Vaccination with recombinant adhesins from the RgpA-Kgp proteinase-adhesin complex protects against Porphyromonas gingivalis infection

Vaccine

Volumn 24, Issue 42-43, 2006, Pages 6542-6554

  Frazer, Leanne T ^a   O'Brien Simpson, Neil M ^a   Slakeski, Nada ^a   Walsh, Katrina A ^a   Veith, Paul D ^a   Chen, Chao Guang ^b   Barr, Ian G ^b   Reynolds, Eric C ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b CSL LIMITED   (Australia)

Author keywords

Porphyromonas gingivalis; Recombinant Adhesins; Vaccination

Indexed keywords

ADHESIN; BACTERIAL VACCINE; PROTEINASE; RECOMBINANT PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL INFECTION; CONTROLLED STUDY; FLOW CYTOMETRY; IMMUNIZATION; IMMUNOREACTIVITY; MOUSE; NONHUMAN; OUTER MEMBRANE; PORPHYROMONAS GINGIVALIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN MOTIF; SEROTYPE; STATISTICAL SIGNIFICANCE; WESTERN BLOTTING; WHOLE CELL;

ADHESINS, BACTERIAL; ANIMALS; BACTERIAL OUTER MEMBRANE PROTEINS; BACTERIAL VACCINES; BACTEROIDACEAE INFECTIONS; BLOTTING, WESTERN; CATALYSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FLOW CYTOMETRY; IMMUNIZATION; MICE; MICE, INBRED BALB C; PEPTIDE HYDROLASES; PEPTIDE MAPPING; PORPHYROMONAS GINGIVALIS; VACCINES, SYNTHETIC;

EID: 33749435760     PISSN: 0264410X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vaccine.2006.06.013     Document Type: Article

References (27)

