Trapping the tetrahedral intermediate in the alkaline phosphatase reaction by substitution of the active site serine with threonine

Protein Science

Volumn 15, Issue 10, 2006, Pages 2395-2401

  Wang, Jie ^a   Kantrowitz, Evan R ^a  

^a BOSTON COLLEGE   (United States)

Author keywords

Covalent intermediate; Mutagenesis; Rate determining step; Side chain conformation; X ray crystallography

Indexed keywords

ALKALINE PHOSPHATASE; PHOSPHORUS; SERINE; THREONINE;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; ENZYME STRUCTURE; ESCHERICHIA COLI; HYDROLYSIS; MUTAGENESIS; PRIORITY JOURNAL; STEADY STATE; STEREOCHEMISTRY; X RAY CRYSTALLOGRAPHY;

ALKALINE PHOSPHATASE; AMINO ACID SUBSTITUTION; BINDING SITES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI PROTEINS; KINETICS; SERINE; THERMODYNAMICS; THREONINE;

ESCHERICHIA COLI;

EID: 33749333378     PISSN: 09618368     EISSN: 1469896X     Source Type: Journal    
DOI: 10.1110/ps.062351506     Document Type: Article

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