Promoter analysis by saturation mutagenesis

Biological Procedures Online

Volumn 3, Issue 1, 2001, Pages 64-69

  Baliga, Nitin S ^a  

^a INSTITUTE FOR SYSTEMS BIOLOGY   (United States)

Author keywords

Mutagenesis; Polymerase chain reaction; Promoter regions (genetics)

Indexed keywords

EID: 33749626820     PISSN: 14809222     EISSN: None     Source Type: Journal    
DOI: 10.1251/bpo24     Document Type: Article

References (13)

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10. Patenge N, Haase A, Bolhuis H, Oesterhelt D. The gene for a halophilic beta-galactosidase (bgaH) of Haloferax alicantei as a reporter gene for promoter analyses in Halobacterium salinarum. Mol Microbiol. 2000;36(1):105-113.
11. Palmer JR, Daniels CJ. In vivo definition of an archaeal promoter. J Bacteriol. 1995;177(7):1844-1849.
12. Reiter WD, Hudepohl U, Zillig W. Mutational analysis of an archaebacterial promoter: essential role of a TATA box for transcription efficiency and start-site selection in vitro. Proc Natl Acad Sci U S A. 1990;87(24):9509-9513.
13. Yang CF, Kim JM, Molinari E, DasSarma S. Genetic and topological analyses of the bop promoter of Halobacterium halobium: stimulation by DNA supercoiling and non-B-DNA structure. J Bacteriol. 1996;178(3):840-845.