Generation of gene deletions and gene replacements in Escherichia coli O157:H7 using a temperature sensitive allelic exchange system

Biological Procedures Online

Volumn 8, Issue 1, 2006, Pages 153-162

  Emmerson, James R ^a   Gally, David L ^a   Roe, Andrew J ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALLELE; ARTICLE; BACTERIAL GENE; DNA FLANKING REGION; DNA VECTOR; ESCHERICHIA COLI O157; GENE DELETION; GENE TECHNOLOGY; GENETIC TRANSCRIPTION; MOLECULAR CLONING; NONHUMAN; OPERON; PROTEIN LOCALIZATION; REPORTER GENE; TEMPERATURE SENSITIVITY;

ESCHERICHIA COLI;

EID: 33749411092     PISSN: 14809222     EISSN: 14809222     Source Type: Journal    
DOI: 10.1251/bpo123     Document Type: Article

References (19)

1. Falkow S. Molecular Koch's postulates applied to bacterial pathogenicity - a personal recollection 15 years later. Nat Rev Microbiol 1994; 2:67-72.
2. Miller JH. Experiments in Molecular Genetics. New York: Cold Spring Harbour Laboratory Press; 1972.
3. Parker B, Marinus MG. A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation. Gene 1988; 73:531-535.
4. Datsenko KA, Wanner BL. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 2000; 97:6640-6645.
5. Perna NT et al. Genome sequence of enterohaemorrhagic Escherichia coli O157:H7. Nature 2001; 409:529-533.
6. Spears KJ, Roe AJ, Gally DL. A comparison of enteropathogenic and enterohaemorrhagic Escherichia coli pathogenesis. FEMS Microbiol Letts 2005; 255:187-202.
7. Campellone KG, Robbins D, Leong JM. EspF(U) is a translocated EHEC effector that interacts with Tir and N-WASP and promotes nck-independent actin assembly. Dev Cell 2004; 7:217-228.
8. Roe AJ et al. Heterogeneous surface expression of EspA translocon filaments by Escherichia coli O157:H7 is controlled at the posttranscriptional level. Infect Immun 2003; 71:5900-5909.
9. Naylor SW et al. Escherichia coli O157:H7 forms attaching and effacing lesions at the terminal rectum of cattle and colonization requires the LEE4 operon. Microbiol 2005; 151:2773-2781.
10. Cohen SN, Chang ACY. Revised Interpretation of the Origin of the pSC101 Plasmid. J Bacteriol 1997; 132:734-737.
11. Hashimoto-Gotoh TSM, Sekiguchi M. Mutations to Temperature Sensitivity in R Plasmid pSC101. J Bacteriol 1977; 131:405-412.
12. Hamilton CM et al. New method for generating deletions and gene replacements in Escherichia coli. J Bacteriol 1989; 171:4617-4622.
13. Blomfield IC et al. Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature-sensitive pSC101 replicon. Mol Microbiol 1991; 5:1447-1457.
14. Merlin C et al. Tools for characterization of Escherichia coli genes of unknown function. J Bacteriol 2002; 184:4573-4581.
15. Low AS et al. Analysis of fimbrial gene clusters and their expression in enterohaemorrhagic Escherichia coli O157:H7. Env Microbiol 2006; 8:1033-1047.
16. Roe AJ et al. Co-ordinate single-cell expression of LEE4- and LEE5-encoded proteins of Escherichia coli O157:H7. Mol Microbiol 2004; 54:337-352.
17. Porter ME et al. The LEE1 promoters from both enteropathogenic and enterohemorrhagic Escherichia coli can be activated by PerC-like proteins from either organism. J Bacteriol 2005; 187:458-472.
18. Tsunekawa H et al. Acquisition of a sucrose utilization system in Escherichia coli K-12 derivatives and its application to industry. Appl Environ Microbiol 1992; 58:2081-2088.
19. Johnson JR et al. High-Frequency Secondary Mutations after Suicide-Driven Allelic Exchange Mutagenesis in Extraintestinal Pathogenic Escherichia coli. J Bacteriol 2003; 185:5301-5305.