1. O'Brien-Simpson N.M., Veith P.D., Dashper S.G., and Reynolds E.C. Antigens of bacteria associated with periodontitis. Periodontol 2000 35 (2004) 1-34
2. O'Brien-Simpson N.M., Veith P.D., Dashper S.G., and Reynolds E.C. Porphyromonas gingivalis gingipains: the molecular teeth of a microbial vampire. Curr Protein Pept Sci 4 6 (2003) 409-426
3. Persson G.R., Engel D., Whitney G., Darveau R., Weinberg A., Brunsvold M., et al. Immunization against Porphyromonas gingivalis inhibits progression of experimental periodontitis in non-human primates. Infect Immun 62 (1994) 1026-1031
4. Sutterwala F.S., Noel G.J., Salgame P., and Mosser D.M. Reversal of proinflammatory responses by ligating the macrophage Fcγ receptor. J Exp Med 188 1 (1998) 217-222
5. Fanger N.A., Voigtlaender D., Liu C., Swink S., Wardwell K., Fisher J., et al. Characterization of expression, cytokine regulation, and effector function of the high affinity IgG receptor Fc gamma RI (CD64) expressed on human blood dendritic cells. J Immunol 158 7 (1997) 3090-3098
6. Davis W., Harrison P.T., Hutchinson M.J., and Allen J.M. Two distinct regions of FC gamma RI initiate separate signalling pathways involved in endocytosis and phagocytosis. Embo J 14 3 (1995) 432-441
7. Curtis M.A., Kuramitsu H.K., Lantz M., Macrina F.L., Nakayama K., Potempa J., et al. Molecular genetics and nomenclature of proteases of Porphyromonas gingivalis. J Periodontal Res 34 8 (1999) 464-472
8. Bhogal P.S., Slakeski N., and Reynolds E.C. A cell-associated protein complex of Porphyromonas gingivalis W50 composed of Arg- and Lys-specific cysteine proteinases and adhesins. Microbiology 143 (1997) 2485-2495
9. Slakeski N., Cleal S.M., Bhogal P.S., and Reynolds E.C. Characterization of a Porphyromonas gingivalis gene prtK that encodes a lysine-specific cysteine proteinase and three sequence-related adhesins. Oral Microbiol Immunol 14 2 (1999) 92-97
10. Veith P.D., Talbo G.H., Slakeski N., Dashper S.G., Moore C., Paolini R.A., et al. Major outer membrane proteins and proteolytic processing of RgpA and Kgp of Porphyromonas gingivalis W50. Biochem J 363 Part 1 (2002) 105-115
11. Slakeski N., Bhogal P.S., O'Brien-Simpson N.M., and Reynolds E.C. Characterisation of a second cell-associated Arg-specific cysteine proteinase of Porphyromonas gingivalis and identification of an adhesin binding motif involved in association of the PrtR and PrtK proteinases and adhesins into large complexes. Microbiology 144 (1998) 1583-1592
12. O'Brien-Simpson N., Paolini R., and Reynolds E. RgpA-Kgp peptide-based immunogens provide protection against Porphyromonas gingivalis challenge in a murine lesion model. Infect Immun 68 7 (2000) 4055-4063
13. Rajapakse P.S., O'Brien-Simpson N.M., Slakeski N., Hoffmann B., and Reynolds E.C. Immunization with the RgpA-Kgp proteinase-adhesin complexes of Porphyromonas gingivalis protects against periodontal bone loss in the rat periodontitis model. Infect Immun 70 5 (2002) 2480-2486
14. O'Brien-Simpson N., Pathirana R., Paolini R., Chen Y.-Y., Veith P.D., Tam V., et al. An immune response directed to proteinase and adhesin functional epitopes protects against Porphyromonas gingivalis-induced periodontal bone loss. J Immunol 175 6 (2004) 3980-3989
15. O'Brien-Simpson N.M., Black C.L., Bhogal P.S., Cleal S.M., Slakeski N., Higgins T.J., et al. Serum IgG and IgG subclass responses to the RgpA-Kgp proteinase-adhesin complex of P. gingivalis in adult periodontitis. Infect Immun 68 5 (2000) 2704-2712
16. Inagaki S., Ishihara K., Yasaki Y., Yamada S., and Okuda K. Antibody responses of periodontitis patients to ginigpains of Porphromonas gingivalis. J Periodontol 74 10 (2003) 1432-1439
17. McKee A.S., McDermid A.S., Baskerville A., Dowsett A.B., Ellwood D.C., and Marsh P.D. Effect of hemin on the physiology and virulence of Bacteroides gingivalis W50. Infect Immun 52 2 (1986) 349-355
18. Slots J. Importance of black-pigmented Bacteroides in human periodontal disease. In: Genco R.J., and Mergenhagen S.E. (Eds). Host parasite interactions in periodontal diseases: proceedings of a symposium. Buffalo, New York, 4-6 May 1981 (1982), American Society for Microbiology, Washington, DC 27-45
19. Filip C., Fletcher G., Wulff J.L., and Earhart C.F. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium-lauryl sarcosinate. J Bacteriol 115 3 (1973) 717-722
20. Pathirana R.D., O'Brien-Simpson N.M., Veith P.D., Riley P.F., and Reynolds E.C. Characterization of proteinase-adhesin complexes of Porphyromonas gingivalis. Microbiology 152 (2006) 2381-2394
21. Dashper S.G., O'Brien-Simpson N.M., Bhogal P.S., Franzmann A.D., and Reynolds E.C. Purification and characterization of a putative fimbrial protein/receptor of Porphyromonas gingivalis. Aust Dent J 43 2 (1998) 99-104
22. Mortz E., Krogh T.N., Vorum H., and Gorg A. Improved silver staining protocols for high sensitivity protein identification using matrix-assisted laser desorption/ionization-time of flight analysis. Proteomics 1 11 (2001) 1359-1363
23. Norusis M.J. SPPS for Windows: base systems user's guide. Release 6.0 (1993), SPSS Inc., Chicago, IL, USA
24. Cohen J. Statistical power analysis for the behavioral sciences (1969), Academic Press, New York
25. Nguyen K.A., DeCarlo A.A., Paramaesvaran M., Collyer C.A., Langley D.B., and Hunter N. Humoral responses to Porphyromonas gingivalis gingipain adhesin domains in subjects with chronic periodontitis. Infect Immun 72 3 (2004) 1374-1382
26. Yonezawa H., Ishihara K., and Okuda K. Arg-gingipain a DNA vaccine induces protective immunity against infection by Porphyromonas gingivalis in a murine model. Infect Immun 69 5 (2001) 2858-2864
27. DeCarlo A.A., Huang Y., Collyer C.A., Langley D.B., and Katz J. Feasibility of an HA2 domain-based periodontitis vaccine. Infect Immun 71 1 (2003) 562-566