECOMBINANT PROTEIN; SILICON DIOXIDE; STREPTOCOCCUS ANTIGEN; TETRAETHOXYSILANE;

ARTICLE; BACTERIUM CULTURE; BIOSENSOR; CELL PROLIFERATION; CELL VIABILITY; CONTROLLED STUDY; ESCHERICHIA COLI; GEL FILTRATION; HUMAN; HUMAN CELL; NONHUMAN; PRESERVATION; QUANTITATIVE ANALYSIS; T LYMPHOCYTE;

ESCHERICHIA COLI;

EID: 33749677676     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2005.11.052     Document Type: Article

References (14)

1. Armon R., Dosoretz C., Starosvetsky J., Orshansky F., and Saadi I. Sol-gel applications in environmental biotechnology. J Biotechnol 51 (1996) 279-285
2. Premkumar J.R., Lev O., Rosen R., and Belkin S. Encapsulation of luminous recombinant E. coli in sol-gel silicate films. Adv Mater 13 (2001) 1773-1775
3. Böttcher H., Soltmann U., Mertig M., and Pompe W. Biocers: ceramics with incorporated microorganisms for biocatalytic, biosorptive and functional materials development. J Mater Chem 14 (2004) 2175-2188
4. Coiffier A., Coradin T., Roux C., Bouvet O., and Livage J. Sol-gel encapsulation of bacteria: a comparison between alkoxide and aqueous routes. J Mater Chem 11 (2001) 2039-2044
5. Conroy J.F.T., Power M.E., Martin J., Earp B., Hosticka B., Daitch C.E., et al. Cells in sol-gels I: a cytocompatible route for the production of macroporous silica gels. J Sol-gel Sci Tech 18 (2000) 268-283
6. Nassif N., Bouvet O., Rager M.N., Roux C., Coradin T., and Livage J. Living bacteria in silica gels. Nat. Mater. 1 (2002) 42-44
7. Desimone M.F., De Marzi M.C., Copello G.J., Fernández M.M., Malchiodi E.M., and Diaz L.E. Efficient preservation in a silicon oxide matrix of Escherichia coli producer of recombinant proteins. Appl Microbiol Biotechnol 68 (2005) 747-752
8. Fields B.A., Malchiodi E.L., Li H., Isern X., Stauffacher C.V., Schlievert P.M., et al. Crystal structure of a T-cell receptor beta-chain complexed with a superantigen. Nature 384 (1996) 188-192
9. Ward E.S. Secretion of T-cell receptor fragments from recombinant Escherichia coli cells. J Mol Biol 224 (1992) 885-890
10. De Marzi M.C., Fernández M.M., Sundberg E.J., Molinero L., Zwirner N.W., Llera A.S., et al. Cloning, expression and interaction of human T-cell receptors and bacterial SAg SSA. Eur J Biochem 271 (2004) 4075-4083
11. Petersson K., Hakansson M., Nilsson H., Forsberg G., Svensson L.A., Liljas A., et al. EMBO J 20 (2001) 3306-3312
12. Fernández M., De Marzi M., Berguer P., Burzyn D., Langley R., Piazzon I., et al. Mol Immunol (July 12, 2005) [Epublication ahead of print]
13. Desimone M.F., Degrossi J.D., Aquino M., and Diaz L.E. Ethanol tolerance in free and sol-gel immobilised Saccharomyces cerevisiae. Biotechnol Lett 24 (2002) 1557-1559
14. Hilge-Rotmann B., and Rehm H.J. Relationship between fermentation capability and fatty acid composition of free and immobilized Saccharomyces cerevisiae. Appl Microbiol Biotechnol 34 (1991) 502-508