The gene for a halophilic β-galactosidase (bgaH) of Haloferax alicantei as a reporter gene for promoter analyses in Halobacterium salinarum

Molecular Microbiology

Volumn 36, Issue 1, 2000, Pages 105-113

  Patenge, Nadja ^a   Haase, Andrea ^a   Bolhuis, Henk ^a,b   Oesterhelt, Dieter ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE;

ARTICLE; COLORIMETRY; ENZYME ACTIVITY; EXPRESSION VECTOR; GENE EXPRESSION; GENETIC TRANSFORMATION; HALOBACTERIUM SALINARUM; HALOFERAX; NORTHERN BLOTTING; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

BETA-GALACTOSIDASE; GENE EXPRESSION REGULATION, ARCHAEAL; GENES, ARCHAEAL; GENES, REPORTER; GENETIC VECTORS; HALOBACTERIUM; HALOFERAX; PROMOTER REGIONS (GENETICS); RNA, ARCHAEAL; RNA, MESSENGER; TRANSFORMATION, BACTERIAL;

ARCHAEA; HALOBACTERIUM SALINARUM; HALOFERAX; HALOFERAX LUCENTENSE; PROKARYOTA;

EID: 0034074062     PISSN: 0950382X     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2958.2000.01831.x     Document Type: Article

References (31)

1. Abe, K., Ko, M.D.H., and Macgregor, G.R. (1998) A Systematic Molecular genetic Approach to study mammalian germline development. Int J Dev Biol 42: 1051-1065.
2. Bullock, W.O., Fernandez, J.M., and Short, J.M. (1987) XL1-Blue: a High Efficiency Plasmid Transforming recA Escherichia coli Strain with Beta-Galactosidase Selection. Biotechniques 4: 376-379.
3. Chaffin, D.O., and Rubens, C.E. (1998) Blue/white screening of recombinant plasmids in gram-positive Bacteria by interruption of alkaline phosphatase gene (phoZ) expression. Gene 219: 91-99.
4. Chomczynski, P., and Sacchi, N. (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156-159.
5. Cline, S.W., Lam, W.L., Charlebois, R.L., Schalkwyk, L.C., and Doolittle, W.F. (1989) Transformation methods for halophilic archaebacteria. Can J Microbiol 35: 148-152.
6. Danner, S., and Soppa, J. (1996) Characterization of the distal promoter element of halobacteria in vivo using saturation mutagenesis and selection. Mol Microbiol 19: 1265-1276.
7. Ferrando, E., Schweiger, U., and Oesterhelt, D. (1993) Homologous bacterio-opsin-encoding gene expression via site-specific vector integration. Gene 125: 41-47.
8. Gibson, J.L., and Tabita, F.R. (1996) The molecular regulation of the reductive pentose phosphate pathway in proteobacteria and cyanobacteria. Arch Microbiol 166: 141-150.
9. Gropp, F., and Betlach, M.C. (1994) The bat gene of Halobacterium halobium encodes a transacting oxygen inducibility factor. Proc Natl Acad Sci USA 91: 5475-5479.
10. Gropp, F., Gropp, R., and Betlach, M.C. (1995) Effects of upstream deletions on light-and oxygen-regulated bacterioopsin gene expression in Halobacterium halobium. Mol Microbiol 16: 357-364.
11. Hehl, A., Vassella, E., Braun, R., and Roditi, I. (1994) A conserved stem-loop structure in the 3'untranslated region of procyclin mRNAs regulates expression in Trypanosoma brucei. Proc Natl Acad Sci USA 91: 370-374.
12. Holmes, M.L., and Dyall-Smith, M.L. (2000) Sequence and expression of a halobacterial β-galactosidase gene. Mol Microbiol 36: 114-122.
13. Holmes, M.L., Scopes, R.K., Moritz, R.L., Simpson, R.J., Englert, C., Pfeifer, F., et al. (1997) Purification and analysis of an extremely halophilic β-galactosidase from Haloferax alicantei. Biochim Biophys Acta 1337: 276-286.
14. Krebs, M.P., Hauss, T., Heyn, M.P., RajBhandary, U.L, and Khorana, H.G. (1991) expression of the bacterioopsin gene in Halobacterium halobium using a multicopy plasmid. Proc Natl Acad Sci USA 88: 859-863.
15. Meyer, B.J., Bartman, A.E., and Schottel, J.L. (1996) Isolation of a mRNA instability sequence that is cis-dominant to the ompA stability derminant in Escherichia coli. Gene 179: 263-270.
16. Oesterhelt, D., and Krippahl, G. (1983) Phototrophic growth of halobacteria and its use for isolation of photosynthetically-deficient mutants. Ann Microbiol 134B: 137-150.
17. Oesterhelt, D., and Stoeckenius, W. (1973) Functions of a New Photoreceptor Membrane. Proc Natl Acad Sci USA 70: 2853-2857.
18. Pfeiffer, M., Rink, T., Gerwert, K., Oesterhelt, D., and Steinhoff, H.-J. (1999) Site-Directed Spin Labeling Reveals the Orientation of the Amino Acid Side Chains in the E-F Loop of Bacteriorhodopsin. J Mol Biol 287: 163-171.
19. Rist, M., and Kertesz, M.A. (1998) Construction of improved plasmid vectors for promoter characterization in Pseudomonas aerruginosa and other gram-negative bacteria. FEMS Microbiol Lett 169: 179-183.
20. Rothofsky, M.L, and Lin, S.L. (1997) CROC-1 encodes a protein which mediates transcriptional activation of the human FOS promotor. Gene 195: 141-149.
21. Rott, R., Liveanu, V., Drager, R.G., Stern, D.B., and Schuster, G. (1998) The sequence and structure of the 3'untranslated region of chloroplast transcripts are improtant determinants of mRNA accumulation and stability. Plant Mol Biol 36: 307-314.
22. Rudolph, J., and Oesterhelt, D. (1995) Chemotaxis and phototaxis require a CheA histidine kinase in the archaeon Halobacterium salinarum. EMBO J 14: 667-673.
23. Ruepp, A., and Soppa, J. (1996) Fermentative arginine degradation in Halobacterium salinarium - genes, gene products, and transcripts of the arcRACB gene cluster. J Bacteriol 178: 4942-4947.
24. Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
25. Shand, R.F., and Betlach, M.C. (1991) Expression of the bop gene cluster of Halobacterium halobium is induced by low oxygen tension and by light. J Bacteriol 173: 4692-4699.
26. Shand, R.F., and Betlach, M.C. (1994) bop gene cluster expression in bacteriorhodopsin-overproducing Mutants of Halobacterium halobium. J Bacteriol 176: 1655-1660.
27. Sirard, J.C., Mock, M., and Fouet, A. (1995) Molecular tools for the study of transcriptional regulation in Bacillus anthracis. Res Microbiol 146: 729-737.
28. Sniezko, I., Dobson-Stone, C., and Klein, A. (1998) The treA gene of Bacillus subtilis is a suitable reporter gene for the archaeon Methanococcus voltae. FEMS Microbiol Lett 164: 237-242.
29. Stoeckenius, W. (1979) Bacteriorhodopsin and the purple membrane of Halobacteria. Biochim Biophys Act 505: 215-278.
30. Sumper, M., and Herrmann, G. (1976) Biosynthesis of purple membrane: control of retinal synthesis by bacterio-opsin. FEBS Microbiol Lett 71: 333-336.
31. Tam, P.P.L., Williams, E.A., and Tan, S.S. (1994) Expression of an x-linked hmg-lacZ transgene in mouse embryos - implications of chromosomal imprinting and lineage-specific x-chromosome activity. Dev Genet 15: 491-503.